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学科主题基础医学
Two-photon fluorescence real-time imaging on the development of early mouse embryo by stages
Liu, X.1; Wang, P.1; Fu, J.1; Lv, D.2; Chen, D.1; Li, Y.2; Ma, W.1
关键词Two-photon laser scanning fluorescence microscopy (TPLSM) mouse embryo real-time cleavage compaction
刊名JOURNAL OF MICROSCOPY
2011-02-01
DOI10.1111/j.1365-2818.2010.03426.x
241期:2页:212-218
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microscopy
研究领域[WOS]Microscopy
关键词[WOS]LASER-SCANNING MICROSCOPY ; EMERGING ASYMMETRY ; CLEAVAGE PERIOD ; CELL-SHAPE ; EXCITATION ; VISUALIZATION ; PHOTODAMAGE ; COMPACTION ; TISSUE ; MODEL
英文摘要

P>Mouse zygotes are widely used in developmental biology and transgenic animal research. Two-photon laser scanning microscopy is particularly useful in four-dimensional observation of big and thick biological samples, such as mouse embryo. The early mouse embryo development from zygote to 8-cell stage compaction was observed in real-time by stages using two-photon laser scanning microscopy in this paper. During our experiment, several scanning parameters were optimized in different development stages. The initial cleavages of mouse embryo, from the zygote to 2-cell, 2-cell to 4-cell, 4-cell to 8-cell, and the compaction at 8-cell stage were observed. During the first stage, localized intracellular calcium elevation along with the cleavage furrow and the asymmetric zygote cytokinesis was detected. The relation between the asymmetry and the location of the second polar body was investigated. The rotational cleavage of mouse embryo was also observed in the experiment. These results would be helpful to further research on mammalian embryonic development.

语种英语
WOS记录号WOS:000286110500014
项目编号90919012 ; 10874099 ; 2007CB5119004 ; 20090002110065
资助机构National Nature Science Foundation of China ; Chinese National Basic Research Program ; Chinese Ministry of Education
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65053
专题北京大学基础医学院
作者单位1.Tsinghua Univ, Dept Phys, Educ Minist, Key Lab Atom & Mol Nanosci, Beijing 100084, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Anat & Histol & Embryol, Beijing 100083, Peoples R China
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GB/T 7714
Liu, X.,Wang, P.,Fu, J.,et al. Two-photon fluorescence real-time imaging on the development of early mouse embryo by stages[J]. JOURNAL OF MICROSCOPY,2011,241(2):212-218.
APA Liu, X..,Wang, P..,Fu, J..,Lv, D..,Chen, D..,...&Ma, W..(2011).Two-photon fluorescence real-time imaging on the development of early mouse embryo by stages.JOURNAL OF MICROSCOPY,241(2),212-218.
MLA Liu, X.,et al."Two-photon fluorescence real-time imaging on the development of early mouse embryo by stages".JOURNAL OF MICROSCOPY 241.2(2011):212-218.
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