|Adenovirus-mediated GDNF protects cultured motoneurons from glutamate injury|
|Tang, XQ; Wang, Y; Han, JS; Wan, Y
excitatory amino acid
glia cell line-derived neurotrophic factor
|WOS标题词||Science & Technology
|研究领域[WOS]||Neurosciences & Neurology
The protective effects of adenovirus-mediated glia cell line-derived neurotrophic factor (GDNF) gene transaction was investigated on cultured motoneurons. First, the dose- and time-response relationship of glutamate neurotoxicity was determined on spinal motoneuron cultures. Then, the effect of the gdnf recombinant adenovirus (AdCMVgdnf) was tested in this cellular model. AdCMVgdnf at 20 MOI (multiplicity of infection) was found to significantly reduce the cell loss of motoneurons, as compared to AdCMV gdnf at 20 MOI, the recombinant adenovirus containing the marker gene lacZ Furthermore, the adenovirus was proved to mediate erogenous gene expression using X-Gal staining and a semi-quantitative RT-PCR method. These results suggested a therapeutic potential of adenovirus vector-mediated gdnf gene therapy in human motoneuron diseases. NeuroReport 12:3073-3076 (C) 2001 Lippincott Williams & Wilkins.
|作者单位||Beijing Univ, Neurosci Res Inst, Beijing 100083, Peoples R China|
Tang, XQ,Wang, Y,Han, JS,et al. Adenovirus-mediated GDNF protects cultured motoneurons from glutamate injury[J]. NEUROREPORT,2001,12(14):3073-3076.
Tang, XQ,Wang, Y,Han, JS,&Wan, Y.(2001).Adenovirus-mediated GDNF protects cultured motoneurons from glutamate injury.NEUROREPORT,12(14),3073-3076.
Tang, XQ,et al."Adenovirus-mediated GDNF protects cultured motoneurons from glutamate injury".NEUROREPORT 12.14(2001):3073-3076.
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