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学科主题: 基础医学
题名:
Ganoderma lucidum polysaccharides antagonize the suppression on lymphocytes induced by culture supernatants of B16F10 melanoma cells
作者: Sun, Li-Xin1,2; Lin, Zhi-Bin1; Duan, Xin-Suo2; Lu, Jie2; Ge, Zhi-Hua2; Li, Xue-Jun1; Li, Min1; Xing, En-Hong2; Jia, Jing2; Lan, Tian-Fei2; Li, Wei-Dong1
关键词: Ganoderma lucidum polysaccharides ; granzyme B ; lymphocyte ; perforin ; tumour
刊名: JOURNAL OF PHARMACY AND PHARMACOLOGY
发表日期: 2011-05-01
DOI: 10.1111/j.2042-7158.2011.01266.x
卷: 63, 期:5, 页:725-735
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: GROWTH-FACTOR-BETA ; TGF-BETA ; T-CELLS ; IMMUNE SUPPRESSION ; COLORECTAL-CANCER ; DOWN-REGULATION ; SERUM INTERLEUKIN-10 ; CYTOKINE PRODUCTION ; ANTITUMOR IMMUNITY ; LANGERHANS CELLS
英文摘要:

Objectives

Tumour cells produce factors such as interleukin 10 (IL-10), transforming growth factor beta 1 (TGF-beta 1) and vascular endothelial growth factor (VEGF) that suppress the function of immune cells or induce apoptosis of immune cells. One of the most important goals of tumour immunotherapy is to antagonize this suppression on immune cells. Ganoderma lucidum polysaccharides (Gl-PS) may have this potential. The purpose of this study was to determine the antagonistic effects of Gl-PS on the suppression induced by B16F10 melanoma cell culture supernatant (B16F10-CS) on lymphocytes.

Methods

Gl-PS was used on lymphocytes incubated with B16F10-CS. Enzyme-linked immunosorbent assay was used to determine the levels of IL-10, TGF-beta 1 and VEGF in B16F10-CS. The MTT assay was used to determine the proliferation of lymphocytes. Immunocytochemistry and Western blot assay were used to determine perforin and granzyme B production in lymphocytes.

Key findings

There were elevated levels of IL-10, TGF-beta 1 and VEGF in B16F10-CS. The lymphocyte proliferation, and perforin and granzyme B production in lymphocytes after induction with phytohemagglutinin, as well as lymphocyte proliferation in the mixed lymphocyte reaction, were suppressed by B16F10-CS. This suppression was fully or partially antagonized by Gl-PS.

Conclusions

B16F10-CS suppressed lymphocyte proliferation and perforin and granzyme B production in lymphocytes after induction with phytohemagglutinin, as well as lymphocyte proliferation in the mixed lymphocyte reaction. This suppression may be associated with elevated levels of immunosuppressive IL-10, TGF-beta 1 and VEGF in B16F10-CS. Gl-PS had antagonistic effects on the immunosuppression induced by B16F10-CS, suggesting the potential for Gl-PS in cancer immunotherapy.

语种: 英语
WOS记录号: WOS:000289524800015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65097
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Peking Univ, Dept Pharmacol, Hlth Sci Ctr, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Chengde Med Coll, Affiliated Hosp, Chengde, Hebei Province, Peoples R China

Recommended Citation:
Sun, Li-Xin,Lin, Zhi-Bin,Duan, Xin-Suo,et al. Ganoderma lucidum polysaccharides antagonize the suppression on lymphocytes induced by culture supernatants of B16F10 melanoma cells[J]. JOURNAL OF PHARMACY AND PHARMACOLOGY,2011,63(5):725-735.
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