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FAM170B, a Novel Acrosomal Protein Involved in Fertilization in Mice
Li, Yuchi1,2; Lin, Shouren1; Luo, Manling1,2; Guo, Huan1,3; Chen, Jianbo1,4; Ma, Qian1; Gu, Yanli1; Jiang, Zhimao1; Gui, Yaoting1
刊名MOLECULAR REPRODUCTION AND DEVELOPMENT
2015-10-01
DOI10.1002/mrd.22523
82期:10页:787-796
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology ; Developmental Biology ; Reproductive Biology
资助者National Natural Science Foundation of China ; Shenzhen Project of Science and Technology ; National Natural Science Foundation of China ; Shenzhen Project of Science and Technology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology ; Developmental Biology ; Reproductive Biology
关键词[WOS]MAMMALIAN SPERM ; SPERMATOGENIC CELLS ; SENSITIVE FACTOR ; MALE-INFERTILITY ; MALE-FERTILITY ; GLOBOZOOSPERMIA ; IDENTIFICATION ; BIOGENESIS ; EXOCYTOSIS ; EXPRESSION
英文摘要

The acrosome is a specialized organelle that covers the anterior region of the sperm nucleus, and plays an essential role in mammalian fertilization. Although acrosome biogenesis is an important aspect of spermiogenesis, the molecular mechanism that regulates this event remains unknown. In the present study, we identified a novel gene, Fam170b (family with sequence similarity 170, member B), exclusively expressed in mouse testes. Fam170b expression first started at postnatal week 3, and increased in an age-dependent manner until plateauing in adulthood. Immunofluorescence staining revealed its enrichment in round spermatids, and redistribution to a perinuclear spot adjacent to the Golgi and the acrosome of elongating spermatids and spermatozoa; this localization was shared between mouse and human spermatozoa. Anti-FAM170B antibody was remarkably found to inhibit murine in vitro fertilization, specifically blocking the acrosome reaction. We further determined that FAM170B interacts with GOPC (Golgi-associated PDZ and coiled-coil motif containing protein) during acrosome formation, as verified by immunofluorescence and co-immunoprecipitation assays. Thus, we document the expression and function for the endogenous acrosomal protein FAM170B during spermiogenesis and fertilization. (C) 2015 Wiley Periodicals, Inc.

语种英语
所属项目编号31271244 ; 31471344 ; XB201104220045A ; JCYJ20140415162543017 ; 201302042
资助者National Natural Science Foundation of China ; Shenzhen Project of Science and Technology ; National Natural Science Foundation of China ; Shenzhen Project of Science and Technology
WOS记录号WOS:000362982100008
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65005
专题北京大学深圳医院
作者单位1.Peking Univ, Shenzhen PKU HKUST Med Ctr, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol,Shenzhen Hosp, Shenzhen 518036, Peoples R China
2.Shantou Univ, Coll Med, Shantou, Peoples R China
3.Guangzhou Med Univ, Guangzhou, Guangdong, Peoples R China
4.Anhui Med Univ, Hefei, Peoples R China
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Li, Yuchi,Lin, Shouren,Luo, Manling,et al. FAM170B, a Novel Acrosomal Protein Involved in Fertilization in Mice[J]. MOLECULAR REPRODUCTION AND DEVELOPMENT,2015,82(10):787-796.
APA Li, Yuchi.,Lin, Shouren.,Luo, Manling.,Guo, Huan.,Chen, Jianbo.,...&Gui, Yaoting.(2015).FAM170B, a Novel Acrosomal Protein Involved in Fertilization in Mice.MOLECULAR REPRODUCTION AND DEVELOPMENT,82(10),787-796.
MLA Li, Yuchi,et al."FAM170B, a Novel Acrosomal Protein Involved in Fertilization in Mice".MOLECULAR REPRODUCTION AND DEVELOPMENT 82.10(2015):787-796.
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