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学科主题: 临床医学
题名:
Hyperthermia synergizes with tissue factor knockdown to suppress the growth and hepatic metastasis of colorectal cancer in orthotopic tumor model
作者: Li, Hui; Tian, Mao-Lin2; Yu, Ge; Liu, Yu-Cun; Wang, Xin; Zhang, Jing3; Ji, Shi-Qi4; Zhu, Jing; Wan, Yuan-Lian1; Tang, Jian-Qiang
关键词: hyperthermia ; tissue factor ; colorectal cancer ; apoptosis ; matrix metalloproteinase ; metastasis
刊名: JOURNAL OF SURGICAL ONCOLOGY
发表日期: 2012-11-01
DOI: 10.1002/jso.23136
卷: 106, 期:6, 页:689-695
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Surgery
研究领域[WOS]: Oncology ; Surgery
关键词[WOS]: FACTOR EXPRESSION ; GASTRIC-CANCER ; APOPTOSIS ; CELLS ; JNK ; ANGIOGENESIS ; AUTOPHAGY ; PATHWAYS ; CHEMOTHERAPY ; COMBINATION
英文摘要:

Background Tissue factor (TF) is a significant risk factor for tumor growth and hepatic metastasis in patients with colorectal cancer (CRC). This study aimed to investigate whether hyperthermia has synergistic anti-tumor effects with TF knockdown in suppressing CRC progression and metastasis in vitro and in vivo. Methods Human colorectal cancer LOVO cells were treated by hyperthermia at 44 degrees C for 2?hr or/and TF siRNA. Then the cells were subjected to colony formation assay. Apoptosis was analyzed by flow cytometry, confocal microscopy, and transmission electron microscopy. The cell migration and invasion abilities were analyzed by wound healing and matrigel assay. In addition, orthotopic nude mice model of CRC was established. Results Hyperthermia synergized with TF knockdown to reduce colony formation ability, induce apoptosis, and suppress the migration and invasion of LOVO cells in vitro. Moreover, hyperthermia in combination with TF depletion inhibited the growth and hepatic metastasis of CRC in orthotopic nude mice model. Mechanistically, the synergistic effects were at least partly mediated by inducing JNK mediated apoptosis and suppressing matrix metalloproteinases (MMPs) mediated invasion. Conclusions Hyperthermia in combination with TF-targeted therapy could be a potential approach for CRC treatment. J. Surg. Oncol. 2012; 106:689695. (c) 2012 Wiley Periodicals, Inc.

语种: 英语
所属项目编号: 30872469 ; 30801092
项目资助者: National Natural Sciences Foundation of China ; National Natural Sciences Youth Foundation of China ; "985" Project Foundation of Peking University
WOS记录号: WOS:000310249900007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65148
Appears in Collections:北京大学第一临床医学院_普通外科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Lab Colon Canc, Dept Gen Surg, Beijing 100034, Peoples R China
2.Capital Med Univ, Beijing Chaoyang Hosp, Dept Hernia & Abdominal Wall Surg, Beijing, Peoples R China
3.Beijing Aerosp Cent Hosp, Dept Cardiovasc Med, Beijing, Peoples R China
4.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China

Recommended Citation:
Li, Hui,Tian, Mao-Lin,Yu, Ge,et al. Hyperthermia synergizes with tissue factor knockdown to suppress the growth and hepatic metastasis of colorectal cancer in orthotopic tumor model[J]. JOURNAL OF SURGICAL ONCOLOGY,2012,106(6):689-695.
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