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学科主题: 临床医学
题名:
In vitro antitumor immune response induced by fusion of dendritic cells and colon cancer cells
作者: Xu, Feng1,2; Ye, Ying-Jiang1,2; Wang, Shan1,2
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2004-04-15
卷: 10, 期:8, 页:1162-1166
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
英文摘要:

AIM: The prevention of recurrence of colon cancer (CC) after operation is very important for improvement of the prognosis of CC patients, especially those with micro-metastasis. The generation of fused cells between dendritic cells (DCs) and tumor cells maybe an effective approach for tumor antigen presentation in immunotherapy. In this study, we fused human colon caner SW480 cells and human peripheral blood - derived DCs to induce an antitumor activity against human CC.

METHODS: CC SW480 cells and human peripheral blood derived DCs were fused with 500 mL/L polyethylene glycol (PEG).

RESULTS: The specific T cell responses activated by fusion cells (FCs), were observed. About 100 mL/L to 160 mL/L of the PEG-treated non-adherent cells with fluorescences were considered to be dendritomas that highly expressed the key molecules for antigen presentation in our five cases. In vitro studies showed that fusions effectively activated CD8(+) T lymphocytes to secrete interferon-gamma. The early apoptotic ratio of the colon cancer SW480 cells was higher than that of controls, which was affected by cytotoxic T lymphocytes (CTLs) stimulated by dendritomas.

CONCLUSION: The data indicate that fusion of tumor cells with DCs is an attractive strategy to induce tumor rejection.

语种: 英语
项目资助者: Technology Foundation of Ministry of Education, China
WOS记录号: WOS:000208097600017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65057
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Div Surg Oncol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Div Gastrointestinal Surg, Beijing 100044, Peoples R China

Recommended Citation:
Xu, Feng,Ye, Ying-Jiang,Wang, Shan. In vitro antitumor immune response induced by fusion of dendritic cells and colon cancer cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2004,10(8):1162-1166.
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