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学科主题基础医学
Adenovirus-mediated GDNF protects cultured motoneurons from glutamate injury
Tang, XQ; Wang, Y; Han, JS; Wan, Y
关键词adenovirus excitatory amino acid gene therapy glia cell line-derived neurotrophic factor glutamate motoneuron spinal cord
刊名NEUROREPORT
2001-10-08
12期:14页:3073-3076
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]AMYOTROPHIC-LATERAL-SCLEROSIS ; SPINAL-CORD ; GENE-EXPRESSION ; MOTOR-NEURONS ; CELL-CULTURE ; IN-VITRO ; NEUROTOXICITY ; BRAIN ; RECEPTORS ; ASPARTATE
英文摘要

The protective effects of adenovirus-mediated glia cell line-derived neurotrophic factor (GDNF) gene transaction was investigated on cultured motoneurons. First, the dose- and time-response relationship of glutamate neurotoxicity was determined on spinal motoneuron cultures. Then, the effect of the gdnf recombinant adenovirus (AdCMVgdnf) was tested in this cellular model. AdCMVgdnf at 20 MOI (multiplicity of infection) was found to significantly reduce the cell loss of motoneurons, as compared to AdCMV gdnf at 20 MOI, the recombinant adenovirus containing the marker gene lacZ Furthermore, the adenovirus was proved to mediate erogenous gene expression using X-Gal staining and a semi-quantitative RT-PCR method. These results suggested a therapeutic potential of adenovirus vector-mediated gdnf gene therapy in human motoneuron diseases. NeuroReport 12:3073-3076 (C) 2001 Lippincott Williams & Wilkins.

语种英语
WOS记录号WOS:000171232800017
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65245
专题北京大学基础医学院_神经生物学系
作者单位Beijing Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
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GB/T 7714
Tang, XQ,Wang, Y,Han, JS,et al. Adenovirus-mediated GDNF protects cultured motoneurons from glutamate injury[J]. NEUROREPORT,2001,12(14):3073-3076.
APA Tang, XQ,Wang, Y,Han, JS,&Wan, Y.(2001).Adenovirus-mediated GDNF protects cultured motoneurons from glutamate injury.NEUROREPORT,12(14),3073-3076.
MLA Tang, XQ,et al."Adenovirus-mediated GDNF protects cultured motoneurons from glutamate injury".NEUROREPORT 12.14(2001):3073-3076.
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