IR@PKUHSC  > 北京大学第三临床医学院  > 皮肤科
学科主题临床医学
Effects of Cigarette Smoke Extracts on the Growth and Senescence of Skin Fibroblasts In Vitro
Yang, Gao-yun1; Zhang, Chun-lei2; Liu, Xiang-chen1; Qian, Ge3; Deng, Dan-qi3
关键词skin fibroblasts senescence growth cigarette smoke extract senescence-associated beta-galactosidase
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
2013
DOI10.7150/ijbs.6162
9期:6页:613-623
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
关键词[WOS]BETA-GALACTOSIDASE ; CELLULAR SENESCENCE ; ULTRAVIOLET-LIGHT ; HUMAN-CELLS ; APOPTOSIS ; EXPOSURE ; EXPRESSION ; BIOMARKER ; RESPONSES ; PATHWAY
英文摘要

Epidemiological studies have shown that cigarette smoke (CS), a very common environmental factor, plays an important role in skin aging. Although some in vivo studies have suggested that CS affects skin aging, the detailed effects of CS on skin cells in vitro remain largely unknown. In this study, we investigated the effects of cigarette smoke extract (CSE) on the growth, proliferation, and senescene of skin fibroblasts and the possible mechanism underlying these effects. Primary cultured human fibroblasts were exposed to a range of concentrations of CSE. Cell viability and cell proliferation after CSE exposure were analyzed with the methyl thiazolyl tetrazolium (MTT) assay and bromodeoxyuridine incorporation assay, respectively. Growth curves of fibroblasts exposed to different concentrations of CSE were developed and prolonged CSE-exposed cells were observed. Morphological and ultrastructural changes in fibroblasts were assessed by inverted light microscopy and transmission electron microscopy (TEM). Dying cells were stained with senescence-associated beta-galactosidase (SA beta-gal). Intracellular reactive oxygen species (ROS) levels, superoxide dismutase (SOD) activity, and glutathione peroxidase (GSH-Px) activity were determined by a colorimetric method. We found that proliferative capacity and growth were inhibited by CSE exposure in a dose- and time-dependent manner. Fibroblasts exposed to even low concentrations of CSE for a long period of time (5 passages) showed significantly increased SA beta-gal activity and typical features of aging cells. Meanwhile, CSE inhibited superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and augmented ROS levels. Our observations suggest that CSE exposure impairs fibroblast growth and proliferation and leads to features similar to those seen in senescent cells. Oxidative stress injury and inhibition of antioxidant defense activity may be involved in CSE-induced fibroblast senescence.

语种英语
WOS记录号WOS:000321284800009
资助机构Chinese Medical Association (L&prime ; OREAL CHINA SKIN GRANT)
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65092
专题北京大学第三临床医学院_皮肤科
作者单位1.Capital Med Univ, Dept Dermatol, Beijing Friendship Hosp, Beijing, Peoples R China
2.Peking Univ, Dept Dermatol, Hosp 3, Beijing 100871, Peoples R China
3.Kunming Med Univ, Dept Dermatol & Rheumatol, Affiliated Hosp 2, Kunming 650101, Peoples R China
推荐引用方式
GB/T 7714
Yang, Gao-yun,Zhang, Chun-lei,Liu, Xiang-chen,et al. Effects of Cigarette Smoke Extracts on the Growth and Senescence of Skin Fibroblasts In Vitro[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2013,9(6):613-623.
APA Yang, Gao-yun,Zhang, Chun-lei,Liu, Xiang-chen,Qian, Ge,&Deng, Dan-qi.(2013).Effects of Cigarette Smoke Extracts on the Growth and Senescence of Skin Fibroblasts In Vitro.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,9(6),613-623.
MLA Yang, Gao-yun,et al."Effects of Cigarette Smoke Extracts on the Growth and Senescence of Skin Fibroblasts In Vitro".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 9.6(2013):613-623.
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