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学科主题: 基础医学
题名:
Adenovirus-mediated GDNF protects cultured motoneurons from glutamate injury
作者: Tang, XQ; Wang, Y; Han, JS; Wan, Y
关键词: adenovirus ; excitatory amino acid ; gene therapy ; glia cell line-derived neurotrophic factor ; glutamate ; motoneuron ; spinal cord
刊名: NEUROREPORT
发表日期: 2001-10-08
卷: 12, 期:14, 页:3073-3076
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: AMYOTROPHIC-LATERAL-SCLEROSIS ; SPINAL-CORD ; GENE-EXPRESSION ; MOTOR-NEURONS ; CELL-CULTURE ; IN-VITRO ; NEUROTOXICITY ; BRAIN ; RECEPTORS ; ASPARTATE
英文摘要:

The protective effects of adenovirus-mediated glia cell line-derived neurotrophic factor (GDNF) gene transaction was investigated on cultured motoneurons. First, the dose- and time-response relationship of glutamate neurotoxicity was determined on spinal motoneuron cultures. Then, the effect of the gdnf recombinant adenovirus (AdCMVgdnf) was tested in this cellular model. AdCMVgdnf at 20 MOI (multiplicity of infection) was found to significantly reduce the cell loss of motoneurons, as compared to AdCMV gdnf at 20 MOI, the recombinant adenovirus containing the marker gene lacZ Furthermore, the adenovirus was proved to mediate erogenous gene expression using X-Gal staining and a semi-quantitative RT-PCR method. These results suggested a therapeutic potential of adenovirus vector-mediated gdnf gene therapy in human motoneuron diseases. NeuroReport 12:3073-3076 (C) 2001 Lippincott Williams & Wilkins.

语种: 英语
WOS记录号: WOS:000171232800017
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65245
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: Beijing Univ, Neurosci Res Inst, Beijing 100083, Peoples R China

Recommended Citation:
Tang, XQ,Wang, Y,Han, JS,et al. Adenovirus-mediated GDNF protects cultured motoneurons from glutamate injury[J]. NEUROREPORT,2001,12(14):3073-3076.
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