|PD-1 expression on peripheral CD8+TEM/TEMRA subsets closely correlated with HCV viral load in chronic hepatitis C patients|
|Shen, Tao1; Zheng, Jiajia1; Xu, Chunhui1; Liu, Jia1; Zhang, Weidong2; Lu, Fengmin1; Zhuang, Hui1|
|WOS标题词||Science & Technology|
|关键词[WOS]||CD8(+) T-CELLS ; IMMUNODEFICIENCY-VIRUS TYPE-1 ; COMMERCIAL PLASMA DONORS ; HIV-INFECTION ; MEMORY CELLS ; CD127 EXPRESSION ; EFFECTOR ; LYMPHOCYTES ; INTERLEUKIN-7 ; RESPONSES|
Background: Tight correlation between host circulating CD8+ T cell-mediated immune response and control of viral replication is classical characteristic of long-term HCV infection. CD8+ T cell maturation/activation markers are expected to be associated with viral replication and disease progression in chronic HCV infection. The aim of the present study was to explore novel markers on CD8+ T cells with ability to evaluate HCV viral replication and disease progression.
Methods: PBMCs were isolated from 37 chronic HCV-infected patients and 17 healthy controls. Distributed pattern of CD8+ T cells subsets and expression of PD-1, CD38, HLA-DR and CD127 were analyzed by flow cytometry. The correlation between expression of surface markers and HCV viral load or ALT was studied.
Results: Declined naive and increased TEMRA CD8+ T subsets were found in HCV-infected individuals compared with healthy controls. Percentage and MFI of PD-1, CD38 and HLA-DR on all CD8+ T cell subsets were higher in HCV-infected patients than healthy controls. In contrast, CD127 expression on CD8+ TCM showed an opposite trend as PD-1, CD38 and HLA-DR did. In chronic HCV infection, MFI of PD-1 on CD8+ TEM (p < 0.0001) and TEMRA (p = 0.0015) was positively correlated with HCV viral load while HLA-DR expression on non-naive CD8+ T cell subsets (p < 0.05) was negatively correlated with HCV viral load.
Conclusion: PD-1 level on peripheral CD8+ TEM/TEMRA was highly correlated with HCV viral load in chronic HCV-infected patients, which made PD-1 a novel indicator to evaluate HCV replication and disease progression in chronic hepatitis C patients.
|资助机构||China National ST Major Project|
|作者单位||1.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100191, Peoples R China|
2.Zhengzhou Univ, Dept Epidemiol, Coll Publ Hlth, Zhengzhou 450052, Henan, Peoples R China
|Shen, Tao,Zheng, Jiajia,Xu, Chunhui,et al. PD-1 expression on peripheral CD8+TEM/TEMRA subsets closely correlated with HCV viral load in chronic hepatitis C patients[J]. VIROLOGY JOURNAL,2010,7(1).|
|APA||Shen, Tao.,Zheng, Jiajia.,Xu, Chunhui.,Liu, Jia.,Zhang, Weidong.,...&Zhuang, Hui.(2010).PD-1 expression on peripheral CD8+TEM/TEMRA subsets closely correlated with HCV viral load in chronic hepatitis C patients.VIROLOGY JOURNAL,7(1).|
|MLA||Shen, Tao,et al."PD-1 expression on peripheral CD8+TEM/TEMRA subsets closely correlated with HCV viral load in chronic hepatitis C patients".VIROLOGY JOURNAL 7.1(2010).|
|PD-1 expression on p（1908KB）||期刊论文||出版稿||开放获取||CC BY-NC-SA||浏览 请求全文|