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学科主题: 临床医学
题名:
In vitro resistance to interferon-alpha of hepatitis B virus with basic core promoter double mutation
作者: Wang, Yan; Wei, Lai; Jiang, Dong; Cong, Xu; Fei, Ran; Chen, Hongsong; Xiao, Jiang; Wang, Yu
关键词: hepatitis B virus ; basic core promoter ; mutation ; interferon-alpha
刊名: ANTIVIRAL RESEARCH
发表日期: 2007-08-01
DOI: 10.1016/j.antiviral.2007.02.001
卷: 75, 期:2, 页:139-145
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy ; Virology
研究领域[WOS]: Pharmacology & Pharmacy ; Virology
关键词[WOS]: GENE-EXPRESSION ; E-ANTIGEN ; VIRAL REPLICATION ; ADEFOVIR DIPIVOXIL ; LIVER-DAMAGE ; PRE-C ; PRECORE ; TRANSCRIPTION ; CELLS ; RNA
英文摘要:

The hepatitis B virus (HBV) genome basic core promoter (BCP) modulates HBeAg secretion at the transcriptional level. In addition to pre-core mutations, variations in the BCP are related to hepatitis B e antigen (HBeAg)-negative chronic hepatitis B. HBeAg-negative chronic hepatitis B patients show a lower sustained response to interferon (IFN). The aim of this study was to determine if there is a relationship between HBV BCP mutation and sensitivity of HBV to IFN-alpha in vitro. BCP mutations were introduced by site-directed mutagenesis and the entire genomes of wild-type and mutant HBV were transiently transferred into Huh7 cells by calcium phosphate transfection. With or without IFN-alpha, viral products in the culture medium and viral replication intermediates in the cytoplasm were detected 3 days after transfection. The amount of hepatitis B surface antigen (HBsAg) secreted by wild-type HBV and the BCP mutant was similar, while HBeAg secreted by the mutant was decreased by 35.4%. HBV particles and replication intermediates of the BCP mutant were increased. After IFN-alpha was added, HBeAg, HBV DNA and HBV replication intermediates decreased for both the wild-type HBV (by 25.7%, 31.8%, 29.8%, respectively) and the BCP mutant (by 8.4%, 27.4%, 10.1 %, respectively). These data indicate that HBV harboring the BCP double mutation has stronger replication competence and lower sensitivity to IFN-alpha than wild-type. (C) 2007 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000246956500006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65001
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China

Recommended Citation:
Wang, Yan,Wei, Lai,Jiang, Dong,et al. In vitro resistance to interferon-alpha of hepatitis B virus with basic core promoter double mutation[J]. ANTIVIRAL RESEARCH,2007,75(2):139-145.
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