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学科主题临床医学
Nerve growth factor and its receptor TrkA serve as potential markers for human corneal epithelial progenitor cells
Qi, Hong1,4; Li, De-Quan1; Shine, H. David2,3; Chen, Zhuo1; Yoon, Kyung-Chul1,5; Jones, Dan B.1; Pflugfelder, Stephen C.1
关键词cornea epithelium progenitor cell nerve growth factor nerve growth factor receptor neurotrophin TrkA p75
刊名EXPERIMENTAL EYE RESEARCH
2008
DOI10.1016/j.exer.2007.09.003
86期:1页:34-40
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]STEM-CELLS ; OCULAR SURFACE ; TOPICAL TREATMENT ; LIMBAL ; POPULATION ; EXPRESSION ; P75(NTR) ; DIFFERENTIATION ; NEUROTROPHINS ; CULTURE
英文摘要

Nerve growth factor (NGF), a member of the neurotrophin family, has been identified as an essential growth factor supporting stem cell self-renewal outside the nervous system and was previously shown to stimulate corneal epithelial proliferation both in vivo and in vitro. In this study, we evaluated the expression of NGF and its corresponding receptors in the human corneal and limbal tissues, as well as in primary limbal epithelial cultures by immunofluorescent staining and relatively quantitative real-time polymerase chain reaction. We found that NGF was uniquely expressed in the human limbal basal epithelium, together with its two corresponding receptors: the high-affinity receptor TrkA and the low-affinity receptor p75NTR. TrkA was shown to preferentially localize to limbal basal epithelial cells. NGF and TrkA were also found co-localized with stem cell-associated molecular markers (drug-resistance transporter ABCG2 and p63), but not with the differentiation marker cytokeratin 3 in the human limbal basal epithelial layer. In cultured limbal epithelial cells, NGF and TrkA were found to be preferentially expressed by a small population of limbal epithelial cells. The NGF and TrkA immuno-positive subpopulations were enriched for certain properties (including ABCG2 and p63 expression) of putative limbal epithelial stem cells (P < 0.01, compared with the entire cell population). Levels of NGF and TrkA transcripts were found to be much more abundant in limbal than in corneal tissues, and in young cultured cells in the proliferative stage than in airlifted stratified cultures containing differentiated cells. The co-expression of NGF with its two corresponding receptors in limbal basal epithelial cells. but not in the cornea, suggests that NGF may function as a critical autocrine or paracrine factor supporting stem cell self-renewal in the limbal stem cell niche. The spatial expression of NGF and TrkA by small clusters of basal cells interspersed between negative cell patches suggests that they are potential markets for human corneal epithelial progenitor cells. (C) 2007 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000252209100005
引用统计
被引频次:53[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65028
专题北京大学第三临床医学院_眼科
作者单位1.Baylor Coll Med, Dept Ophthalmol, Cullen Eye Inst, Ocular Surface Ctr, Houston, TX 77030 USA
2.Baylor Coll Med, Ctr Cell & Gene Therapy, Houston, TX 77030 USA
3.Baylor Coll Med, Dept Neurosurg, Houston, TX 77030 USA
4.Peking Univ, Hosp 3, Ctr Eye, Beijing 100871, Peoples R China
5.Chonnam Natl Univ, Med Sch & Hosp, Dept Ophthalmol, Kwangju, South Korea
推荐引用方式
GB/T 7714
Qi, Hong,Li, De-Quan,Shine, H. David,et al. Nerve growth factor and its receptor TrkA serve as potential markers for human corneal epithelial progenitor cells[J]. EXPERIMENTAL EYE RESEARCH,2008,86(1):34-40.
APA Qi, Hong.,Li, De-Quan.,Shine, H. David.,Chen, Zhuo.,Yoon, Kyung-Chul.,...&Pflugfelder, Stephen C..(2008).Nerve growth factor and its receptor TrkA serve as potential markers for human corneal epithelial progenitor cells.EXPERIMENTAL EYE RESEARCH,86(1),34-40.
MLA Qi, Hong,et al."Nerve growth factor and its receptor TrkA serve as potential markers for human corneal epithelial progenitor cells".EXPERIMENTAL EYE RESEARCH 86.1(2008):34-40.
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