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学科主题: 临床医学
题名:
Improvement in the quality of heterotopic allotransplanted mouse ovarian tissues with basic fibroblast growth factor and fibrin hydrogel
作者: Gao, Jiang-Man1,2; Yan, Jie1,3; Li, Rong1,2,3; Li, Min1; Yan, Li-Ying1,2; Wang, Tian-Ren1,3; Zhao, Hong-Cui1,3; Zhao, Yue1,2; Yu, Yang1,2; Qiao, Jie1,2,3
关键词: ovarian tissue ; transplantation ; basic fibroblast growth factor ; fibrin encapsulation ; angiogenesis
刊名: HUMAN REPRODUCTION
发表日期: 2013-10-01
DOI: 10.1093/humrep/det296
卷: 28, 期:10, 页:2784-2793
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: IN-VITRO ; EXTRACELLULAR-MATRIX ; SUSTAINED-RELEASE ; ACTIVIN-A ; ANGIOGENESIS ; TRANSPLANTATION ; DELIVERY ; EXPRESSION ; XENOTRANSPLANTATION ; REGENERATION
英文摘要:

Does basic fibroblast growth factor (bFGF) in combination with fibrin hydrogel improve follicle development and revascularization of heterotopically transplanted mouse ovarian tissues?

Treatment of transplanted ovarian tissues with higher concentrations (75, 100 and 150 g/ml), but not lower concentrations (25 and 50 g/ml), of bFGF significantly improved primordial follicle survival and angiogenesis, while apoptosis of follicles and stromal cells was significantly decreased.

Use of transplanted ovarian tissues in female fertility preservation is limited by the massive loss of follicles and ischemia-reperfusion injury due to the expected delay in revascularization.

Ovarian tissues from 18-day-old ICR mice were encapsulated in brin hydrogel mixed with different concentrations of bFGF, then transplanted under the skin of adult female mice for 1 week. The ovarian tissues treated without fibrin hydrogels and bFGF were designated as Control group I, and the ovarian tissues treated with fibrin hydrogels but without bFGF were designated as Control group II. The ovarian tissues treated with 25 and 50 g/ml bFGF were designated as the lower concentration group, and the ovarian tissues treated with 75, 100 and 150 g/ml bFGF were designated as the higher concentration group.

Assessment of follicular quantity and follicle classification was carried out by histologic analysis. Follicle proliferation was evidenced by immunostaining with proliferating cell nuclear antigen and apoptosis was verified by anti-active caspase-3 staining. Epithelial cells of new blood vessels were stained using CD31 antibody to evaluate neoangiogenesis, and the blood vessel density was analyzed by immunohistochemistry.

The ovarian tissues were recovered 1 week post-transplantation. Compared with the control group, the survival and proliferation of the follicles was significantly increased, the apoptosis of follicles and stromal cells was significantly decreased, and angiogenesis was significantly enhanced when the transplanted ovarian tissues were treated with a higher concentration of bFGF. Treatment with a lower concentration of bFGF did not improve follicle survival and blood revascularization.

The results obtained may not be fully extrapolated to humans because of the physiologic differences between mice and humans.

For the first time, the present study investigated the role of bFGF in transplanted ovarian tissues and demonstrated that bFGF might significantly improve the quality of transplanted ovarian tissues by increasing follicle quantity and promoting neoangiogenesis. This study sets the stage for further study and application of ovarian tissue transplantation in clinics, and may eventually benefit females for fertility preservation.

This work was partially supported by the Ministry of Science and Technology of China Grants (973 Program; 2011CB944503 to Q.J.), the Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China (30825038 to Q.J.), and the National Natural Science Funds for Young Scholar (81200470 to Y.J. and 81000275 to Y.L.Y.). None of the authors have any conflicts of interest.

语种: 英语
所属项目编号: 2011CB944503 ; 30825038 ; 81200470 ; 81000275
项目资助者: !E ieldValue">Ministry of Science and Technology of China Grants (973 Program) ; Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China ; National Natural Science Funds for Young Scholar
WOS记录号: WOS:000324836300021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65279
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100191, Peoples R China

Recommended Citation:
Gao, Jiang-Man,Yan, Jie,Li, Rong,et al. Improvement in the quality of heterotopic allotransplanted mouse ovarian tissues with basic fibroblast growth factor and fibrin hydrogel[J]. HUMAN REPRODUCTION,2013,28(10):2784-2793.
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