学科主题基础医学
Maintenance of human embryonic stem cells on gelatin
Li Yang1,2; Lin ChangSheng1,2; Wang Li1,2; Liu Ying1,2,3; Mu XiaoNing4; Ma Yue4; Li LingSong1,2
关键词human embryonic stem cells gelatin Matrigel feeder-free pluripotency
刊名CHINESE SCIENCE BULLETIN
2009-11-01
DOI10.1007/s11434-009-0630-4
54期:22页:4214-4220
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]HUMAN BLASTOCYSTS ; DIFFERENTIATION ; LINES ; PROLIFERATION ; PLURIPOTENCY ; CULTURE ; GROWTH
英文摘要

Matrigel is routinely used as a coating material in the feeder-free culture system of human embryonic stem cells (hESCs). However, matrigel is costive and inconvenient to use. In this study, the possibility of using gelatin as an alternative coating material was investigated. The results showed that, after trypsinization, hESCs were maintained undifferentiated on gelatin. These hESCs expressed pluripotent markers, formed teratoma and maintained a normal karyotype. As measured at passage 10, the hESCs expressed a high level of 004 on both gelatin and Matrigel. hESCs growing on gelatin formed AP-positive colonies in similar size and number to those growing on Matrigel (P > 0.05). Moreover, hESCs growing on gelatin contained a comparable percentage of SSEA-4-positive cells to those growing on Matrigel (95.1% vs.94.3%, P>0.05). H-1 hESCs were maintained undifferentiated on gelatin for 20 passages and remained the stable normal karyotype. This gelatin-based culture protocol may allow us to propagate hESCs in large scale, with less cost.

语种英语
WOS记录号WOS:000272034800016
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65041
专题北京大学基础医学院_北京大学干细胞研究中心
作者单位1.Peking Univ, Stem Cell Res Ctr, Beijing 100191, Peoples R China
2.China Natl Ctr Int Res, Beijing 100191, Peoples R China
3.Beijing Obstet & Genecol Hosp, Dept Reprod, Beijing 100026, Peoples R China
4.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
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GB/T 7714
Li Yang,Lin ChangSheng,Wang Li,et al. Maintenance of human embryonic stem cells on gelatin[J]. CHINESE SCIENCE BULLETIN,2009,54(22):4214-4220.
APA Li Yang.,Lin ChangSheng.,Wang Li.,Liu Ying.,Mu XiaoNing.,...&Li LingSong.(2009).Maintenance of human embryonic stem cells on gelatin.CHINESE SCIENCE BULLETIN,54(22),4214-4220.
MLA Li Yang,et al."Maintenance of human embryonic stem cells on gelatin".CHINESE SCIENCE BULLETIN 54.22(2009):4214-4220.
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