IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
In vitro antitumor immune response induced by fusion of dendritic cells and colon cancer cells
Xu, Feng1,2; Ye, Ying-Jiang1,2; Wang, Shan1,2
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2004-04-15
10期:8页:1162-1166
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
英文摘要

AIM: The prevention of recurrence of colon cancer (CC) after operation is very important for improvement of the prognosis of CC patients, especially those with micro-metastasis. The generation of fused cells between dendritic cells (DCs) and tumor cells maybe an effective approach for tumor antigen presentation in immunotherapy. In this study, we fused human colon caner SW480 cells and human peripheral blood - derived DCs to induce an antitumor activity against human CC.

METHODS: CC SW480 cells and human peripheral blood derived DCs were fused with 500 mL/L polyethylene glycol (PEG).

RESULTS: The specific T cell responses activated by fusion cells (FCs), were observed. About 100 mL/L to 160 mL/L of the PEG-treated non-adherent cells with fluorescences were considered to be dendritomas that highly expressed the key molecules for antigen presentation in our five cases. In vitro studies showed that fusions effectively activated CD8(+) T lymphocytes to secrete interferon-gamma. The early apoptotic ratio of the colon cancer SW480 cells was higher than that of controls, which was affected by cytotoxic T lymphocytes (CTLs) stimulated by dendritomas.

CONCLUSION: The data indicate that fusion of tumor cells with DCs is an attractive strategy to induce tumor rejection.

语种英语
WOS记录号WOS:000208097600017
资助机构Technology Foundation of Ministry of Education, China
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65057
专题北京大学第二临床医学院
北京大学药学院_天然药物学系
作者单位1.Peking Univ, Peoples Hosp, Div Surg Oncol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Div Gastrointestinal Surg, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Xu, Feng,Ye, Ying-Jiang,Wang, Shan. In vitro antitumor immune response induced by fusion of dendritic cells and colon cancer cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2004,10(8):1162-1166.
APA Xu, Feng,Ye, Ying-Jiang,&Wang, Shan.(2004).In vitro antitumor immune response induced by fusion of dendritic cells and colon cancer cells.WORLD JOURNAL OF GASTROENTEROLOGY,10(8),1162-1166.
MLA Xu, Feng,et al."In vitro antitumor immune response induced by fusion of dendritic cells and colon cancer cells".WORLD JOURNAL OF GASTROENTEROLOGY 10.8(2004):1162-1166.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xu, Feng]的文章
[Ye, Ying-Jiang]的文章
[Wang, Shan]的文章
百度学术
百度学术中相似的文章
[Xu, Feng]的文章
[Ye, Ying-Jiang]的文章
[Wang, Shan]的文章
必应学术
必应学术中相似的文章
[Xu, Feng]的文章
[Ye, Ying-Jiang]的文章
[Wang, Shan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。