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学科主题临床医学
Clinical application of radioimmunoguided surgery in colorectal cancer using I-125-labeled carcinoembryonic antigen-specific monoclonal antibody submucosally
Gu, J; Zhao, J; Li, ZF; Yang, Z; Zhang, JC; Gao, ZA; Wang, Y; Xu, GW
刊名DISEASES OF THE COLON & RECTUM
2003-12-01
DOI10.1097/01.DCR.0000098924.21144.33
46期:12页:1659-1666
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology ; Surgery
研究领域[WOS]Gastroenterology & Hepatology ; Surgery
关键词[WOS]LYMPH-NODES ; CARCINOMA
英文摘要

PURPOSE: The aim of this study was to evaluate the applicability of I-125-labeled carcinoembryonic antigen-specific monoclonal antibody CL58 in clinical radioimmunoguided surgery for colorectal cancer. METHODS: First, we tested the purity and affinity constant of CL58 and measured the binding affinity of CL58 to colorectal cancer cells and normal cells. Second, we injected I-125-labeled CL58 into nude mice with colon cancers. Then, samples from the tumor, blood, and normal tissues of injected mice were weighed and counted in a gamma-ray counter for assessment of biodistribution. Finally, we administered I-125-labeled CL58 submucosally in 29 patients with colorectal cancer via endoscope. Radioimmunoguided surgery was performed 3 to 14 days later with a portable gamma-detecting probe to obtain the counts in the target sites. Tumor-to-normal tissue ratio of 3 was taken as the lowest positive threshold value for primary lesions, wall infiltration, and lymph node metastasis. In addition, all the samples were examined by routine histopathology. Lymph nodes negative by routine histopathology were subjected to immunohistochemical staining with anticytokeratin to detect the lymphatic micrometastasis. RESULTS: The affinity constant of CL58 was 7.5 X 10(9) M-. Moreover, CL58 reacted strongly to the colorectal cancer cell lines, but not to the normal control cells. Furthermore, the tumor tissues showed significant intake of I-125-labeled CL58, as compared with that of normal tissues. The sensitivity of radioimmunoguided surgery in detecting primary lesions was 93.1 percent, and the specificity of radioimmunoguided surgery to correctly identify negative incisional margins for tumor infiltration was 95.5 percent. For the detection of lymphatic metastasis, the sensitivity of radioimmunoguided surgery was 92.0 percent and the specificity was 87.8 percent. The sensitivity of radioimmunoguided surgery in detecting lymph node metastasis was significantly higher when compared with traditional clinical methods (P = 0.0087). The specificity of radioimmunoguided surgery to identify negative incisional margins was also significantly higher when compared with traditional clinical methods (P = 0.0117). The sensitivity and specificity of radioimmunoguided surgery in detecting lymph nodes metastasis showed statistical significance, as compared with traditional clinical methods. Immunohistochemistry verified the existence of lymphatic micrometastasis in radioimmunoguided surgery-positive but histology-negative lymph nodes. CONCLUSIONS: This study indicates that radioimmunoguided surgery for colorectal cancer using I-125-labeled anticarcinoembryonic antigen monoclonal antibody submucosally enables surgeons to define lymphatic metastasis, thus successfully guiding surgeons in performing personalized radical operation.

语种英语
WOS记录号WOS:000187293100012
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65042
专题北京大学临床肿瘤学院_肿瘤外科
作者单位Peking Univ, Sch Oncol,Beijing Canc Hosp, Inst Canc Res, Dept Surg, Beijing 100036, Peoples R China
推荐引用方式
GB/T 7714
Gu, J,Zhao, J,Li, ZF,et al. Clinical application of radioimmunoguided surgery in colorectal cancer using I-125-labeled carcinoembryonic antigen-specific monoclonal antibody submucosally[J]. DISEASES OF THE COLON & RECTUM,2003,46(12):1659-1666.
APA Gu, J.,Zhao, J.,Li, ZF.,Yang, Z.,Zhang, JC.,...&Xu, GW.(2003).Clinical application of radioimmunoguided surgery in colorectal cancer using I-125-labeled carcinoembryonic antigen-specific monoclonal antibody submucosally.DISEASES OF THE COLON & RECTUM,46(12),1659-1666.
MLA Gu, J,et al."Clinical application of radioimmunoguided surgery in colorectal cancer using I-125-labeled carcinoembryonic antigen-specific monoclonal antibody submucosally".DISEASES OF THE COLON & RECTUM 46.12(2003):1659-1666.
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