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IR@PKUHSC  > 北京大学第三临床医学院  > 皮肤科  > 期刊论文
学科主题: 临床医学
题名:
Effects of Cigarette Smoke Extracts on the Growth and Senescence of Skin Fibroblasts In Vitro
作者: Yang, Gao-yun1; Zhang, Chun-lei2; Liu, Xiang-chen1; Qian, Ge3; Deng, Dan-qi3
关键词: skin fibroblasts ; senescence ; growth ; cigarette smoke extract ; senescence-associated beta-galactosidase
刊名: INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
发表日期: 2013
DOI: 10.7150/ijbs.6162
卷: 9, 期:6, 页:613-623
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
关键词[WOS]: BETA-GALACTOSIDASE ; CELLULAR SENESCENCE ; ULTRAVIOLET-LIGHT ; HUMAN-CELLS ; APOPTOSIS ; EXPOSURE ; EXPRESSION ; BIOMARKER ; RESPONSES ; PATHWAY
英文摘要:

Epidemiological studies have shown that cigarette smoke (CS), a very common environmental factor, plays an important role in skin aging. Although some in vivo studies have suggested that CS affects skin aging, the detailed effects of CS on skin cells in vitro remain largely unknown. In this study, we investigated the effects of cigarette smoke extract (CSE) on the growth, proliferation, and senescene of skin fibroblasts and the possible mechanism underlying these effects. Primary cultured human fibroblasts were exposed to a range of concentrations of CSE. Cell viability and cell proliferation after CSE exposure were analyzed with the methyl thiazolyl tetrazolium (MTT) assay and bromodeoxyuridine incorporation assay, respectively. Growth curves of fibroblasts exposed to different concentrations of CSE were developed and prolonged CSE-exposed cells were observed. Morphological and ultrastructural changes in fibroblasts were assessed by inverted light microscopy and transmission electron microscopy (TEM). Dying cells were stained with senescence-associated beta-galactosidase (SA beta-gal). Intracellular reactive oxygen species (ROS) levels, superoxide dismutase (SOD) activity, and glutathione peroxidase (GSH-Px) activity were determined by a colorimetric method. We found that proliferative capacity and growth were inhibited by CSE exposure in a dose- and time-dependent manner. Fibroblasts exposed to even low concentrations of CSE for a long period of time (5 passages) showed significantly increased SA beta-gal activity and typical features of aging cells. Meanwhile, CSE inhibited superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and augmented ROS levels. Our observations suggest that CSE exposure impairs fibroblast growth and proliferation and leads to features similar to those seen in senescent cells. Oxidative stress injury and inhibition of antioxidant defense activity may be involved in CSE-induced fibroblast senescence.

语种: 英语
项目资助者: Chinese Medical Association (L&prime ; OREAL CHINA SKIN GRANT)
WOS记录号: WOS:000321284800009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65092
Appears in Collections:北京大学第三临床医学院_皮肤科_期刊论文

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作者单位: 1.Capital Med Univ, Dept Dermatol, Beijing Friendship Hosp, Beijing, Peoples R China
2.Peking Univ, Dept Dermatol, Hosp 3, Beijing 100871, Peoples R China
3.Kunming Med Univ, Dept Dermatol & Rheumatol, Affiliated Hosp 2, Kunming 650101, Peoples R China

Recommended Citation:
Yang, Gao-yun,Zhang, Chun-lei,Liu, Xiang-chen,et al. Effects of Cigarette Smoke Extracts on the Growth and Senescence of Skin Fibroblasts In Vitro[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2013,9(6):613-623.
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