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学科主题: 临床医学
题名:
IgG subclass distribution, affinity of anti-myeloperoxidase antibodies in sera from patients with Wegener′s granulomatosis and microscopic polyangiitis
作者: Liu, Li-Jun; Chen, Min; Yu, Feng; Zhao, Ming-Hui; Wang, Hai-Yan
关键词: affinity ; anti-myeloperoxidase antibodies ; epitope ; microscopic polyangiitis ; subclass ; Wegener&prime ; s granulomatosis
刊名: NEPHROLOGY
发表日期: 2008-10-01
DOI: 10.1111/j.1440-1797.2008.00976.x
卷: 13, 期:7, 页:629-635
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: CYTOPLASMIC AUTOANTIBODIES ANCA ; SYSTEMIC VASCULITIS ; MPO-ANCA ; CHINESE PATIENTS ; MYELOPEROXIDASE ; EPITOPES ; PROTEINASE-3
英文摘要:

Aim: Our previous study suggested that patients with M PO-AN CA (myeloperoxidase antineutrophil cytoplasmic autoantibodies)-positive Wegener′s granulomatosis (WG) were common in Chinese, indicating that patients with MPO-ANCA could manifest as either WG or microscopic polyangiitis (MPA). The aim of this study was to compare the immunological characteristics of MPO-ANCA in patients with WG and MPA.

Methods: Fifteen patients with WG and 21 patients with MPA were enrolled in the current study. Anti-MPO IgG subclasses and their titres were detected by antigen-specific enzyme-linked immunosorbent assays (ELISA); affinity was assessed by antigen-inhibition ELISAs. The sera from all patients were employed to inhibit biotin conjugated affinity-purified human anti-MPO antibodies (Probe-biotin), from plasma exchange of a patient with MPA, in a competitive inhibition ELISAs system.

Results: All four anti-MPO subclasses could be detected in sera from patients with WG and MPA. The titres of anti-MPO IgG4 subclass in patients with WG were significantly higher than those with MPA (1:1878 vs 1:218, P< 0.005).The affinity constants of MPO-ANCA were comparable between patients with WG and MPA(0.3-70/M vs 0.3-140/M respectively). Eleven out of the 15 sera and 18 out of the 21 sera could inhibit the binding of the Probe-biotin in patients with WG and MPA respectively. The average inhibition rate was 47.7% +/- 11.50% and 61.7% +/- 14.5% respectively (P < 0.05).

Conclusion: MPO-ANCA IgG4 subclass might play a role in the development of WG. The MPO-ANCA in WG and MPA might recognize overlapping but different epitopes on native MPO molecule. The difference in immunological characteristics of MPO-ANCA might contribute to different disease entities.

语种: 英语
所属项目编号: 30725034 ; 30600557 ; 985-2-104-113
项目资助者: National Science fund ; National Natural Science ; Chinese 98.5 project
WOS记录号: WOS:000260139300012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65264
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: Peking Univ, Peking Univ Hosp 1, Minist Hlth China,Renal Div, Inst Nephrol,Dept Med, Beijing 100034, Peoples R China

Recommended Citation:
Liu, Li-Jun,Chen, Min,Yu, Feng,et al. IgG subclass distribution, affinity of anti-myeloperoxidase antibodies in sera from patients with Wegener&prime;s granulomatosis and microscopic polyangiitis[J]. NEPHROLOGY,2008,13(7):629-635.
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