IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Evaluation of hepatic-metastasis risk of colorectal cancer upon the protein signature of PI3K/AKT pathway
Kang, Bin1; Hao, Chunyi2; Wang, Hongyi2; Zhang, Jun1; Xing, Rui1; Shao, Jianmin1; Li, Wenmei2; Xu, Ningzhi1; Lu, Youyong1,2; Liu, Siqi1
关键词colorectal cancer hepatic metastasis PI3K/AKT pathway protein signature
刊名JOURNAL OF PROTEOME RESEARCH
2008-08-01
DOI10.1021/pr800238p
7期:8页:3507-3515
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]NF-KAPPA-B ; LIVER METASTASES ; TNF-ALPHA ; EXPRESSION ; KINASE ; CELLS ; GENE ; PROGRESSION ; CARCINOMAS ; METAPLASIA
英文摘要

Liver is the most common organ of colorectal cancer (CRC) metastasis, and hepatic metastasis (HM) is regulated by complex protein network. Hence, we initiated a proteomic survey to seek interrelated multiplex markers related with HM. A total of 34 unique differential proteins were identified in the primary tumor tissues from 14 CRC patients with/without HM. A differential protein cluster, consisting of 17 proteins throughout PI3K/AKT pathway, was deduced and validated by Western blot. A three-protein signature elicited from the protein cluster, phosphorylated IKB alpha, TNF alpha and MFAP3L, was detected by immunohistochemistry on 105 pairs of CRC and normal samples., The positive protein signature was specifically correlated with HM (P < 0.001), and classified the HM risk of CRC patients with high sensitivity (92.85 +/- 4.87%) and specificity (94.94 +/- 2.5%). The high-risk group had significantly decreased overall survival (P < 0.001). Furthermore, RKO and HT29, two colon cancer cells with different expression status of the protein signature, were used to construct the nude mouse model of HM. And the HM occurrence of RKO cell (4/5) was dramatically higher than that of HT29 cell (1/5). Therefore, the protein signature derived from PI3K/AKT pathway is likely a promising multiplex biomarker for HM of CRC.

语种英语
WOS记录号WOS:000258200400041
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65117
Collection北京大学临床肿瘤学院
北京大学临床肿瘤学院_肝胆胰外二科
北京大学临床肿瘤学院_高技术实验室
作者单位1.Chinese Acad Sci, Beijing Genom Inst, Beijing 101318, Peoples R China
2.Peking Univ, Sch Oncol, Beijing Canc Hosp Inst, Beijing 100036, Peoples R China
Recommended Citation
GB/T 7714
Kang, Bin,Hao, Chunyi,Wang, Hongyi,et al. Evaluation of hepatic-metastasis risk of colorectal cancer upon the protein signature of PI3K/AKT pathway[J]. JOURNAL OF PROTEOME RESEARCH,2008,7(8):3507-3515.
APA Kang, Bin.,Hao, Chunyi.,Wang, Hongyi.,Zhang, Jun.,Xing, Rui.,...&Liu, Siqi.(2008).Evaluation of hepatic-metastasis risk of colorectal cancer upon the protein signature of PI3K/AKT pathway.JOURNAL OF PROTEOME RESEARCH,7(8),3507-3515.
MLA Kang, Bin,et al."Evaluation of hepatic-metastasis risk of colorectal cancer upon the protein signature of PI3K/AKT pathway".JOURNAL OF PROTEOME RESEARCH 7.8(2008):3507-3515.
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