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学科主题: 临床医学
题名:
A Modified Method for Implantation of Pluripotent Stem Cells Under the Rodent Kidney Capsule
作者: Zhu, Fuli1,2; Sun, Bin1,3; Wen, Yan1; Wang, Zhe1,4; Pera, Renee Reijo1,5; Chen, Bertha1
刊名: STEM CELLS AND DEVELOPMENT
发表日期: 2014-09-01
DOI: 10.1089/scd.2014.0099
卷: 23, 期:17, 页:2119-2125
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Hematology ; Medicine, Research & Experimental ; Transplantation
研究领域[WOS]: Cell Biology ; Hematology ; Research & Experimental Medicine ; Transplantation
关键词[WOS]: TERATOMA FORMATION ; SAFETY
英文摘要:

Teratoma formation, the standard in vivo pluripotency assay, is also frequently used as a tumorigenicity assay. A common concern in therapeutic stem cell applications is the tumorigenicity potential of a small number of cell impurities in the final product. Estimation of this small number is hampered by the inaccurate methodology of the tumorigenicity assay. Hence, a protocol for tumorigenicity assay that can deliver a defined number of cells, without error introduced by leakage or migration of cells is needed. In this study, we tested our modified transplantation method that allows for transplant of small numbers of pluripotent stem cells (PSCs) under the kidney capsule with minimal cell leakage. A glass capillary with a finely shaped tip and an attached mouth pipette was used to inject PSCs into the rodent kidney capsule. H9 embryonic and induced PSCs were tagged with Fluc and green fluorescence protein reporter genes and divided in different cell doses for transplantation. Bioluminescence imaging (BLI) on the day of surgery showed that the cell signal was confined to the kidney and signal intensity correlated with increasing transplant cell numbers. The overall cell leakage rate was 17% and the rodent survival rate was 96%. Teratoma formation was observed in rodents transplanted with cell numbers between 1x10(5)-2 x 10(6). We conclude that this modified procedure for transplanting PSCs under the kidney capsule allows for transplantation of a defined number of PSCs with significant reduction of error associated with cell leakage from the transplant site.

语种: 英语
所属项目编号: 106180-TR3-05569
项目资助者: CIRM (California Institute of Regenerative Medicine)
WOS记录号: WOS:000341017900011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65072
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Guangzhou Med Univ, Guangzhou, Guangdong, Peoples R China
2.Stanford Univ, Sch Med, Dept Obstet Gynecol, Stanford, CA 94305 USA
3.Beijing Univ, Hosp 3, Dept Obstet Gynecol, Beijing 100871, Peoples R China
4.Southern Med Univ, Guangzhou, Guangdong, Peoples R China
5.Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA

Recommended Citation:
Zhu, Fuli,Sun, Bin,Wen, Yan,et al. A Modified Method for Implantation of Pluripotent Stem Cells Under the Rodent Kidney Capsule[J]. STEM CELLS AND DEVELOPMENT,2014,23(17):2119-2125.
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