IR@PKUHSC  > 北京大学第一临床医学院  > 心血管内科
学科主题临床医学
Apelin protects myocardial injury induced by isoproterenol in rats
Jia, YX; Pan, CS; Zhang, J; Geng, B; Zhao, J; Gerns, H; Yang, J; Chang, JK; Tang, CS; Qi, YF
关键词apelin isoproterenol myocardium APJ mRNA
刊名REGULATORY PEPTIDES
2006-01-15
DOI10.1016/j.regpep.2005.09.033
133期:1-3页:147-154
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Endocrinology & Metabolism ; Physiology
研究领域[WOS]Endocrinology & Metabolism ; Physiology
关键词[WOS]ENDOGENOUS PEPTIDE LIGAND ; ORPHAN RECEPTOR APJ ; BLOOD-PRESSURE ; EXPRESSION ; BINDING ; GENE
英文摘要

We aimed to explore the change in level of apelin and its receptor APJ during myocardial injury and the therapeutic effects of apelin in myocardial injury. Rat myocardial injury was induced by subcutaneous injection of a high dose of isoproterenol (ISO); apelin and APJ mRNA levels were determined by RT-PCR; APJ protein was determined by Western blot; EIA and RIA were used to measure the apelin content and receptor binding, respectively. Plasma lactate dehydrogenase (LDH) activity and myocardial and plasma malondialdehyde (NIDA) contents were higher in ISO-treated hearts than that in controls. ISO-treated rats showed lower +/- LV dp/dt(max) values and higher LVEDP value (all P < 0.01), which suggested severe heart failure. As well, the apelin content in plasma, atrial and ventricular myocardium was decreased by 27%, 30% and 25% (P < 0.01), respectively. The mRNA levels of apelin and APJ in myocardia were also markedly reduced; but the APJ protein level in myocardia was increased. However, administration of apelin significantly ameliorated myocardial injury and ISO-induced heart failure. Compared with the ISO-alone group, the group given low-dosage apelin (5 nmol/kg/day) had 39% and 66% higher +LV dp/dt(max) and -LV dp/dt(max) values, and 40.7% lower LVEDP value (P < 0.01), and the leakage of myocardial LDH and increased MDA content were attenuated (all P < 0.01). Interestingly, bolus injections of apelin (10 nmol/kg/day) resulted in potent inotropic effects in ISO-treated rats. ISO-induced myocardial injury resulted in hypoexpression of apelin and its receptor APJ, and the administration of exogenous apelin ameliorated heart failure and myocardial injury. Apelin could have a cardioprotective effect, and the apelin-APJ system may be a new therapeutic target in myocardial injury and heart failure. (c) 2005 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000234796200021
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被引频次:78[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65164
专题北京大学第一临床医学院_心血管内科
作者单位1.Phoenix Pharmaceut Inc, Belmont, CA 94002 USA
2.Key Lab Minist Educ Mol Cardiol, Beijing 100083, Peoples R China
3.Peking Univ First Hosp, Inst Cardiovasc Res, Beijing 100034, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
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GB/T 7714
Jia, YX,Pan, CS,Zhang, J,et al. Apelin protects myocardial injury induced by isoproterenol in rats[J]. REGULATORY PEPTIDES,2006,133(1-3):147-154.
APA Jia, YX.,Pan, CS.,Zhang, J.,Geng, B.,Zhao, J.,...&Qi, YF.(2006).Apelin protects myocardial injury induced by isoproterenol in rats.REGULATORY PEPTIDES,133(1-3),147-154.
MLA Jia, YX,et al."Apelin protects myocardial injury induced by isoproterenol in rats".REGULATORY PEPTIDES 133.1-3(2006):147-154.
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