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学科主题: 基础医学
题名:
Two-photon fluorescence real-time imaging on the development of early mouse embryo by stages
作者: Liu, X.1; Wang, P.1; Fu, J.1; Lv, D.2; Chen, D.1; Li, Y.2; Ma, W.1
关键词: Two-photon laser scanning fluorescence microscopy (TPLSM) ; mouse embryo ; real-time ; cleavage ; compaction
刊名: JOURNAL OF MICROSCOPY
发表日期: 2011-02-01
DOI: 10.1111/j.1365-2818.2010.03426.x
卷: 241, 期:2, 页:212-218
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microscopy
研究领域[WOS]: Microscopy
关键词[WOS]: LASER-SCANNING MICROSCOPY ; EMERGING ASYMMETRY ; CLEAVAGE PERIOD ; CELL-SHAPE ; EXCITATION ; VISUALIZATION ; PHOTODAMAGE ; COMPACTION ; TISSUE ; MODEL
英文摘要:

P>Mouse zygotes are widely used in developmental biology and transgenic animal research. Two-photon laser scanning microscopy is particularly useful in four-dimensional observation of big and thick biological samples, such as mouse embryo. The early mouse embryo development from zygote to 8-cell stage compaction was observed in real-time by stages using two-photon laser scanning microscopy in this paper. During our experiment, several scanning parameters were optimized in different development stages. The initial cleavages of mouse embryo, from the zygote to 2-cell, 2-cell to 4-cell, 4-cell to 8-cell, and the compaction at 8-cell stage were observed. During the first stage, localized intracellular calcium elevation along with the cleavage furrow and the asymmetric zygote cytokinesis was detected. The relation between the asymmetry and the location of the second polar body was investigated. The rotational cleavage of mouse embryo was also observed in the experiment. These results would be helpful to further research on mammalian embryonic development.

语种: 英语
所属项目编号: 90919012 ; 10874099 ; 2007CB5119004 ; 20090002110065
项目资助者: National Nature Science Foundation of China ; Chinese National Basic Research Program ; Chinese Ministry of Education
WOS记录号: WOS:000286110500014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65053
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Tsinghua Univ, Dept Phys, Educ Minist, Key Lab Atom & Mol Nanosci, Beijing 100084, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Anat & Histol & Embryol, Beijing 100083, Peoples R China

Recommended Citation:
Liu, X.,Wang, P.,Fu, J.,et al. Two-photon fluorescence real-time imaging on the development of early mouse embryo by stages[J]. JOURNAL OF MICROSCOPY,2011,241(2):212-218.
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