IR@PKUHSC  > 北京大学第二临床医学院
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Hepatitis C virus in human B lymphocytes transformed by Epstein-Barr virus in vitro by in situ reverse transcriptase-polymerase chain reaction
Cheng, JL; Liu, BL; Zhang, Y; Bin Tong, W; Yan, Z; Feng, BF
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2001-06-01
7期:3页:370-375
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]BLOOD MONONUCLEAR-CELLS ; HEPATOCELLULAR-CARCINOMA ; ELECTRON-MICROSCOPY ; LIVER-CELLS ; REPLICATION ; RNA ; HYBRIDIZATION ; INFECTION ; LINE ; ESTABLISHMENT
英文摘要

AIM To study persistence and replication of hepatitis C virus (HCV) in patients, peripheral blood monanuclear cells (PBMC) cultured in vitro.

METHODS Epstein-Barr virus (EBV) was used to transform the hepatitis C virus from a HICV positive patient to permanent lymphoblastoid cell lines (LCL). Positive and negative HCV RNA strands of the cultured cells and growth media were detected by reverse transcriptase-polymerase chain reaction ( RT-PCR) each month. Core and NS5 proteins of HCV were further tested using immunohistochemical SP method and in situ RT-PCR.

RESULTS HICV RNA positive strands were consistently detected the cultured cells for one year. The negative-strand RNA in LCL cells and the positive-strand RNA in supernatants were observed intermittently. Immunohistochemical results medicated expression of HCV NS3 and C proteins in LCL cytoplasm mostly. The positive signal of PCR product was dark blue and mainly localized to the LCL cytoplasm. The RT-PCR signal was eliminated by overnight RNase digestion but not DNase digestion.

CONCLUSION HICV may exist and remain functional in a cultured cell line for a long period.

语种英语
WOS记录号WOS:000170371000014
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65133
专题北京大学第二临床医学院
作者单位Beijing Univ, Peoples Hosp, Med Ctr, Inst Hepatol, Beijing 100044, Peoples R China
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GB/T 7714
Cheng, JL,Liu, BL,Zhang, Y,et al. Hepatitis C virus in human B lymphocytes transformed by Epstein-Barr virus in vitro by in situ reverse transcriptase-polymerase chain reaction[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2001,7(3):370-375.
APA Cheng, JL,Liu, BL,Zhang, Y,Bin Tong, W,Yan, Z,&Feng, BF.(2001).Hepatitis C virus in human B lymphocytes transformed by Epstein-Barr virus in vitro by in situ reverse transcriptase-polymerase chain reaction.WORLD JOURNAL OF GASTROENTEROLOGY,7(3),370-375.
MLA Cheng, JL,et al."Hepatitis C virus in human B lymphocytes transformed by Epstein-Barr virus in vitro by in situ reverse transcriptase-polymerase chain reaction".WORLD JOURNAL OF GASTROENTEROLOGY 7.3(2001):370-375.
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