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学科主题: 药学
题名:
Pyrimidone derivative inhibits simian immunodeficiency virus-induced apoptosis of CEM x 174 cells
作者: Gao, Libo1; Fu, Shigan2; Li, Hui1; Wang, Xiaowei3; Liu, Junyi3; Liu, Han1; Guo, Liyuan1; Liu, Xinhua1; Li, Mengsen1; McNutt, Michael A.4; Li, Gang1
关键词: Apoptosis ; Cell cycle ; Pyrimidone ; Reverse transcriptase ; Simian immunodeficiency virus
刊名: CELL BIOLOGY INTERNATIONAL
发表日期: 2009-02-01
DOI: 10.1016/j.cellbi.2008.11.005
卷: 33, 期:2, 页:207-216
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: REVERSE-TRANSCRIPTASE INHIBITORS ; LYMPHOCYTE APOPTOSIS ; NONNUCLEOSIDE INHIBITORS ; HIV-INFECTION ; G(1) PHASE ; IN-VITRO ; TYPE-1 ; MECHANISM ; CYCLE ; NEURODEGENERATION
英文摘要:

The biochemical effects of 2-(ethoxymethylthio)-9-phenyl-cyclohepta[d] pyrimidone (EPCP), a novel non-nucleoside reverse transcriptase inhibitor, have been investigated. Treatment with EPCP (EC(50) of 0.88 nM in CEM x174 cells) significantly inhibited the activity of SIV reverse transcriptase and elevated the percentage of viable cells in an SIV-infected sample in a dose-dependent manner. The percentage of cells accumulated in G1 phase increased significantly from 34.5 to 62.4%, with a concomitant reduction in S-phase from 50.7% in the control to 22.6% in the infected group. This cell cycle profile was restored by treatment with EPCP. SIV upregulated the levels of the caspase-3, p53 and bax proteins, and downregulated the level of bcl-2 in infected cells. The apoptotic effect of SIV was also blocked by treatment with EPCP. The pharmacological effects of EPCP paralleled those of AZT, suggesting the possibility that EPCP might be a novel antiviral agent for SIV. (C) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 30621002 ; 30671856 ; 30772536
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000262980300011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65282
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Dept Biochem & Mol Biol, Hlth Sci Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Dept Biol Chem, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
3.Peking Univ, Dept Pathol, Hlth Sci Ctr, Beijing 100083, Peoples R China
4.Hainan Med Collage, Dept Pathophysiol, Haikou 570102, Peoples R China

Recommended Citation:
Gao, Libo,Fu, Shigan,Li, Hui,et al. Pyrimidone derivative inhibits simian immunodeficiency virus-induced apoptosis of CEM x 174 cells[J]. CELL BIOLOGY INTERNATIONAL,2009,33(2):207-216.
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