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学科主题临床医学
Interleukin-21 Induces Proliferation and Proinflammatory Cytokine Profile of Fibroblast-like Synoviocytes of Patients with Rheumatoid Arthritis
Xing, R.1; Yang, L.1; Jin, Y.1; Sun, L.1; Li, C.1; Li, Z.2; Zhao, J.1; Liu, X.1
刊名SCANDINAVIAN JOURNAL OF IMMUNOLOGY
2016
DOI10.1111/sji.12396
83期:1页:64-71
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology
研究领域[WOS]Immunology
关键词[WOS]SYNOVIAL FIBROBLASTS ; CELL EXPANSION ; TH17 CELLS ; T-CELLS ; RECEPTOR ; IL-21 ; DISEASE ; AUTOIMMUNITY ; INHIBITION ; ACTIVATION
英文摘要

Fibroblast-like synoviocytes (FLS) play a pivotal role in the pathogenesis of rheumatoid arthritis (RA) through aggressive proliferation and invasion, and certain proinflammatory cytokines may affect synoviocyte proliferation. To evaluate whether interleukin-21 (IL-21) could promote proliferation and proinflammatory cytokine production by RA-FLS, immunohistochemistry and immunoblotting were performed to observe the expression of IL-21 receptor (IL-21R) in synovial tissues and FLS from RA and osteoarthritis (OA) patients. The MTS assay was used to analyse RA-FLS proliferation. The concentrations of IL-6 and tumour necrosis factor-alpha (TNF-alpha) in culture supernatants were determined by enzyme-linked immunosorbent assay (ELISA). The signalling pathways triggered by IL-21 were characterized by immunoblotting. IL-21R was upregulated in the synovial tissues and FLS of RA patients as compared with OA patients. IL-21 stimulated RA-FLS proliferation and promoted the production of TNF- and IL-6 and blockade of IL-21/IL-21R pathway with IL-21R.Fc attenuated IL-21-induced proliferation and secretion of TNF-alpha and IL-6. Moreover, IL-21 induced activation of the ERK1/2, PI3K/AKT and STAT3 pathways, and blockade of these pathways attenuated IL-21-induced proliferation and secretion of TNF-alpha and IL-6. These results suggest that IL-21 could promote RA-FLS proliferation and production of proinflammatory cytokines. Therefore, therapeutic strategies targeting IL-21 might be effective for the treatment of RA.

语种英语
WOS记录号WOS:000366927600010
项目编号81102255 ; 81273293 ; 81471599
资助机构National Natural Science Foundation of China
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65253
专题北京大学第三临床医学院
作者单位1.Peking Univ, Hosp 3, Dept Rheumatol & Immunol, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Anesthesiol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Xing, R.,Yang, L.,Jin, Y.,et al. Interleukin-21 Induces Proliferation and Proinflammatory Cytokine Profile of Fibroblast-like Synoviocytes of Patients with Rheumatoid Arthritis[J]. SCANDINAVIAN JOURNAL OF IMMUNOLOGY,2016,83(1):64-71.
APA Xing, R..,Yang, L..,Jin, Y..,Sun, L..,Li, C..,...&Liu, X..(2016).Interleukin-21 Induces Proliferation and Proinflammatory Cytokine Profile of Fibroblast-like Synoviocytes of Patients with Rheumatoid Arthritis.SCANDINAVIAN JOURNAL OF IMMUNOLOGY,83(1),64-71.
MLA Xing, R.,et al."Interleukin-21 Induces Proliferation and Proinflammatory Cytokine Profile of Fibroblast-like Synoviocytes of Patients with Rheumatoid Arthritis".SCANDINAVIAN JOURNAL OF IMMUNOLOGY 83.1(2016):64-71.
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