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学科主题: 临床医学
题名:
Dynamic pattern of gene expression of ZnT-1, ZnT-3 and PRG-1 in rat brain following flurothyl-induced recurrent neonatal seizures
作者: Ni, Hong1,2; Jiang, Yu-wu3; Xiao, Zhuo-jun1,2; Tao, Lu-yang1,2; Jin, Mei-fang1,2; Wu, Xi-ru3
关键词: Zinc transporter 1 ; Zinc transporter 3 ; PRG-1 ; Developmental ; Seizure
刊名: TOXICOLOGY LETTERS
发表日期: 2010-05-04
DOI: 10.1016/j.toxlet.2010.02.008
卷: 194, 期:3, 页:86-93
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: ENTORHINAL CORTEX ; DENTATE GYRUS ; COGNITIVE DEFICITS ; LIPID-PHOSPHATASE ; NEURONAL INJURY ; MOSSY FIBERS ; KAINIC ACID ; ZINC ; HIPPOCAMPUS ; PLASTICITY
英文摘要:

Zinc transporters (ZnTs) and plasticity-related genes (PRGs) both play the key roles in the formation of hippocampal mossy fiber sprouting, which is associated with cognitive deficits following developmental seizures. Here, for the first time, we report the timing of expression pattern of ZnT-1, ZnT-3 and PRG-1 in hippocampus and cerebral cortex following developmental seizures. A seizure was induced by inhalant flurothyl daily in neonatal Sprague-Dawley rats from postnatal day 6 (P6). Rats were assigned into the recurrent-seizure group (RS, seizures induced in 6 consecutive days) and the control group. At 1.5 h, 3h, 6h, 12h, 24h, 48h, 7d and 14d after the last seizures, the mRNA level was detected using RT-PCR method; PRG-1 protein level was examined by Western blotting analysis. At an early period of 12 h and 48 h after the last seizures, both ZnT-1 and ZnT-3 showed significantly down-regulated mRNA level in the cerebral cortex of RS group than those at the corresponding time point in control group. In the long-term time point of 14d after the last seizure, ZnT-3 mRNA and PRG-1 protein level in hippocampus were up-regulated while the mRNA level of ZnT-1 down-regulated; in addition, there were up-regulated level of both the mRNA and protein level of PRG-1 and down-regulated mRNA level of ZnT-3 in the cerebral cortex of RS group when compared to the control. Taken together, these dates are consistent with an important role for ZnT-1, ZnT-3 and PRG-1 in the pathophysiology of the long-term adverse effects of recurrent neonatal seizure-induced hippocampal mossy fiber sprouting and cognitive deficit. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 30470555 ; 30870808 ; 30872666 ; 2003034094 ; BK2007509 ; 07KJB320103
项目资助者: National Natural Science Foundation of China ; 34th Postdoctoral Science Foundation of China ; Natural Science Foundation of Jiangsu Province of China
WOS记录号: WOS:000276933500006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65073
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Soochow Univ, Neurol Lab, Children Hosp Affiliated, Suzhou 215003, Peoples R China
2.Soochow Univ, Lab Aging & Nervous Dis, Suzhou 215003, Peoples R China
3.Peking Univ, Affiliated Hosp 1, Dept Pediat, Beijing 100034, Peoples R China

Recommended Citation:
Ni, Hong,Jiang, Yu-wu,Xiao, Zhuo-jun,et al. Dynamic pattern of gene expression of ZnT-1, ZnT-3 and PRG-1 in rat brain following flurothyl-induced recurrent neonatal seizures[J]. TOXICOLOGY LETTERS,2010,194(3):86-93.
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