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学科主题: 临床医学
题名:
Apelin protects myocardial injury induced by isoproterenol in rats
作者: Jia, YX; Pan, CS; Zhang, J; Geng, B; Zhao, J; Gerns, H; Yang, J; Chang, JK; Tang, CS; Qi, YF
关键词: apelin ; isoproterenol ; myocardium ; APJ ; mRNA
刊名: REGULATORY PEPTIDES
发表日期: 2006-01-15
DOI: 10.1016/j.regpep.2005.09.033
卷: 133, 期:1-3, 页:147-154
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Endocrinology & Metabolism ; Physiology
研究领域[WOS]: Endocrinology & Metabolism ; Physiology
关键词[WOS]: ENDOGENOUS PEPTIDE LIGAND ; ORPHAN RECEPTOR APJ ; BLOOD-PRESSURE ; EXPRESSION ; BINDING ; GENE
英文摘要:

We aimed to explore the change in level of apelin and its receptor APJ during myocardial injury and the therapeutic effects of apelin in myocardial injury. Rat myocardial injury was induced by subcutaneous injection of a high dose of isoproterenol (ISO); apelin and APJ mRNA levels were determined by RT-PCR; APJ protein was determined by Western blot; EIA and RIA were used to measure the apelin content and receptor binding, respectively. Plasma lactate dehydrogenase (LDH) activity and myocardial and plasma malondialdehyde (NIDA) contents were higher in ISO-treated hearts than that in controls. ISO-treated rats showed lower +/- LV dp/dt(max) values and higher LVEDP value (all P < 0.01), which suggested severe heart failure. As well, the apelin content in plasma, atrial and ventricular myocardium was decreased by 27%, 30% and 25% (P < 0.01), respectively. The mRNA levels of apelin and APJ in myocardia were also markedly reduced; but the APJ protein level in myocardia was increased. However, administration of apelin significantly ameliorated myocardial injury and ISO-induced heart failure. Compared with the ISO-alone group, the group given low-dosage apelin (5 nmol/kg/day) had 39% and 66% higher +LV dp/dt(max) and -LV dp/dt(max) values, and 40.7% lower LVEDP value (P < 0.01), and the leakage of myocardial LDH and increased MDA content were attenuated (all P < 0.01). Interestingly, bolus injections of apelin (10 nmol/kg/day) resulted in potent inotropic effects in ISO-treated rats. ISO-induced myocardial injury resulted in hypoexpression of apelin and its receptor APJ, and the administration of exogenous apelin ameliorated heart failure and myocardial injury. Apelin could have a cardioprotective effect, and the apelin-APJ system may be a new therapeutic target in myocardial injury and heart failure. (c) 2005 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000234796200021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65164
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.Phoenix Pharmaceut Inc, Belmont, CA 94002 USA
2.Key Lab Minist Educ Mol Cardiol, Beijing 100083, Peoples R China
3.Peking Univ First Hosp, Inst Cardiovasc Res, Beijing 100034, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China

Recommended Citation:
Jia, YX,Pan, CS,Zhang, J,et al. Apelin protects myocardial injury induced by isoproterenol in rats[J]. REGULATORY PEPTIDES,2006,133(1-3):147-154.
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