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Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by oridonin nanosuspension
Feng, Fei-Fei1; Zhang, Dian-Rui1; Tian, Ke-Li2; Lou, Hai-Yan3; Qi, Xiao-Li2; Wang, Yan-Cai1; Duan, Cun-Xian1; Jia, Le-Jiao1; Wang, Fei-Hu1; Liu, Yue1; Zhang, Qiang4
关键词Oridonin nanosuspension MCF-7 cells apoptosis
刊名DRUG DELIVERY
2011-05-01
DOI10.3109/10717544.2010.536271
18期:4页:265-271
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
资助者National Basic Research Program of China (973 Program) ; National Nature Science Foundation of China ; National Basic Research Program of China (973 Program) ; National Nature Science Foundation of China
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]SOLID LIPID NANOPARTICLES ; BCL-2 ; DRUGS
英文摘要

The mechanism for anti-tumor activity of oridonin (ORI) nanosuspension, prepared by the high pressure homogenization method, was studied using MCF-7 human breast carcinoma cells in vitro. MTT assay, observation of morphologic changes, flow cytometric analysis, and western blot analysis indicated that ORI nanosuspension could significantly intensify the in vitro anti-tumor activity to MCF-7 cells, as compared with ORI solution. Furthermore, ORI nanosuspension induced G(2)/M stage proliferation arrest and apoptosis in MCF-7 cells depending on its concentration. In addition, western blot analysis indicated that the pro-caspase-3 protein was not cleaved into the activated form and the expression of anti-apoptotic Bcl-2 protein decreased, on the contrary, the expression of pro-apoptotic Bax protein increased in a dose-dependent manner in ORI nanosuspension-treated cells. These observations indicated that the anti-tumor activity of ORI nanosuspension was intensified by cell-cycle arrest and apoptosis induction.

语种英语
所属项目编号2009CB930300 ; 30672674
资助者National Basic Research Program of China (973 Program) ; National Nature Science Foundation of China ; National Basic Research Program of China (973 Program) ; National Nature Science Foundation of China
WOS记录号WOS:000288900100005
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65113
Collection北京大学药学院
作者单位1.Shandong Univ, Dept Pharmaceut, Coll Pharm, Jinan 250012, Shandong, Peoples R China
2.Shandong Univ, Inst Biochem & Mol Biol, Sch Med, Jinan 250012, Shandong, Peoples R China
3.Shandong Univ, Dept Pharmacol, Sch Med, Jinan 250012, Shandong, Peoples R China
4.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Feng, Fei-Fei,Zhang, Dian-Rui,Tian, Ke-Li,et al. Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by oridonin nanosuspension[J]. DRUG DELIVERY,2011,18(4):265-271.
APA Feng, Fei-Fei.,Zhang, Dian-Rui.,Tian, Ke-Li.,Lou, Hai-Yan.,Qi, Xiao-Li.,...&Zhang, Qiang.(2011).Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by oridonin nanosuspension.DRUG DELIVERY,18(4),265-271.
MLA Feng, Fei-Fei,et al."Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by oridonin nanosuspension".DRUG DELIVERY 18.4(2011):265-271.
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