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Dynamic pattern of gene expression of ZnT-1, ZnT-3 and PRG-1 in rat brain following flurothyl-induced recurrent neonatal seizures
Ni, Hong1,2; Jiang, Yu-wu3; Xiao, Zhuo-jun1,2; Tao, Lu-yang1,2; Jin, Mei-fang1,2; Wu, Xi-ru3
关键词Zinc transporter 1 Zinc transporter 3 PRG-1 Developmental Seizure
刊名TOXICOLOGY LETTERS
2010-05-04
DOI10.1016/j.toxlet.2010.02.008
194期:3页:86-93
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Toxicology
研究领域[WOS]Toxicology
关键词[WOS]ENTORHINAL CORTEX ; DENTATE GYRUS ; COGNITIVE DEFICITS ; LIPID-PHOSPHATASE ; NEURONAL INJURY ; MOSSY FIBERS ; KAINIC ACID ; ZINC ; HIPPOCAMPUS ; PLASTICITY
英文摘要

Zinc transporters (ZnTs) and plasticity-related genes (PRGs) both play the key roles in the formation of hippocampal mossy fiber sprouting, which is associated with cognitive deficits following developmental seizures. Here, for the first time, we report the timing of expression pattern of ZnT-1, ZnT-3 and PRG-1 in hippocampus and cerebral cortex following developmental seizures. A seizure was induced by inhalant flurothyl daily in neonatal Sprague-Dawley rats from postnatal day 6 (P6). Rats were assigned into the recurrent-seizure group (RS, seizures induced in 6 consecutive days) and the control group. At 1.5 h, 3h, 6h, 12h, 24h, 48h, 7d and 14d after the last seizures, the mRNA level was detected using RT-PCR method; PRG-1 protein level was examined by Western blotting analysis. At an early period of 12 h and 48 h after the last seizures, both ZnT-1 and ZnT-3 showed significantly down-regulated mRNA level in the cerebral cortex of RS group than those at the corresponding time point in control group. In the long-term time point of 14d after the last seizure, ZnT-3 mRNA and PRG-1 protein level in hippocampus were up-regulated while the mRNA level of ZnT-1 down-regulated; in addition, there were up-regulated level of both the mRNA and protein level of PRG-1 and down-regulated mRNA level of ZnT-3 in the cerebral cortex of RS group when compared to the control. Taken together, these dates are consistent with an important role for ZnT-1, ZnT-3 and PRG-1 in the pathophysiology of the long-term adverse effects of recurrent neonatal seizure-induced hippocampal mossy fiber sprouting and cognitive deficit. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000276933500006
项目编号30470555 ; 30870808 ; 30872666 ; 2003034094 ; BK2007509 ; 07KJB320103
资助机构National Natural Science Foundation of China ; 34th Postdoctoral Science Foundation of China ; Natural Science Foundation of Jiangsu Province of China
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65073
专题北京大学第一临床医学院_儿科
作者单位1.Soochow Univ, Neurol Lab, Children Hosp Affiliated, Suzhou 215003, Peoples R China
2.Soochow Univ, Lab Aging & Nervous Dis, Suzhou 215003, Peoples R China
3.Peking Univ, Affiliated Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
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GB/T 7714
Ni, Hong,Jiang, Yu-wu,Xiao, Zhuo-jun,et al. Dynamic pattern of gene expression of ZnT-1, ZnT-3 and PRG-1 in rat brain following flurothyl-induced recurrent neonatal seizures[J]. TOXICOLOGY LETTERS,2010,194(3):86-93.
APA Ni, Hong,Jiang, Yu-wu,Xiao, Zhuo-jun,Tao, Lu-yang,Jin, Mei-fang,&Wu, Xi-ru.(2010).Dynamic pattern of gene expression of ZnT-1, ZnT-3 and PRG-1 in rat brain following flurothyl-induced recurrent neonatal seizures.TOXICOLOGY LETTERS,194(3),86-93.
MLA Ni, Hong,et al."Dynamic pattern of gene expression of ZnT-1, ZnT-3 and PRG-1 in rat brain following flurothyl-induced recurrent neonatal seizures".TOXICOLOGY LETTERS 194.3(2010):86-93.
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