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IgG subclass distribution, affinity of anti-myeloperoxidase antibodies in sera from patients with Wegener′s granulomatosis and microscopic polyangiitis
Liu, Li-Jun; Chen, Min; Yu, Feng; Zhao, Ming-Hui; Wang, Hai-Yan
关键词affinity anti-myeloperoxidase antibodies epitope microscopic polyangiitis subclass Wegener&prime s granulomatosis
刊名NEPHROLOGY
2008-10-01
DOI10.1111/j.1440-1797.2008.00976.x
13期:7页:629-635
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]CYTOPLASMIC AUTOANTIBODIES ANCA ; SYSTEMIC VASCULITIS ; MPO-ANCA ; CHINESE PATIENTS ; MYELOPEROXIDASE ; EPITOPES ; PROTEINASE-3
英文摘要

Aim: Our previous study suggested that patients with M PO-AN CA (myeloperoxidase antineutrophil cytoplasmic autoantibodies)-positive Wegener′s granulomatosis (WG) were common in Chinese, indicating that patients with MPO-ANCA could manifest as either WG or microscopic polyangiitis (MPA). The aim of this study was to compare the immunological characteristics of MPO-ANCA in patients with WG and MPA.

Methods: Fifteen patients with WG and 21 patients with MPA were enrolled in the current study. Anti-MPO IgG subclasses and their titres were detected by antigen-specific enzyme-linked immunosorbent assays (ELISA); affinity was assessed by antigen-inhibition ELISAs. The sera from all patients were employed to inhibit biotin conjugated affinity-purified human anti-MPO antibodies (Probe-biotin), from plasma exchange of a patient with MPA, in a competitive inhibition ELISAs system.

Results: All four anti-MPO subclasses could be detected in sera from patients with WG and MPA. The titres of anti-MPO IgG4 subclass in patients with WG were significantly higher than those with MPA (1:1878 vs 1:218, P< 0.005).The affinity constants of MPO-ANCA were comparable between patients with WG and MPA(0.3-70/M vs 0.3-140/M respectively). Eleven out of the 15 sera and 18 out of the 21 sera could inhibit the binding of the Probe-biotin in patients with WG and MPA respectively. The average inhibition rate was 47.7% +/- 11.50% and 61.7% +/- 14.5% respectively (P < 0.05).

Conclusion: MPO-ANCA IgG4 subclass might play a role in the development of WG. The MPO-ANCA in WG and MPA might recognize overlapping but different epitopes on native MPO molecule. The difference in immunological characteristics of MPO-ANCA might contribute to different disease entities.

语种英语
WOS记录号WOS:000260139300012
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65264
专题北京大学第一临床医学院
北京大学第一临床医学院_肾脏内科
作者单位Peking Univ, Peking Univ Hosp 1, Minist Hlth China,Renal Div, Inst Nephrol,Dept Med, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Liu, Li-Jun,Chen, Min,Yu, Feng,et al. IgG subclass distribution, affinity of anti-myeloperoxidase antibodies in sera from patients with Wegener&prime;s granulomatosis and microscopic polyangiitis[J]. NEPHROLOGY,2008,13(7):629-635.
APA Liu, Li-Jun,Chen, Min,Yu, Feng,Zhao, Ming-Hui,&Wang, Hai-Yan.(2008).IgG subclass distribution, affinity of anti-myeloperoxidase antibodies in sera from patients with Wegener′s granulomatosis and microscopic polyangiitis.NEPHROLOGY,13(7),629-635.
MLA Liu, Li-Jun,et al."IgG subclass distribution, affinity of anti-myeloperoxidase antibodies in sera from patients with Wegener′s granulomatosis and microscopic polyangiitis".NEPHROLOGY 13.7(2008):629-635.
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