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学科主题: 公共卫生
题名:
Grape seed procyanidin B2 ameliorates mitochondrial dysfunction and inhibits apoptosis via the AMP-activated protein kinase-silent mating type information regulation 2 homologue 1-PPAR gamma co-activator-1 alpha axis in rat mesangial cells under high-dose glucosamine
作者: Bao, Lei; Cai, Xiaxia; Zhang, Zhaofeng; Li, Yong
关键词: Apoptosis ; Diabetic nephropathy ; Grape seed procyanidin B2 ; Mesangial cells ; Mitochondrial dysfunction
刊名: BRITISH JOURNAL OF NUTRITION
发表日期: 2015-01-14
DOI: 10.1017/S000711451400347X
卷: 113, 期:1, 页:35-44
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Nutrition & Dietetics
研究领域[WOS]: Nutrition & Dietetics
关键词[WOS]: INDUCED DIABETIC-RATS ; OXIDATIVE STRESS ; PROANTHOCYANIDIN EXTRACTS ; DNA-DAMAGE ; DB/DB MICE ; COACTIVATOR ; NUCLEAR ; PATHWAY ; INJURY ; DEATH
英文摘要:

Grape seed procyanidin B2 (GSPB2), an antioxidative and anti-inflammatory polyphenol in grape seed, has been found to have protective effects on diabetic nephropathy. Based on its favourable biological activities, in the present study, we aimed to investigate whether GSPB2 could inhibit apoptosis in rat mesangial cells treated with glucosamine (GlcN) under high-dose conditions. The results showed that the administration of GSPB2 (10 mu g/ml) significantly increased the viability of mesangial cells treated with GlcN at a dose of 15 mm. We found that GSPB2 inhibited apoptosis in mesangial cells using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphates (dUTP) nick-end labelling staining and flow cytometry technique (P<0.05 for both). GSPB2 treatment also suppressed oxidative stress by elevating the activity of glutathione peroxidase (P<0.05) and superoxide dismutase (P<0.01), as well as prevented cellular damage. GSPB2 enhanced the mRNA expression of nuclear respiratory factor 1, mitochondrial transcription factor A and mitochondrial DNA copy number in mesangial cells as determined by real-time PCR (P<0.05 for each). Finally, GSPB2 treatment activated the protein expression of PPAR? co-activator-1 alpha (PGC-1 alpha), silent mating type information regulation 2 homologue 1 (SIRT1) and AMP-activated protein kinase (AMPK) in mesangial cells. These findings suggest that GSPB2 markedly ameliorates mitochondrial dysfunction and inhibits apoptosis in rat mesangial cells treated with high-dose GlcN. This protective effect could be, at least in part, due to the activation of the AMPKSIRT1PGC-1 alpha axis.

语种: 英语
所属项目编号: 81102116 ; 81072293 ; 20110001120026
项目资助者: National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education
WOS记录号: WOS:000347105000004
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65007
Appears in Collections:北京大学公共卫生学院_期刊论文

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作者单位: Peking Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Beijing 100191, Peoples R China

Recommended Citation:
Bao, Lei,Cai, Xiaxia,Zhang, Zhaofeng,et al. Grape seed procyanidin B2 ameliorates mitochondrial dysfunction and inhibits apoptosis via the AMP-activated protein kinase-silent mating type information regulation 2 homologue 1-PPAR gamma co-activator-1 alpha axis in rat mesangial cells under high-dose glucosamine[J]. BRITISH JOURNAL OF NUTRITION,2015,113(1):35-44.
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