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学科主题临床医学
A Modified Method for Implantation of Pluripotent Stem Cells Under the Rodent Kidney Capsule
Zhu, Fuli1,2; Sun, Bin1,3; Wen, Yan1; Wang, Zhe1,4; Pera, Renee Reijo1,5; Chen, Bertha1
刊名STEM CELLS AND DEVELOPMENT
2014-09-01
DOI10.1089/scd.2014.0099
23期:17页:2119-2125
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Hematology ; Medicine, Research & Experimental ; Transplantation
研究领域[WOS]Cell Biology ; Hematology ; Research & Experimental Medicine ; Transplantation
关键词[WOS]TERATOMA FORMATION ; SAFETY
英文摘要

Teratoma formation, the standard in vivo pluripotency assay, is also frequently used as a tumorigenicity assay. A common concern in therapeutic stem cell applications is the tumorigenicity potential of a small number of cell impurities in the final product. Estimation of this small number is hampered by the inaccurate methodology of the tumorigenicity assay. Hence, a protocol for tumorigenicity assay that can deliver a defined number of cells, without error introduced by leakage or migration of cells is needed. In this study, we tested our modified transplantation method that allows for transplant of small numbers of pluripotent stem cells (PSCs) under the kidney capsule with minimal cell leakage. A glass capillary with a finely shaped tip and an attached mouth pipette was used to inject PSCs into the rodent kidney capsule. H9 embryonic and induced PSCs were tagged with Fluc and green fluorescence protein reporter genes and divided in different cell doses for transplantation. Bioluminescence imaging (BLI) on the day of surgery showed that the cell signal was confined to the kidney and signal intensity correlated with increasing transplant cell numbers. The overall cell leakage rate was 17% and the rodent survival rate was 96%. Teratoma formation was observed in rodents transplanted with cell numbers between 1x10(5)-2 x 10(6). We conclude that this modified procedure for transplanting PSCs under the kidney capsule allows for transplantation of a defined number of PSCs with significant reduction of error associated with cell leakage from the transplant site.

语种英语
WOS记录号WOS:000341017900011
项目编号106180-TR3-05569
资助机构CIRM (California Institute of Regenerative Medicine)
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65072
专题北京大学第三临床医学院
作者单位1.Guangzhou Med Univ, Guangzhou, Guangdong, Peoples R China
2.Stanford Univ, Sch Med, Dept Obstet Gynecol, Stanford, CA 94305 USA
3.Beijing Univ, Hosp 3, Dept Obstet Gynecol, Beijing 100871, Peoples R China
4.Southern Med Univ, Guangzhou, Guangdong, Peoples R China
5.Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA
推荐引用方式
GB/T 7714
Zhu, Fuli,Sun, Bin,Wen, Yan,et al. A Modified Method for Implantation of Pluripotent Stem Cells Under the Rodent Kidney Capsule[J]. STEM CELLS AND DEVELOPMENT,2014,23(17):2119-2125.
APA Zhu, Fuli,Sun, Bin,Wen, Yan,Wang, Zhe,Pera, Renee Reijo,&Chen, Bertha.(2014).A Modified Method for Implantation of Pluripotent Stem Cells Under the Rodent Kidney Capsule.STEM CELLS AND DEVELOPMENT,23(17),2119-2125.
MLA Zhu, Fuli,et al."A Modified Method for Implantation of Pluripotent Stem Cells Under the Rodent Kidney Capsule".STEM CELLS AND DEVELOPMENT 23.17(2014):2119-2125.
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