|Anti-Helicobacter pylori activities of Chenopodium ambrosioides L. in vitro and in vivo|
|Ye, Hui1; Liu, Yu2; Li, Ning1; Yu, Jing1; Cheng, Hong3; Li, Jiang3; Zhang, Xue-Zhi1|
|关键词||Helicobacter pylori Bactericidal activity Chenopodium ambrosioides L. Phytotherapy|
|刊名||WORLD JOURNAL OF GASTROENTEROLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||PROTON PUMP INHIBITOR ; TRIPLE THERAPY ; CONSENSUS REPORT ; ERADICATION ; RESISTANCE ; INFECTION ; ANTIBIOTICS ; MANAGEMENT ; TRIAL ; CHINA|
AIM: To investigate the bactericidal effects of Chenopo-dium ambrosioides L. (CAL) against Helicobacter pylori (H. pylori) both in vitro and in vivo.
METHODS: For in vitro experiments, the inhibitory activity of CAL was tested using an agar dilution method; H. pylori strain NCTC11637 was incubated on Columbia blood agar plates containing serial concentrations of CAL. The minimal inhibitory concentration (MIC) was determined by the absence of H. pylori colonies on the agar plate. Time-kill curves were used to evaluate bactericidal activity; the average number of colonies was calculated at 0, 2, 8 and 24 h after liquid incubation with concentrations of CAL at 0.5, 1, and 2 x MIC. For in vivo experiments, H. pylori -infected mice were randomly divided into CAL, triple therapy (lansoprazole, metronidazole, and clarithromycin), blank control, or H. pylori control groups. The eradication ratios were determined by positive findings from rapid urease tests (RUTs) and by histopathology.
RESULTS: In vitro, the MIC of CAL against H. pylori was 16 mg/L. The time-kill curves showed a stable and persistent decreasing tendency with increasing CAL concentration, and the intensity of the bactericidal effect was proportional to dose; the 1 and 2 x MIC completely inhibited the growth of H. pylori at 24 h. In vivo, the eradication ratios in the CAL group were 60% (6/10) by RUT and 50% (5/10) by histopathology. Ratios in the triple therapy group were both 70% (7/10), and there was no difference between the CAL and triple therapy groups. Histopathologic evaluation revealed massive bacterial colonization on the surface of gastric mucosa and slight infiltration of mononuclear cells after inoculation with H. pylori, but no obvious inflammation or other pathologic changes in gastric mucosa of mice from CAL and triple therapy groups.
CONCLUSION: CAL demonstrates effective bactericidal activity against H. pylori both in vitro and in vivo.
|资助机构||National Natural Science Foundation Project of China|
|作者单位||1.Peking Univ, Dept Integrated Tradit Chinese Med, Hosp 1, Beijing 100034, Peoples R China|
2.Peking Univ, Int Hosp, Dept Tradit Chinese Med, Beijing 102206, Peoples R China
3.Peking Univ, Dept Gastroenterol, Hosp 1, Beijing 100034, Peoples R China
|Ye, Hui,Liu, Yu,Li, Ning,et al. Anti-Helicobacter pylori activities of Chenopodium ambrosioides L. in vitro and in vivo[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2015,21(14):4178-4183.|
|APA||Ye, Hui.,Liu, Yu.,Li, Ning.,Yu, Jing.,Cheng, Hong.,...&Zhang, Xue-Zhi.(2015).Anti-Helicobacter pylori activities of Chenopodium ambrosioides L. in vitro and in vivo.WORLD JOURNAL OF GASTROENTEROLOGY,21(14),4178-4183.|
|MLA||Ye, Hui,et al."Anti-Helicobacter pylori activities of Chenopodium ambrosioides L. in vitro and in vivo".WORLD JOURNAL OF GASTROENTEROLOGY 21.14(2015):4178-4183.|