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Changes in histone methylation during human oocyte maturation and IVF- or ICSI-derived embryo development
Qiao, Jie1; Chen, Yuan1; Yan, Li-Ying1; Yan, Jie1; Liu, Ping1; Sun, Qing-Yuan2
关键词Histone methylation oocyte tripronuclear zygote human embryo
刊名FERTILITY AND STERILITY
2010-03-15
DOI10.1016/j.fertnstert.2009.03.002
93期:5页:1628-1636
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
资助者Major State Basic Research Development Program of China ; Major State Basic Research Development Program of China
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]IN-VITRO FERTILIZATION ; INTRACYTOPLASMIC SPERM INJECTION ; DNA METHYLATION ; HUMAN ZYGOTES ; PREIMPLANTATION EMBRYOS ; LYSINE METHYLATION ; MOUSE EMBRYOS ; CHROMATIN ; PATTERNS ; VIVO
英文摘要

Objective: To characterize the histone methylation pattern during human oocyte maturation and embryo development after conventional IVF and ICSI.

Design: Experimental study. Setting: Reproductive center of hospital.

Patient(s): Women underwent IVF or ICSI.

Intervention(s): Immature and mature oocytes were collected from patients undergoing ICSI. Tripronuclear and normally fertilized embryos were obtained from patients undergoing IVF or ICSI.

Main Outcome Measure(s): The distribution patterns of dimethylated histone H3 lysine 9 (H3K9) and histone H4 arginine 3 (H4R3) in oocytes and embryos were observed by indirect immunofluorescent staining and scanning confocal microscopy.

Result(s): H3K9 and H4R3 were dimethylated throughout the meiotic maturation of human oocytes and the tripronuclear embryo development from two-cell to blastocyst stage. However, at the pronuclear stage, approximately half of the tripronuclear IVF zygotes displayed strong staining of MeH3K9 in one pronucleus, whereas MeH4R3 staining was always uniform in all three pronuclei. In the other half of the tripronuclear zygotes, all three pronuclei were strongly stained with MeH3K9 in some cases, and the remaining zygotes were completely unstained. Moreover, with progressively increasing fragmentation of blastomeres in ICSI-derived 2PN embryos with low morphological grade. H3K9 dimethylation decreased when compared with that of IVF embryos.

Conclusion(s): Asymmetric distribution of the dimethylation form of H3K9 exists in human zygote pronuclei. The ICSI-derived embryos with low morphological grade are more likely to display H3K9 demethylation than their IVF counterparts. (Fertil Steril (R) 2010;93:1628-36. (C)2010 by American Society for Reproductive Medicine.)

语种英语
所属项目编号2007CB948102
资助者Major State Basic Research Development Program of China ; Major State Basic Research Development Program of China
WOS记录号WOS:000276038700032
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65083
专题北京大学第三临床医学院_生殖医学中心
作者单位1.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Qiao, Jie,Chen, Yuan,Yan, Li-Ying,et al. Changes in histone methylation during human oocyte maturation and IVF- or ICSI-derived embryo development[J]. FERTILITY AND STERILITY,2010,93(5):1628-1636.
APA Qiao, Jie,Chen, Yuan,Yan, Li-Ying,Yan, Jie,Liu, Ping,&Sun, Qing-Yuan.(2010).Changes in histone methylation during human oocyte maturation and IVF- or ICSI-derived embryo development.FERTILITY AND STERILITY,93(5),1628-1636.
MLA Qiao, Jie,et al."Changes in histone methylation during human oocyte maturation and IVF- or ICSI-derived embryo development".FERTILITY AND STERILITY 93.5(2010):1628-1636.
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