IR@PKUHSC  > 北京大学药学院  > 化学生物学系
学科主题药学
La3+ stimulate the activity of calcineurin in two different ways
Hu, J; Yang, X; Wang, K
关键词calcineurin calmodulin lanthanum ion
刊名JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
2005-10-01
DOI10.1007/s00775-005-0021-5
10期:6页:704-711
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Inorganic & Nuclear
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]METAL-IONS ; BIOTINYLATED CALMODULIN ; PHOSPHATASE ; ACTIVATION ; BINDING ; DOMAIN ; INHIBITION ; NEURONS ; COMPLEX ; TARGET
英文摘要

It is well known that the activity of calcineurin (CaN) could be modulated by several transitional metal ions. In the present work, the effects of a calcium analog, lanthanum ion (La3+), on the activity of CaN were studied. It was found that La3+ exerted multiple effects on CaN activity. La3+ could stimulate CaN in the absence of calmodulin (CaM); whereas at low concentrations of La3+, there was a slight inhibition of activation of CaN in the presence of CaM. Competitive experiments and limited trypsin proteolysis confirmed that La3+ did not act on the catalytic core of CaN, but exerted its effect through direct action on the CaN regulatory domain similar to Mg2+. In activity titration and spot blotting studies, La3+-containing CaM complexes were less effective in stimulating CaN than Ca2+ or Mn2+-containing CaM; however, the binding affinity of these metal-CaM complexes to CaN was similar. These effects of La3+ on CaN activity are unique among metal ions and may provide clues to understand the biological effects of La3+.

语种英语
WOS记录号WOS:000233135700012
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65190
Collection北京大学药学院_化学生物学系
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Natl Res Labs Nat & Biomimet Drugs, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Hu, J,Yang, X,Wang, K. La3+ stimulate the activity of calcineurin in two different ways[J]. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY,2005,10(6):704-711.
APA Hu, J,Yang, X,&Wang, K.(2005).La3+ stimulate the activity of calcineurin in two different ways.JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY,10(6),704-711.
MLA Hu, J,et al."La3+ stimulate the activity of calcineurin in two different ways".JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY 10.6(2005):704-711.
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