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学科主题: 临床医学
题名:
Simplified method of heterotopic rat heart transplantation using the cuff technique: Application to sublethal dose protocol of methotrexate on allograft survival
作者: Xiu, DR; Uchida, H; To, H; Sugimoto, K; Kasahara, K; Nagai, H; Fujimura, A; Kobayashi, E
刊名: MICROSURGERY
发表日期: 2001
卷: 21, 期:1, 页:16-21
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Surgery
研究领域[WOS]: Surgery
关键词[WOS]: PRIMARY BILIARY-CIRRHOSIS ; LIVER-TRANSPLANTATION ; RHEUMATOID-ARTHRITIS ; SIMPLIFIED MODEL ; THERAPY ; DISEASE
英文摘要:

Rodent heterotopic heart transplantation (HHT) models have been developed for the study of transplantation immunology. Most of these transplantations are performed by hand-suture techniques, requiring several months of training. We describe a modified technique of rat HHT in the neck, using a cuff method that can be mastered by beginners within a few weeks. Our main modification of the rat HHT in the neck is that the right superior vena cava of the graft is chosen as an outflow duct, while the pulmonary artery has been taken as an effluent drainage in the ordinary HHT models. The aorta of the donor is anastomosed with the carotid artery of the recipient. Donation can be completed within 5 min and vascular connections in the recipient done within 3 min, resulting in a minimum of ischemia time. Using this minimum surgical intervention model, we tested the immunosuppressive effect of a sublethal dose of methotrexate (MTX), which has been widely used in cancer therapy. Our results showed that high doses of MTX severely suppressed the recipient bone marrow, but prolonged heart allografts for more than 365 days after HHT. In conclusion, the new model simplified the rat HHT procedure and made it possible for the beginner of rodent transplantation to master this skill within a few weeks. Using this minimized intervention technique, we found that the high doses of MTX can significantly prolong the survival of fully mismatched DA heart graft in PVG/c recipient. (C) 2001 Wiley-Liss, Inc.

语种: 英语
WOS记录号: WOS:000166775400004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65024
Appears in Collections:北京大学第三临床医学院_普通外科_期刊论文

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作者单位: 1.Jichi Med Sch, Ctr Mol Med, Div Organ Replacement Res, Minami Kawachi, Tochigi 3290498, Japan
2.Jichi Med Sch, Ctr Mol Med, Dept Surg, Minami Kawachi, Tochigi 3290498, Japan
3.Beijing Med Univ, Teaching Hosp 3, Dept Gen Surg, Beijing 100083, Peoples R China

Recommended Citation:
Xiu, DR,Uchida, H,To, H,et al. Simplified method of heterotopic rat heart transplantation using the cuff technique: Application to sublethal dose protocol of methotrexate on allograft survival[J]. MICROSURGERY,2001,21(1):16-21.
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