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Grape seed procyanidin B2 ameliorates mitochondrial dysfunction and inhibits apoptosis via the AMP-activated protein kinase-silent mating type information regulation 2 homologue 1-PPAR gamma co-activator-1 alpha axis in rat mesangial cells under high-dose glucosamine
Bao, Lei; Cai, Xiaxia; Zhang, Zhaofeng; Li, Yong
关键词Apoptosis Diabetic nephropathy Grape seed procyanidin B2 Mesangial cells Mitochondrial dysfunction
刊名BRITISH JOURNAL OF NUTRITION
2015-01-14
DOI10.1017/S000711451400347X
113期:1页:35-44
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Nutrition & Dietetics
资助者National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education
研究领域[WOS]Nutrition & Dietetics
关键词[WOS]INDUCED DIABETIC-RATS ; OXIDATIVE STRESS ; PROANTHOCYANIDIN EXTRACTS ; DNA-DAMAGE ; DB/DB MICE ; COACTIVATOR ; NUCLEAR ; PATHWAY ; INJURY ; DEATH
英文摘要

Grape seed procyanidin B2 (GSPB2), an antioxidative and anti-inflammatory polyphenol in grape seed, has been found to have protective effects on diabetic nephropathy. Based on its favourable biological activities, in the present study, we aimed to investigate whether GSPB2 could inhibit apoptosis in rat mesangial cells treated with glucosamine (GlcN) under high-dose conditions. The results showed that the administration of GSPB2 (10 mu g/ml) significantly increased the viability of mesangial cells treated with GlcN at a dose of 15 mm. We found that GSPB2 inhibited apoptosis in mesangial cells using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphates (dUTP) nick-end labelling staining and flow cytometry technique (P<0.05 for both). GSPB2 treatment also suppressed oxidative stress by elevating the activity of glutathione peroxidase (P<0.05) and superoxide dismutase (P<0.01), as well as prevented cellular damage. GSPB2 enhanced the mRNA expression of nuclear respiratory factor 1, mitochondrial transcription factor A and mitochondrial DNA copy number in mesangial cells as determined by real-time PCR (P<0.05 for each). Finally, GSPB2 treatment activated the protein expression of PPAR? co-activator-1 alpha (PGC-1 alpha), silent mating type information regulation 2 homologue 1 (SIRT1) and AMP-activated protein kinase (AMPK) in mesangial cells. These findings suggest that GSPB2 markedly ameliorates mitochondrial dysfunction and inhibits apoptosis in rat mesangial cells treated with high-dose GlcN. This protective effect could be, at least in part, due to the activation of the AMPKSIRT1PGC-1 alpha axis.

语种英语
所属项目编号81102116 ; 81072293 ; 20110001120026
资助者National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education ; National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education
WOS记录号WOS:000347105000004
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65007
专题北京大学公共卫生学院
作者单位Peking Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Bao, Lei,Cai, Xiaxia,Zhang, Zhaofeng,et al. Grape seed procyanidin B2 ameliorates mitochondrial dysfunction and inhibits apoptosis via the AMP-activated protein kinase-silent mating type information regulation 2 homologue 1-PPAR gamma co-activator-1 alpha axis in rat mesangial cells under high-dose glucosamine[J]. BRITISH JOURNAL OF NUTRITION,2015,113(1):35-44.
APA Bao, Lei,Cai, Xiaxia,Zhang, Zhaofeng,&Li, Yong.(2015).Grape seed procyanidin B2 ameliorates mitochondrial dysfunction and inhibits apoptosis via the AMP-activated protein kinase-silent mating type information regulation 2 homologue 1-PPAR gamma co-activator-1 alpha axis in rat mesangial cells under high-dose glucosamine.BRITISH JOURNAL OF NUTRITION,113(1),35-44.
MLA Bao, Lei,et al."Grape seed procyanidin B2 ameliorates mitochondrial dysfunction and inhibits apoptosis via the AMP-activated protein kinase-silent mating type information regulation 2 homologue 1-PPAR gamma co-activator-1 alpha axis in rat mesangial cells under high-dose glucosamine".BRITISH JOURNAL OF NUTRITION 113.1(2015):35-44.
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