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学科主题临床医学
Hepatoma cells up-regulate expression of programmed cell death-1 in T cells
Chen, Ji1,2; Wu, Xue-Jie1,2; Wang, Gui-Qiang1,2
关键词Hepatoma cell Programmed cell death-1 Protein expression T cell function Cytokine
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2008-11-28
DOI10.3748/wjg.14.6853
14期:44页:6853-6857
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]CHRONIC HEPATITIS-B ; CLINICAL-SIGNIFICANCE ; POTENTIAL MECHANISM ; VIRAL-INFECTION ; PD-1 EXPRESSION ; MOLECULE B7-H1 ; ACTIVATION ; DYSFUNCTION ; RESPONSES ; BLOCKADE
英文摘要

AIM: To investigate the effect of hepatoma cells on up-regulation of programmed cell death-1 (PD-1), and the function of PD-1 on T cells.

METHODS: HepG2 or HepG2.2.1.5 cells were co-cultured with a lymphoma cell line-Jurkat cells. PD-1 expression was detected by flow cytometry. IL-2, INF-gamma and IL-10 in culture supernatant were detected by enzyme-linked immunosorbent assay (ELISA). Cytotoxic action of T cells was determined by MTT reduction assay-direct mononuclear cell cytotoxicity assay.

RESULTS: The PD-1 expression on Jurkat cells increased by 16.17% +/- 2.5% and 17.43% +/- 2.2% after HepG2 or HepG2.2.1.5 cells were co-cultured for 48 h. The levels of IL-2, INF-gamma and IL-10 in the culture supernatant were 202.9 +/- 53.0 pg/mL, 88.6 +/- 4.6 pg/mL and 63.7 +/- 13.4 pg/mL respectively, which were significantly higher than those (102.9 +/- 53 pg/mL, 39.3 +/- 4.2 pg/mL, and 34.6 +/- 13.7 pg/mL) in the control group (P < 0.05). The OD value for MTT assay in the blocking group (0.29 +/- 0.06) was significantly higher than that (0.19 +/- 0.09) in the control group (P < 0.05).

CONCLUSION: PD-1 expression on Jurkat cells is up-regulated by hepatoma cells, cytokines and cytotoxic action are elevated after PD-1/PD-L1 is blocked. (C) 2008 The WJG Press. All rights reserved.

语种英语
所属项目编号30771905
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000261693000013
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65127
专题北京大学第一临床医学院_感染疾病科
作者单位1.Peking Univ, Hosp 1, Dept Infect Dis, Beijing 10034, Peoples R China
2.Peking Univ, Hosp 1, Ctr Liver Dis, Beijing 10034, Peoples R China
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GB/T 7714
Chen, Ji,Wu, Xue-Jie,Wang, Gui-Qiang. Hepatoma cells up-regulate expression of programmed cell death-1 in T cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2008,14(44):6853-6857.
APA Chen, Ji,Wu, Xue-Jie,&Wang, Gui-Qiang.(2008).Hepatoma cells up-regulate expression of programmed cell death-1 in T cells.WORLD JOURNAL OF GASTROENTEROLOGY,14(44),6853-6857.
MLA Chen, Ji,et al."Hepatoma cells up-regulate expression of programmed cell death-1 in T cells".WORLD JOURNAL OF GASTROENTEROLOGY 14.44(2008):6853-6857.
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