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学科主题: 临床医学
题名:
A simple and sensitive LC-MS/MS method for the determination of sotalol in rat plasma
作者: Feng, Zhiping1,2; Yu, Siyuan1,2; Liu, Wei1; Yang, Li1; Liu, Yang1; Zhai, Suodi1; Wang, Fang2; Zhang, Xianhua1
关键词: sotalol ; LC-MS ; MS ; rat plasma ; validation
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2015-08-01
DOI: 10.1002/bmc.3403
卷: 29, 期:8, 页:1167-1172
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: TANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; SOLID-PHASE EXTRACTION ; BETA-BLOCKERS ; CLASSIFICATION
英文摘要:

A sensitive, rapid and robust HPLC method with tandem mass spectrometry (HPLC/MS/MS) detection has been developed and validated for the quantification of sotalol in rat plasma. Plasma samples were precipitated with acetonitrile before analysis. The chromatographic separation was performed on an Atlantis hydrophilic interaction liquid chromatography Silica column (50x2.1mm, 3 mu m) with a gradient mobile phase of 10mm NH4COOH (containing 0.2% of formic acid) as buffer A and acetonitrile as mobile phase B. Sotalol (m/z 273.2255.1) and atenolol (the internal standard, IS, m/z 267.2190.1) were monitored under positive ionization mode with 5500 QTRAP. Retention time of sotalol and the IS were 2.69 and 3.43min, respectively. The linear range was 5-500nm based on the analysis of 0.1mL of plasma. The intrabatch precision ranged from 1.2 to 6.1%, and the inter-batch precision was from 3.3 to 6.5%. The coefficient of variation of IS-normalized matrix factor was 7.6%. Experiments for stability were performed and the analyte was sufficiently stable. A run time of 6min for each injection made it possible to analyze a high throughput of plasma samples. The assay was successfully applied to the determination of sotalol in rat plasma after a micro-dose oral administration. Copyright (c) 2014 John Wiley & Sons, Ltd.

语种: 英语
WOS记录号: WOS:000357994000004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65241
Appears in Collections:北京大学第三临床医学院_药剂科_期刊论文

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作者单位: 1.Peking Univ, Dept Pharm, Hosp 3, Beijing 100191, Peoples R China
2.Affiliated Jiangmen Hosp Sun Yat Sen, Jiangmen Cent Hosp, Dept Pharm, Jiangmen City 529000, Guangdong, Peoples R China

Recommended Citation:
Feng, Zhiping,Yu, Siyuan,Liu, Wei,et al. A simple and sensitive LC-MS/MS method for the determination of sotalol in rat plasma[J]. BIOMEDICAL CHROMATOGRAPHY,2015,29(8):1167-1172.
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