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学科主题基础医学
Study on prevalence and genotype of hepatitis E virus isolated from Rex Rabbits in Beijing, China
Geng, J.; Wang, L.; Wang, X.; Fu, H.; Bu, Q.; Zhu, Y.; Zhuang, H.
关键词antibody genotype hepatitis E virus rabbit
刊名JOURNAL OF VIRAL HEPATITIS
2011-09-01
DOI10.1111/j.1365-2893.2010.01341.x
18期:9页:661-667
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology ; Infectious Diseases ; Virology
研究领域[WOS]Gastroenterology & Hepatology ; Infectious Diseases ; Virology
关键词[WOS]GENOME NUCLEOTIDE-SEQUENCE ; GENETIC IDENTIFICATION ; PHYLOGENETIC ANALYSIS ; RHESUS-MONKEYS ; HEV INFECTION ; UNITED-STATES ; RT-PCR ; SWINE ; JAPAN ; DEER
英文摘要

A novel genotype of hepatitis E virus (HEV) isolated from rabbits is reported. The aim of this study was to confirm and further investigate the prevalence of the novel HEV genotype in rabbits in China. Sera and faecal samples were collected from farmed rex rabbits in Beijing, China. All serum samples were tested for anti-HEV antibody by EIA. Both the serum and the faecal samples were evaluated for detection of HEV RNA using a nested RT-PCR assay. The nucleotide sequences of rabbit HEV were then analysed, and sequence homology of rabbit HEV compared against human HEV genotypes 1-4, and avian HEV. Results: The prevalence of positive serum anti-HEV from rex rabbits was 54.62% (65/119). The detection rate of HEV RNA using ORF2 primers was 6.96% (8/115) amongst rabbit faecal samples. All eight amplicons shared 98.3-100% nucleotide homology with each other and had identities of 75.8-78.6%, 73.9-75.0%, 77.5-81.0%, 74.2-78.6% and 54.8-57.6% with the corresponding regions of genotypes 1-4 and avian HEV, respectively. Phylogenetic analysis showed that the eight sequences formed one individual branch and were on the same branch with GDC9 and GDC46, both of which were reported to be a novel genotype of HEV isolated from rabbits. The conclusion is that this study provides further information about HEV infecting rabbits, which may be a new animal host of HEV, as well as genetical evidence of a new mammalian genotype of HEV.

语种英语
WOS记录号WOS:000293191500009
项目编号2006A02Z453 ; 30570063
资助机构863 National High Technology Research and Development Program of China ; National Natural Science Foundation of China
引用统计
被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65287
专题北京大学基础医学院_病原生物学系
北京大学基础医学院
北京大学第一临床医学院_中医中西医结合科
作者单位Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Geng, J.,Wang, L.,Wang, X.,et al. Study on prevalence and genotype of hepatitis E virus isolated from Rex Rabbits in Beijing, China[J]. JOURNAL OF VIRAL HEPATITIS,2011,18(9):661-667.
APA Geng, J..,Wang, L..,Wang, X..,Fu, H..,Bu, Q..,...&Zhuang, H..(2011).Study on prevalence and genotype of hepatitis E virus isolated from Rex Rabbits in Beijing, China.JOURNAL OF VIRAL HEPATITIS,18(9),661-667.
MLA Geng, J.,et al."Study on prevalence and genotype of hepatitis E virus isolated from Rex Rabbits in Beijing, China".JOURNAL OF VIRAL HEPATITIS 18.9(2011):661-667.
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