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Newly identified cytoskeletal components are associated with dynamic changes of podocyte foot processes
Miao, Jing1; Fan, Qingfeng1; Cui, Qinghua2; Zhang, Han1; Chen, Lihong3; Wang, Suxia4; Guan, Na1; Guan, Youfei3; Ding, Jie1
关键词microarray analysis podocyte cytoskeleton proteinuria puromycin aminonucleoside nephropathy
刊名NEPHROLOGY DIALYSIS TRANSPLANTATION
2009-11-01
DOI10.1093/ndt/gfp338
24期:11页:3297-3305
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Transplantation ; Urology & Nephrology
研究领域[WOS]Transplantation ; Urology & Nephrology
关键词[WOS]FOCAL SEGMENTAL GLOMERULOSCLEROSIS ; SLIT DIAPHRAGM ; NEPHRIN ; PROTEIN ; GENE ; DISEASE ; KINASE ; DOMAIN ; BINDS ; RAT
英文摘要

Background. Proteinuria, one of the main manifestations of nephrotic syndrome, is an important risk factor for the progression of renal diseases. Podocyte foot processes (FPs) injury induces proteinuria in most renal diseases. The podocyte cytoskeleton plays important roles in maintaining the normal morphology of FPs. However, the underlying cytoskeletal component that initiates and regulates the dynamic changes of FPs is still unclear. Here, the involved podocyte cytoskeletal molecules were explored on different days in puromycin aminonucleoside nephropathy rats.

Methods. Microarray analysis of isolated glomeruli was performed at Day 2, Day 10 and Day 15 in puromycin aminonucleoside nephropathy rats. Cytoskeletal genebank was established by sorting with the keyword ′cytoskeleton′ from PUBMED genebank to identify the differential cytoskeleton genes. Microarray results were further confirmed by real-time PCR, western blot and double immunolabelling to validate their localizations.

Results. Nine different cytoskeletal genes were found to be involved in the dynamic changes of FPs in puromycin aminonucleoside nephropathy rats, including six up-regulated (Tagln, Actr2, Dnm3, Arc, Vcl and Birc5) and three down-regulated (Krt2-7, Nebl and Tnncl). The differential expression of transgelin, survivin, arp2, cytokeratin7 and vinculin was verified by real-time PCR and western blot. Double immunolabelling revealed that five cytoskeletal proteins indeed colocalized with podocyte specific markers synaptopodin or alpha-actinin-4. In addition, similar expression and distribution changes were detected in patients with proteinuric renal diseases and puromycin aminonucleoside-treated podocytes.

Conclusions. We identified five novel podocyte cytoskeletal proteins and found that they were associated with the dynamic changes of FPs in podocyte injury.

语种英语
WOS记录号WOS:000271718900012
项目编号30830105 ; 30170992 ; 30672259 ; 7072080
资助机构National Nature Science Foundation of China ; Nature Science Foundation of Beijing
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65107
专题北京大学第一临床医学院_儿科
北京大学基础医学院
北京大学第一临床医学院_电镜室
北京大学第二临床医学院_风湿免疫科
作者单位1.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
2.Peking Univ, Dept Med Informat, Hlth Sci Ctr, Beijing 100083, Peoples R China
3.Peking Univ, Dept Physiol & Pathophysiol, Hlth Sci Ctr, Beijing 100083, Peoples R China
4.Peking Univ, Hosp 1, Dept Electron Microscopy, Beijing 100034, Peoples R China
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GB/T 7714
Miao, Jing,Fan, Qingfeng,Cui, Qinghua,et al. Newly identified cytoskeletal components are associated with dynamic changes of podocyte foot processes[J]. NEPHROLOGY DIALYSIS TRANSPLANTATION,2009,24(11):3297-3305.
APA Miao, Jing.,Fan, Qingfeng.,Cui, Qinghua.,Zhang, Han.,Chen, Lihong.,...&Ding, Jie.(2009).Newly identified cytoskeletal components are associated with dynamic changes of podocyte foot processes.NEPHROLOGY DIALYSIS TRANSPLANTATION,24(11),3297-3305.
MLA Miao, Jing,et al."Newly identified cytoskeletal components are associated with dynamic changes of podocyte foot processes".NEPHROLOGY DIALYSIS TRANSPLANTATION 24.11(2009):3297-3305.
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