|IgG subclass distribution, affinity of anti-myeloperoxidase antibodies in sera from patients with Wegener′s granulomatosis and microscopic polyangiitis|
|Liu, Li-Jun; Chen, Min; Yu, Feng; Zhao, Ming-Hui; Wang, Hai-Yan|
|关键词||affinity anti-myeloperoxidase antibodies epitope microscopic polyangiitis subclass Wegener&prime s granulomatosis|
|WOS标题词||Science & Technology|
|类目[WOS]||Urology & Nephrology|
|研究领域[WOS]||Urology & Nephrology|
|关键词[WOS]||CYTOPLASMIC AUTOANTIBODIES ANCA ; SYSTEMIC VASCULITIS ; MPO-ANCA ; CHINESE PATIENTS ; MYELOPEROXIDASE ; EPITOPES ; PROTEINASE-3|
Aim: Our previous study suggested that patients with M PO-AN CA (myeloperoxidase antineutrophil cytoplasmic autoantibodies)-positive Wegener′s granulomatosis (WG) were common in Chinese, indicating that patients with MPO-ANCA could manifest as either WG or microscopic polyangiitis (MPA). The aim of this study was to compare the immunological characteristics of MPO-ANCA in patients with WG and MPA.
Methods: Fifteen patients with WG and 21 patients with MPA were enrolled in the current study. Anti-MPO IgG subclasses and their titres were detected by antigen-specific enzyme-linked immunosorbent assays (ELISA); affinity was assessed by antigen-inhibition ELISAs. The sera from all patients were employed to inhibit biotin conjugated affinity-purified human anti-MPO antibodies (Probe-biotin), from plasma exchange of a patient with MPA, in a competitive inhibition ELISAs system.
Results: All four anti-MPO subclasses could be detected in sera from patients with WG and MPA. The titres of anti-MPO IgG4 subclass in patients with WG were significantly higher than those with MPA (1:1878 vs 1:218, P< 0.005).The affinity constants of MPO-ANCA were comparable between patients with WG and MPA(0.3-70/M vs 0.3-140/M respectively). Eleven out of the 15 sera and 18 out of the 21 sera could inhibit the binding of the Probe-biotin in patients with WG and MPA respectively. The average inhibition rate was 47.7% +/- 11.50% and 61.7% +/- 14.5% respectively (P < 0.05).
Conclusion: MPO-ANCA IgG4 subclass might play a role in the development of WG. The MPO-ANCA in WG and MPA might recognize overlapping but different epitopes on native MPO molecule. The difference in immunological characteristics of MPO-ANCA might contribute to different disease entities.
|作者单位||Peking Univ, Peking Univ Hosp 1, Minist Hlth China,Renal Div, Inst Nephrol,Dept Med, Beijing 100034, Peoples R China|
|Liu, Li-Jun,Chen, Min,Yu, Feng,et al. IgG subclass distribution, affinity of anti-myeloperoxidase antibodies in sera from patients with Wegener′s granulomatosis and microscopic polyangiitis[J]. NEPHROLOGY,2008,13(7):629-635.|
|APA||Liu, Li-Jun,Chen, Min,Yu, Feng,Zhao, Ming-Hui,&Wang, Hai-Yan.(2008).IgG subclass distribution, affinity of anti-myeloperoxidase antibodies in sera from patients with Wegener′s granulomatosis and microscopic polyangiitis.NEPHROLOGY,13(7),629-635.|
|MLA||Liu, Li-Jun,et al."IgG subclass distribution, affinity of anti-myeloperoxidase antibodies in sera from patients with Wegener′s granulomatosis and microscopic polyangiitis".NEPHROLOGY 13.7(2008):629-635.|