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学科主题: 临床医学
题名:
Newly identified cytoskeletal components are associated with dynamic changes of podocyte foot processes
作者: Miao, Jing1; Fan, Qingfeng1; Cui, Qinghua2; Zhang, Han1; Chen, Lihong3; Wang, Suxia4; Guan, Na1; Guan, Youfei3; Ding, Jie1
关键词: microarray analysis ; podocyte cytoskeleton ; proteinuria ; puromycin aminonucleoside nephropathy
刊名: NEPHROLOGY DIALYSIS TRANSPLANTATION
发表日期: 2009-11-01
DOI: 10.1093/ndt/gfp338
卷: 24, 期:11, 页:3297-3305
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Transplantation ; Urology & Nephrology
研究领域[WOS]: Transplantation ; Urology & Nephrology
关键词[WOS]: FOCAL SEGMENTAL GLOMERULOSCLEROSIS ; SLIT DIAPHRAGM ; NEPHRIN ; PROTEIN ; GENE ; DISEASE ; KINASE ; DOMAIN ; BINDS ; RAT
英文摘要:

Background. Proteinuria, one of the main manifestations of nephrotic syndrome, is an important risk factor for the progression of renal diseases. Podocyte foot processes (FPs) injury induces proteinuria in most renal diseases. The podocyte cytoskeleton plays important roles in maintaining the normal morphology of FPs. However, the underlying cytoskeletal component that initiates and regulates the dynamic changes of FPs is still unclear. Here, the involved podocyte cytoskeletal molecules were explored on different days in puromycin aminonucleoside nephropathy rats.

Methods. Microarray analysis of isolated glomeruli was performed at Day 2, Day 10 and Day 15 in puromycin aminonucleoside nephropathy rats. Cytoskeletal genebank was established by sorting with the keyword ′cytoskeleton′ from PUBMED genebank to identify the differential cytoskeleton genes. Microarray results were further confirmed by real-time PCR, western blot and double immunolabelling to validate their localizations.

Results. Nine different cytoskeletal genes were found to be involved in the dynamic changes of FPs in puromycin aminonucleoside nephropathy rats, including six up-regulated (Tagln, Actr2, Dnm3, Arc, Vcl and Birc5) and three down-regulated (Krt2-7, Nebl and Tnncl). The differential expression of transgelin, survivin, arp2, cytokeratin7 and vinculin was verified by real-time PCR and western blot. Double immunolabelling revealed that five cytoskeletal proteins indeed colocalized with podocyte specific markers synaptopodin or alpha-actinin-4. In addition, similar expression and distribution changes were detected in patients with proteinuric renal diseases and puromycin aminonucleoside-treated podocytes.

Conclusions. We identified five novel podocyte cytoskeletal proteins and found that they were associated with the dynamic changes of FPs in podocyte injury.

语种: 英语
所属项目编号: 30830105 ; 30170992 ; 30672259 ; 7072080
项目资助者: National Nature Science Foundation of China ; Nature Science Foundation of Beijing
WOS记录号: WOS:000271718900012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65107
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
2.Peking Univ, Dept Med Informat, Hlth Sci Ctr, Beijing 100083, Peoples R China
3.Peking Univ, Dept Physiol & Pathophysiol, Hlth Sci Ctr, Beijing 100083, Peoples R China
4.Peking Univ, Hosp 1, Dept Electron Microscopy, Beijing 100034, Peoples R China

Recommended Citation:
Miao, Jing,Fan, Qingfeng,Cui, Qinghua,et al. Newly identified cytoskeletal components are associated with dynamic changes of podocyte foot processes[J]. NEPHROLOGY DIALYSIS TRANSPLANTATION,2009,24(11):3297-3305.
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