北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 眼科  > 期刊论文
学科主题: 临床医学
题名:
Nerve growth factor and its receptor TrkA serve as potential markers for human corneal epithelial progenitor cells
作者: Qi, Hong1,4; Li, De-Quan1; Shine, H. David2,3; Chen, Zhuo1; Yoon, Kyung-Chul1,5; Jones, Dan B.1; Pflugfelder, Stephen C.1
关键词: cornea ; epithelium ; progenitor cell ; nerve growth factor ; nerve growth factor receptor ; neurotrophin ; TrkA ; p75
刊名: EXPERIMENTAL EYE RESEARCH
发表日期: 2008
DOI: 10.1016/j.exer.2007.09.003
卷: 86, 期:1, 页:34-40
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: STEM-CELLS ; OCULAR SURFACE ; TOPICAL TREATMENT ; LIMBAL ; POPULATION ; EXPRESSION ; P75(NTR) ; DIFFERENTIATION ; NEUROTROPHINS ; CULTURE
英文摘要:

Nerve growth factor (NGF), a member of the neurotrophin family, has been identified as an essential growth factor supporting stem cell self-renewal outside the nervous system and was previously shown to stimulate corneal epithelial proliferation both in vivo and in vitro. In this study, we evaluated the expression of NGF and its corresponding receptors in the human corneal and limbal tissues, as well as in primary limbal epithelial cultures by immunofluorescent staining and relatively quantitative real-time polymerase chain reaction. We found that NGF was uniquely expressed in the human limbal basal epithelium, together with its two corresponding receptors: the high-affinity receptor TrkA and the low-affinity receptor p75NTR. TrkA was shown to preferentially localize to limbal basal epithelial cells. NGF and TrkA were also found co-localized with stem cell-associated molecular markers (drug-resistance transporter ABCG2 and p63), but not with the differentiation marker cytokeratin 3 in the human limbal basal epithelial layer. In cultured limbal epithelial cells, NGF and TrkA were found to be preferentially expressed by a small population of limbal epithelial cells. The NGF and TrkA immuno-positive subpopulations were enriched for certain properties (including ABCG2 and p63 expression) of putative limbal epithelial stem cells (P < 0.01, compared with the entire cell population). Levels of NGF and TrkA transcripts were found to be much more abundant in limbal than in corneal tissues, and in young cultured cells in the proliferative stage than in airlifted stratified cultures containing differentiated cells. The co-expression of NGF with its two corresponding receptors in limbal basal epithelial cells. but not in the cornea, suggests that NGF may function as a critical autocrine or paracrine factor supporting stem cell self-renewal in the limbal stem cell niche. The spatial expression of NGF and TrkA by small clusters of basal cells interspersed between negative cell patches suggests that they are potential markets for human corneal epithelial progenitor cells. (C) 2007 Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000252209100005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65028
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Baylor Coll Med, Dept Ophthalmol, Cullen Eye Inst, Ocular Surface Ctr, Houston, TX 77030 USA
2.Baylor Coll Med, Ctr Cell & Gene Therapy, Houston, TX 77030 USA
3.Baylor Coll Med, Dept Neurosurg, Houston, TX 77030 USA
4.Peking Univ, Hosp 3, Ctr Eye, Beijing 100871, Peoples R China
5.Chonnam Natl Univ, Med Sch & Hosp, Dept Ophthalmol, Kwangju, South Korea

Recommended Citation:
Qi, Hong,Li, De-Quan,Shine, H. David,et al. Nerve growth factor and its receptor TrkA serve as potential markers for human corneal epithelial progenitor cells[J]. EXPERIMENTAL EYE RESEARCH,2008,86(1):34-40.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Qi, Hong]'s Articles
[Li, De-Quan]'s Articles
[Shine, H. David]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Qi, Hong]‘s Articles
[Li, De-Quan]‘s Articles
[Shine, H. David]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace