IR@PKUHSC  > 北京大学第三临床医学院  > 麻醉科
学科主题临床医学
Cytosolic carboxypeptidase CCP6 is required for megakaryopoiesis by modulating Mad2 polyglutamylation
Ye, Buqing1; Li, Chong1; Yang, Zhao1,6; Wang, Yanying1; Hao, Junfeng2; Wang, Li1; Li, Yi7; Du, Ying1; Hao, Lu1; Liu, Benyu1; Wang, Shuo1; Xia, Pengyan1; Huang, Guanling1; Sun, Lei3; Tian, Yong4,5; Fan, Zusen1
刊名JOURNAL OF EXPERIMENTAL MEDICINE
2014-11-17
DOI10.1084/jem.20141123
211期:12页:2439-2454
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Medicine, Research & Experimental
研究领域[WOS]Immunology ; Research & Experimental Medicine
关键词[WOS]SPINDLE ASSEMBLY CHECKPOINT ; HEMATOPOIETIC STEM-CELLS ; AURORA-B KINASE ; MEGAKARYOCYTE DEVELOPMENT ; ALPHA-TUBULIN ; PLATELET FORMATION ; MAMMALIAN-CELLS ; BETA-TUBULIN ; MICE ; POLYPLOIDIZATION
英文摘要

Bone marrow progenitor cells develop into mature megakaryocytes (MKs) to produce platelets for hemostasis and other physiological functions. However, the molecular mechanisms underlying megakaryopoiesis are not completely defined. We show that cytosolic carboxy-peptidase (CCP) 6 deficiency in mice causes enlarged spleens and increased platelet counts with underdeveloped MKs and dysfunctional platelets. The prominent phenotypes of CCP6 deficiency are different from those of CCP1-deficient mice. We found that CCP6 and tubulin tyrosine ligase-like family (TTLL) members TTLL4 and TTLL6 are highly expressed in MKs. We identify Mad2 (mitotic arrest deficient 2) as a novel substrate for CCP6 and not CCP1. Mad2 can be polyglutamylated by TTLL4 and TTLL6 to modulate the maturation of MKs. CCP6 deficiency causes hyperglutamylation of Mad2 to promote activation of Aurora B, leading to suppression of MK maturation. We reveal that Mad2 polyglutamylation plays a critical role in the regulation of megakaryopoiesis.

语种英语
WOS记录号WOS:000345268800015
项目编号XDA01010407 ; XDA01020203 ; 81330047 ; 30830030 ; 31372404 ; 2010CB911902 ; 2014CB964601 ; 20110490617 ; 2012T50145
资助机构Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Key Basic Research Program of China ; Chinese Academy of Sciences ; China Postdoctoral Science Foundation
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65044
专题北京大学第三临床医学院_麻醉科
作者单位1.Chinese Acad Sci, Inst Biophys, Key Lab Infect & Immun, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Inst Biophys, Lab Anim Res Ctr, Beijing 100101, Peoples R China
3.Chinese Acad Sci, Inst Biophys, Ctr Biol Imaging, Beijing 100101, Peoples R China
4.Chinese Acad Sci, Inst Biophys, Key Lab RNA Biol, Beijing 100101, Peoples R China
5.Chinese Acad Sci, Inst Biophys, Beijing Noncoding RNA Lab, Beijing 100101, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
7.Peking Univ, Hosp 3, Dept Anesthesiol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Ye, Buqing,Li, Chong,Yang, Zhao,et al. Cytosolic carboxypeptidase CCP6 is required for megakaryopoiesis by modulating Mad2 polyglutamylation[J]. JOURNAL OF EXPERIMENTAL MEDICINE,2014,211(12):2439-2454.
APA Ye, Buqing.,Li, Chong.,Yang, Zhao.,Wang, Yanying.,Hao, Junfeng.,...&Fan, Zusen.(2014).Cytosolic carboxypeptidase CCP6 is required for megakaryopoiesis by modulating Mad2 polyglutamylation.JOURNAL OF EXPERIMENTAL MEDICINE,211(12),2439-2454.
MLA Ye, Buqing,et al."Cytosolic carboxypeptidase CCP6 is required for megakaryopoiesis by modulating Mad2 polyglutamylation".JOURNAL OF EXPERIMENTAL MEDICINE 211.12(2014):2439-2454.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Ye, Buqing]的文章
[Li, Chong]的文章
[Yang, Zhao]的文章
百度学术
百度学术中相似的文章
[Ye, Buqing]的文章
[Li, Chong]的文章
[Yang, Zhao]的文章
必应学术
必应学术中相似的文章
[Ye, Buqing]的文章
[Li, Chong]的文章
[Yang, Zhao]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。