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学科主题: 口腔医学
题名:
Molecular pathways involved in crosstalk between cancer cells, osteoblasts and osteoclasts in the invasion of bone by oral squamous cell carcinoma
作者: Quan, Jingjing2,3; Zhou, Chuanxiang8; Johnson, Newell W.4; Francis, Glenn5; Dahlstrom, Jane E.6,7; Gao, Jin1,2,3
关键词: Bone invasion ; immunohistochemistry ; MMPs ; OPG ; oral squamous cell carcinoma ; osteoblasts ; osteoclasts ; RANKL ; Runx2 ; Twist1
刊名: PATHOLOGY
发表日期: 2012-04-01
DOI: 10.1097/PAT.0b013e3283513f3b
卷: 44, 期:3, 页:221-227
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pathology
研究领域[WOS]: Pathology
关键词[WOS]: EPITHELIAL-MESENCHYMAL TRANSITION ; MATRIX METALLOPROTEINASES ; MANDIBULAR INVASION ; PROSTATE-CANCER ; DIFFERENTIATION ; METASTASIS ; MATRIX-METALLOPROTEINASE-9 ; INTERLEUKIN-6 ; MECHANISMS ; EXPRESSION
英文摘要:

Aims: This study investigates whether matrix metalloproteinases (MMPs), specifically MMP-2 and MMP-9, interacting with other molecules important in osteoblast differentiation and osteoclastogenesis, could play important roles in the invasion of bone by oral squamous cell carcinoma (OSCC).

Methods: Supernatant (conditioned medium, CM) was collected from OSCC cell lines (SCC15 and SCC25), and from cultured osteoblasts (hFOB cell line and a primary culture, OB), and used for indirect co-culture: OSCC cells were treated with CM from osteoblasts and vice versa. Zymogenic activities of MMP-2 and -9, and protein quantities of all molecules studied, were detected by gelatine zymography and Western blotting, respectively. Real-time polymerase chain reaction (PCR) analysed mRNA of these molecules. Targeted molecules were examined by immunohistochemistry in tissue sections of bone-invasive OSCCs.

Results: Zymogenic activities of both MMPs were increased in OSCC cells following culture with CM from hFOB: Twist1 protein expression was increased while Runx2 did not alter. The RANKL/OPG ratio, zymogen and protein expression of MMP-9 were increased in hFOB cells cultured with CM from OSCC lines, while zymogen expression of MMP-2 was decreased. Real-time PCR showed generally similar changes in expression of these molecules. All targeted molecules were expressed in invading malignant keratinocytes, and all but OPG were expressed in osteoclasts of clinical samples.

Conclusions: Crosstalk between different cell types appears to exist in the invasion of bone by OSCC. Understanding and ultimately interfering with the molecules involved may provide therapeutic approaches to inhibit such bone invasion.

语种: 英语
所属项目编号: CSC2008638008
项目资助者: China Scholarship Council ; Griffith University International Postgraduate Research Scholarship (GUIPRS) ; National Health and Medical Research Council (NHMRC) Australia ; Australian Dental Research Foundation (ADRF)
WOS记录号: WOS:000303635700008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65067
Appears in Collections:北京大学口腔医学院_口腔病理科_期刊论文

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作者单位: 1.Griffith Univ, Griffith Hlth Inst, Nathan, Qld 4111, Australia
2.Royal Brisbane & Women Hosp, Herston, Qld, Australia
3.James Cook Univ, Sch Med & Dent, Cairns, Qld 4870, Australia
4.Griffith Univ, Sch Dent, Nathan, Qld 4111, Australia
5.Griffith Univ, Sch Med Sci, Nathan, Qld 4111, Australia
6.Canberra Hosp, ACT Pathol, Dept Anat Pathol, Canberra, ACT, Australia
7.Australian Natl Univ, Sch Med, Canberra, ACT 0200, Australia
8.Peking Univ, Sch & Hosp Stomatol, Dept Oral Pathol, Beijing 100871, Peoples R China

Recommended Citation:
Quan, Jingjing,Zhou, Chuanxiang,Johnson, Newell W.,et al. Molecular pathways involved in crosstalk between cancer cells, osteoblasts and osteoclasts in the invasion of bone by oral squamous cell carcinoma[J]. PATHOLOGY,2012,44(3):221-227.
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