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学科主题基础医学
Ganoderma lucidum polysaccharides antagonize the suppression on lymphocytes induced by culture supernatants of B16F10 melanoma cells
Sun, Li-Xin1,2; Lin, Zhi-Bin1; Duan, Xin-Suo2; Lu, Jie2; Ge, Zhi-Hua2; Li, Xue-Jun1; Li, Min1; Xing, En-Hong2; Jia, Jing2; Lan, Tian-Fei2; Li, Wei-Dong1
关键词Ganoderma lucidum polysaccharides granzyme B lymphocyte perforin tumour
刊名JOURNAL OF PHARMACY AND PHARMACOLOGY
2011-05-01
DOI10.1111/j.2042-7158.2011.01266.x
63期:5页:725-735
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]GROWTH-FACTOR-BETA ; TGF-BETA ; T-CELLS ; IMMUNE SUPPRESSION ; COLORECTAL-CANCER ; DOWN-REGULATION ; SERUM INTERLEUKIN-10 ; CYTOKINE PRODUCTION ; ANTITUMOR IMMUNITY ; LANGERHANS CELLS
英文摘要

Objectives

Tumour cells produce factors such as interleukin 10 (IL-10), transforming growth factor beta 1 (TGF-beta 1) and vascular endothelial growth factor (VEGF) that suppress the function of immune cells or induce apoptosis of immune cells. One of the most important goals of tumour immunotherapy is to antagonize this suppression on immune cells. Ganoderma lucidum polysaccharides (Gl-PS) may have this potential. The purpose of this study was to determine the antagonistic effects of Gl-PS on the suppression induced by B16F10 melanoma cell culture supernatant (B16F10-CS) on lymphocytes.

Methods

Gl-PS was used on lymphocytes incubated with B16F10-CS. Enzyme-linked immunosorbent assay was used to determine the levels of IL-10, TGF-beta 1 and VEGF in B16F10-CS. The MTT assay was used to determine the proliferation of lymphocytes. Immunocytochemistry and Western blot assay were used to determine perforin and granzyme B production in lymphocytes.

Key findings

There were elevated levels of IL-10, TGF-beta 1 and VEGF in B16F10-CS. The lymphocyte proliferation, and perforin and granzyme B production in lymphocytes after induction with phytohemagglutinin, as well as lymphocyte proliferation in the mixed lymphocyte reaction, were suppressed by B16F10-CS. This suppression was fully or partially antagonized by Gl-PS.

Conclusions

B16F10-CS suppressed lymphocyte proliferation and perforin and granzyme B production in lymphocytes after induction with phytohemagglutinin, as well as lymphocyte proliferation in the mixed lymphocyte reaction. This suppression may be associated with elevated levels of immunosuppressive IL-10, TGF-beta 1 and VEGF in B16F10-CS. Gl-PS had antagonistic effects on the immunosuppression induced by B16F10-CS, suggesting the potential for Gl-PS in cancer immunotherapy.

语种英语
WOS记录号WOS:000289524800015
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65097
专题基础医学院_药理学系
作者单位1.Peking Univ, Dept Pharmacol, Hlth Sci Ctr, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Chengde Med Coll, Affiliated Hosp, Chengde, Hebei Province, Peoples R China
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GB/T 7714
Sun, Li-Xin,Lin, Zhi-Bin,Duan, Xin-Suo,et al. Ganoderma lucidum polysaccharides antagonize the suppression on lymphocytes induced by culture supernatants of B16F10 melanoma cells[J]. JOURNAL OF PHARMACY AND PHARMACOLOGY,2011,63(5):725-735.
APA Sun, Li-Xin.,Lin, Zhi-Bin.,Duan, Xin-Suo.,Lu, Jie.,Ge, Zhi-Hua.,...&Li, Wei-Dong.(2011).Ganoderma lucidum polysaccharides antagonize the suppression on lymphocytes induced by culture supernatants of B16F10 melanoma cells.JOURNAL OF PHARMACY AND PHARMACOLOGY,63(5),725-735.
MLA Sun, Li-Xin,et al."Ganoderma lucidum polysaccharides antagonize the suppression on lymphocytes induced by culture supernatants of B16F10 melanoma cells".JOURNAL OF PHARMACY AND PHARMACOLOGY 63.5(2011):725-735.
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