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学科主题临床医学
Hyperthermia synergizes with tissue factor knockdown to suppress the growth and hepatic metastasis of colorectal cancer in orthotopic tumor model
Li, Hui; Tian, Mao-Lin2; Yu, Ge; Liu, Yu-Cun; Wang, Xin; Zhang, Jing3; Ji, Shi-Qi4; Zhu, Jing; Wan, Yuan-Lian1; Tang, Jian-Qiang
关键词hyperthermia tissue factor colorectal cancer apoptosis matrix metalloproteinase metastasis
刊名JOURNAL OF SURGICAL ONCOLOGY
2012-11-01
DOI10.1002/jso.23136
106期:6页:689-695
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Surgery
研究领域[WOS]Oncology ; Surgery
关键词[WOS]FACTOR EXPRESSION ; GASTRIC-CANCER ; APOPTOSIS ; CELLS ; JNK ; ANGIOGENESIS ; AUTOPHAGY ; PATHWAYS ; CHEMOTHERAPY ; COMBINATION
英文摘要

Background Tissue factor (TF) is a significant risk factor for tumor growth and hepatic metastasis in patients with colorectal cancer (CRC). This study aimed to investigate whether hyperthermia has synergistic anti-tumor effects with TF knockdown in suppressing CRC progression and metastasis in vitro and in vivo. Methods Human colorectal cancer LOVO cells were treated by hyperthermia at 44 degrees C for 2?hr or/and TF siRNA. Then the cells were subjected to colony formation assay. Apoptosis was analyzed by flow cytometry, confocal microscopy, and transmission electron microscopy. The cell migration and invasion abilities were analyzed by wound healing and matrigel assay. In addition, orthotopic nude mice model of CRC was established. Results Hyperthermia synergized with TF knockdown to reduce colony formation ability, induce apoptosis, and suppress the migration and invasion of LOVO cells in vitro. Moreover, hyperthermia in combination with TF depletion inhibited the growth and hepatic metastasis of CRC in orthotopic nude mice model. Mechanistically, the synergistic effects were at least partly mediated by inducing JNK mediated apoptosis and suppressing matrix metalloproteinases (MMPs) mediated invasion. Conclusions Hyperthermia in combination with TF-targeted therapy could be a potential approach for CRC treatment. J. Surg. Oncol. 2012; 106:689695. (c) 2012 Wiley Periodicals, Inc.

语种英语
WOS记录号WOS:000310249900007
项目编号30872469 ; 30801092
资助机构National Natural Sciences Foundation of China ; National Natural Sciences Youth Foundation of China ; "985" Project Foundation of Peking University
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65148
专题北京大学第一临床医学院_普通外科
作者单位1.Peking Univ, Hosp 1, Lab Colon Canc, Dept Gen Surg, Beijing 100034, Peoples R China
2.Capital Med Univ, Beijing Chaoyang Hosp, Dept Hernia & Abdominal Wall Surg, Beijing, Peoples R China
3.Beijing Aerosp Cent Hosp, Dept Cardiovasc Med, Beijing, Peoples R China
4.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
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GB/T 7714
Li, Hui,Tian, Mao-Lin,Yu, Ge,et al. Hyperthermia synergizes with tissue factor knockdown to suppress the growth and hepatic metastasis of colorectal cancer in orthotopic tumor model[J]. JOURNAL OF SURGICAL ONCOLOGY,2012,106(6):689-695.
APA Li, Hui.,Tian, Mao-Lin.,Yu, Ge.,Liu, Yu-Cun.,Wang, Xin.,...&Tang, Jian-Qiang.(2012).Hyperthermia synergizes with tissue factor knockdown to suppress the growth and hepatic metastasis of colorectal cancer in orthotopic tumor model.JOURNAL OF SURGICAL ONCOLOGY,106(6),689-695.
MLA Li, Hui,et al."Hyperthermia synergizes with tissue factor knockdown to suppress the growth and hepatic metastasis of colorectal cancer in orthotopic tumor model".JOURNAL OF SURGICAL ONCOLOGY 106.6(2012):689-695.
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