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学科主题: 临床医学
题名:
Hepatitis C virus in human B lymphocytes transformed by Epstein-Barr virus in vitro by in situ reverse transcriptase-polymerase chain reaction
作者: Cheng, JL; Liu, BL; Zhang, Y; Bin Tong, W; Yan, Z; Feng, BF
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2001-06-01
卷: 7, 期:3, 页:370-375
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: BLOOD MONONUCLEAR-CELLS ; HEPATOCELLULAR-CARCINOMA ; ELECTRON-MICROSCOPY ; LIVER-CELLS ; REPLICATION ; RNA ; HYBRIDIZATION ; INFECTION ; LINE ; ESTABLISHMENT
英文摘要:

AIM To study persistence and replication of hepatitis C virus (HCV) in patients, peripheral blood monanuclear cells (PBMC) cultured in vitro.

METHODS Epstein-Barr virus (EBV) was used to transform the hepatitis C virus from a HICV positive patient to permanent lymphoblastoid cell lines (LCL). Positive and negative HCV RNA strands of the cultured cells and growth media were detected by reverse transcriptase-polymerase chain reaction ( RT-PCR) each month. Core and NS5 proteins of HCV were further tested using immunohistochemical SP method and in situ RT-PCR.

RESULTS HICV RNA positive strands were consistently detected the cultured cells for one year. The negative-strand RNA in LCL cells and the positive-strand RNA in supernatants were observed intermittently. Immunohistochemical results medicated expression of HCV NS3 and C proteins in LCL cytoplasm mostly. The positive signal of PCR product was dark blue and mainly localized to the LCL cytoplasm. The RT-PCR signal was eliminated by overnight RNase digestion but not DNase digestion.

CONCLUSION HICV may exist and remain functional in a cultured cell line for a long period.

语种: 英语
WOS记录号: WOS:000170371000014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65133
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: Beijing Univ, Peoples Hosp, Med Ctr, Inst Hepatol, Beijing 100044, Peoples R China

Recommended Citation:
Cheng, JL,Liu, BL,Zhang, Y,et al. Hepatitis C virus in human B lymphocytes transformed by Epstein-Barr virus in vitro by in situ reverse transcriptase-polymerase chain reaction[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2001,7(3):370-375.
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