IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
High Detection Rates of Colorectal Neoplasia by Stool DNA Testing With a Novel Digital Melt Curve Assay
Zou, Hongzhi1; Taylor, William R.1; Harrington, Jonathan J.1; Hussain, Fareeda Taher Nazer1; Cao, Xiaoming1; Loprinzi, Charles L.2; Levine, Theodore R.4; Rex, Douglas K.5; Ahnen, Dennis6,7; Knigge, Kandice L.8; Lance, Peter9; Jiang, Xuan10; Smith, David I.3; Ahlquist, David A.1
刊名GASTROENTEROLOGY
2009-02-01
DOI10.1053/j.gastro.2008.10.023
136期:2页:459-470
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]FECAL-OCCULT-BLOOD ; K-RAS MUTATIONS ; CANCER PATIENTS ; LUNG-CANCER ; GEFITINIB ; PLASMA ; PCR ; QUANTIFICATION ; RESPONSIVENESS ; SURVEILLANCE
英文摘要

Background & Aims: Current stool DNA tests identify about half of individuals with colorectal cancers and miss most individuals with advanced adenomas. We developed a digital melt curve (DMC) assay to quantify low-abundance mutations in stool samples for detection of colorectal. neoplasms and compared this test with other approaches. Methods: We combined a melt curve assay with digital polymerase chain reaction and validated the quantitative range. We then evaluated its ability to detect neoplasms in 2 clinical studies. In study 1, stool samples from patients with colorectal tumors with known mutations (KRAS, APC, BRAF, TP53) were assayed. In study II, archived stool samples from patients with advanced adenomas containing known KRAS mutations were assayed, along with controls. Results were compared with those from the stool DNA test PreGenPlus (Exact Sciences, Marlborough, MA), Hemoccult, and HemoccultSensa (both Beckman-Coulter, Fullerton, CA). Results : The DMC assay detected samples in which only 0.1% of target genes were mutated. In study 1, the DMC assay detected known mutations in 28 (90%) of 31 tumor samples and 6 (75%) of 8 advanced adenoma samples. In study 11, the DMC assay detected 16 (59%) of 27 advanced adenoma samples that contained KRAS mutations, compared with 7% with the Hemoccult, 15% with the HemoccultSensa, and 26% with the PreGenPlus assays (P < .05 for each, compared with the DMC assay); specificities did not differ significantly. Conclusions: The DMC assay has a high level of sensitivity in detecting individuals with colon neoplasms and is better than current stool screening methods in detecting those with advanced adenomas. Further studies are indicated.

语种英语
WOS记录号WOS:000262967700017
资助机构Charles Oswald ; Eddie and Dana Gong
引用统计
被引频次:73[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65260
专题北京大学第二临床医学院
北京大学第二临床医学院_消化科
作者单位1.Kaiser Permanente Med Care Program, Oakland, CA USA
2.Mayo Clin, Div Med Oncol, Rochester, MN 55905 USA
3.Mayo Clin, Div Expt Pathol, Rochester, MN 55905 USA
4.Mayo Clin, Miles & Shirley Fiterman Div Gastroenterol & Hepa, Rochester, MN 55905 USA
5.Indiana Univ, Sch Med, Div Gastroenterol, Indianapolis, IN USA
6.Univ Colorado, Hlth Sci Ctr, Denver, CO USA
7.Dept Vet Affairs Med Ctr, Denver, CO USA
8.Oregon Hlth & Sci Univ, Div Gastroenterol, Portland, OR 97201 USA
9.Univ Arizona, Arizona Canc Ctr, Tucson, AZ USA
10.Peking Univ, Peoples Hosp, Div Gastroenterol & Hepatol, Beijing 100871, Peoples R China
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GB/T 7714
Zou, Hongzhi,Taylor, William R.,Harrington, Jonathan J.,et al. High Detection Rates of Colorectal Neoplasia by Stool DNA Testing With a Novel Digital Melt Curve Assay[J]. GASTROENTEROLOGY,2009,136(2):459-470.
APA Zou, Hongzhi.,Taylor, William R..,Harrington, Jonathan J..,Hussain, Fareeda Taher Nazer.,Cao, Xiaoming.,...&Ahlquist, David A..(2009).High Detection Rates of Colorectal Neoplasia by Stool DNA Testing With a Novel Digital Melt Curve Assay.GASTROENTEROLOGY,136(2),459-470.
MLA Zou, Hongzhi,et al."High Detection Rates of Colorectal Neoplasia by Stool DNA Testing With a Novel Digital Melt Curve Assay".GASTROENTEROLOGY 136.2(2009):459-470.
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