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学科主题: 临床医学
题名:
Evaluation of hepatic-metastasis risk of colorectal cancer upon the protein signature of PI3K/AKT pathway
作者: Kang, Bin1; Hao, Chunyi2; Wang, Hongyi2; Zhang, Jun1; Xing, Rui1; Shao, Jianmin1; Li, Wenmei2; Xu, Ningzhi1; Lu, Youyong1,2; Liu, Siqi1
关键词: colorectal cancer ; hepatic metastasis ; PI3K/AKT pathway ; protein signature
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2008-08-01
DOI: 10.1021/pr800238p
卷: 7, 期:8, 页:3507-3515
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: NF-KAPPA-B ; LIVER METASTASES ; TNF-ALPHA ; EXPRESSION ; KINASE ; CELLS ; GENE ; PROGRESSION ; CARCINOMAS ; METAPLASIA
英文摘要:

Liver is the most common organ of colorectal cancer (CRC) metastasis, and hepatic metastasis (HM) is regulated by complex protein network. Hence, we initiated a proteomic survey to seek interrelated multiplex markers related with HM. A total of 34 unique differential proteins were identified in the primary tumor tissues from 14 CRC patients with/without HM. A differential protein cluster, consisting of 17 proteins throughout PI3K/AKT pathway, was deduced and validated by Western blot. A three-protein signature elicited from the protein cluster, phosphorylated IKB alpha, TNF alpha and MFAP3L, was detected by immunohistochemistry on 105 pairs of CRC and normal samples., The positive protein signature was specifically correlated with HM (P < 0.001), and classified the HM risk of CRC patients with high sensitivity (92.85 +/- 4.87%) and specificity (94.94 +/- 2.5%). The high-risk group had significantly decreased overall survival (P < 0.001). Furthermore, RKO and HT29, two colon cancer cells with different expression status of the protein signature, were used to construct the nude mouse model of HM. And the HM occurrence of RKO cell (4/5) was dramatically higher than that of HT29 cell (1/5). Therefore, the protein signature derived from PI3K/AKT pathway is likely a promising multiplex biomarker for HM of CRC.

语种: 英语
WOS记录号: WOS:000258200400041
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65117
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Chinese Acad Sci, Beijing Genom Inst, Beijing 101318, Peoples R China
2.Peking Univ, Sch Oncol, Beijing Canc Hosp Inst, Beijing 100036, Peoples R China

Recommended Citation:
Kang, Bin,Hao, Chunyi,Wang, Hongyi,et al. Evaluation of hepatic-metastasis risk of colorectal cancer upon the protein signature of PI3K/AKT pathway[J]. JOURNAL OF PROTEOME RESEARCH,2008,7(8):3507-3515.
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