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学科主题: 临床医学
题名:
Study on the malignant and normal rectum tissues using NMR spectroscopy
作者: Gao Xiu-xiang1; He Wen-yi2; Yao Hong-wei3; Du Jun-kai4; Zhao Mei-xian1,5; Qi Jian1; Li Hui-zhen1,6; Xu Yi-zhuang1; Wu Jin-guang1
关键词: high resolution magic angle spinning ; nuclear magnetic resonance ; rectum cancer ; early diagnosis
刊名: SPECTROSCOPY AND SPECTRAL ANALYSIS
发表日期: 2008-09-01
DOI: 10.3964/j.issn.1000-0593(2008)09-2201-06
卷: 28, 期:9, 页:2201-2206
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Spectroscopy
研究领域[WOS]: Spectroscopy
关键词[WOS]: BREAST-CANCER TISSUE ; EX-VIVO ; IN-VIVO ; INFRARED-SPECTROSCOPY ; MRS ; DIAGNOSIS ; TUMOR ; H-1
英文摘要:

In the present paper, NMR spectroscopy, an effective tool to detect the variation in molecular structure and changes in chemical composition of metabolites in tissues, was used to study the differences between malignant and normal tissues from rectum. H-1 spectra of four malignant rectum tissue samples and two normal control tissues were investigated by using a 500M NMR high-resolution magic angle spinning magnetic resonance spectrometers (HR-MAS NMR). The results indicate that the H-1 HRMAS spectra of rectum cancer tissues are significantly different from those of the normal controls and most differences are pres ents in the form of variation in the relative intensities of the characteristic peak of various metabolites. In order to characterize the variation in the relative intensities in a quantitative manner, the intensity of the methyl peak of fatty acid at 0.88 was utilized as inner standard. Systematic differences between NMR spectra of malignant tissue and normal controls are as follows: (1) The concentration of amino acid increases significantly in malignant tissues, since the relative intensities of characteristic peaks of amino acid including valine, isoleucine, leucine, lysine, glutamate, glutamine, and aspartate are stronger in the NMR spectra of the malignant tissues. This phenomenon may reflect the fact that the activity of protein synthesis is enhanced in cancerous tissues. (2) The intensities of the characteristic peaks of lactic acid in malignant tissues are higher than those from normal controls. This may be related to the nature of anaerobic metabolism activity in malignant tissues. (3) The level of choline and its derivatives, taurine and creatine, increases significantly in malignant tissues, suggesting that the metabolic activity of malignant tissues changes. (4) In the spectral region between 4.5 and 10, observable changes occur on the peaks for unsaturated fatty acid and nuclear acids. Therefore, the above spectral variations in high resolution magic angle spinning NMR spectroscopy may be utilized as a potential tool to diagnose rectum cancer.

语种: 中文
WOS记录号: WOS:000259688600057
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65244
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Chinese Acad Med Sci, Peking Union Med Coll, Inst Mat Med, Beijing 100050, Peoples R China
3.Peking Univ, Hosp 3, Beijing 100083, Peoples R China
4.Xi An Jiao Tong Univ, Hosp 1, Xian 710061, Peoples R China
5.Beijing Univ Chem Technol, Sch Sci, Dept Appl Chem, Beijing 100029, Peoples R China
6.Henan Normal Univ, Coll Chem & Environm Sci, Xinxiang 453007, Peoples R China

Recommended Citation:
Gao Xiu-xiang,He Wen-yi,Yao Hong-wei,et al. Study on the malignant and normal rectum tissues using NMR spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2008,28(9):2201-2206.
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