北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 病原生物学系  > 期刊论文
学科主题: 基础医学
题名:
Study on prevalence and genotype of hepatitis E virus isolated from Rex Rabbits in Beijing, China
作者: Geng, J.; Wang, L.; Wang, X.; Fu, H.; Bu, Q.; Zhu, Y.; Zhuang, H.
关键词: antibody ; genotype ; hepatitis E virus ; rabbit
刊名: JOURNAL OF VIRAL HEPATITIS
发表日期: 2011-09-01
DOI: 10.1111/j.1365-2893.2010.01341.x
卷: 18, 期:9, 页:661-667
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology ; Infectious Diseases ; Virology
研究领域[WOS]: Gastroenterology & Hepatology ; Infectious Diseases ; Virology
关键词[WOS]: GENOME NUCLEOTIDE-SEQUENCE ; GENETIC IDENTIFICATION ; PHYLOGENETIC ANALYSIS ; RHESUS-MONKEYS ; HEV INFECTION ; UNITED-STATES ; RT-PCR ; SWINE ; JAPAN ; DEER
英文摘要:

A novel genotype of hepatitis E virus (HEV) isolated from rabbits is reported. The aim of this study was to confirm and further investigate the prevalence of the novel HEV genotype in rabbits in China. Sera and faecal samples were collected from farmed rex rabbits in Beijing, China. All serum samples were tested for anti-HEV antibody by EIA. Both the serum and the faecal samples were evaluated for detection of HEV RNA using a nested RT-PCR assay. The nucleotide sequences of rabbit HEV were then analysed, and sequence homology of rabbit HEV compared against human HEV genotypes 1-4, and avian HEV. Results: The prevalence of positive serum anti-HEV from rex rabbits was 54.62% (65/119). The detection rate of HEV RNA using ORF2 primers was 6.96% (8/115) amongst rabbit faecal samples. All eight amplicons shared 98.3-100% nucleotide homology with each other and had identities of 75.8-78.6%, 73.9-75.0%, 77.5-81.0%, 74.2-78.6% and 54.8-57.6% with the corresponding regions of genotypes 1-4 and avian HEV, respectively. Phylogenetic analysis showed that the eight sequences formed one individual branch and were on the same branch with GDC9 and GDC46, both of which were reported to be a novel genotype of HEV isolated from rabbits. The conclusion is that this study provides further information about HEV infecting rabbits, which may be a new animal host of HEV, as well as genetical evidence of a new mammalian genotype of HEV.

语种: 英语
所属项目编号: 2006A02Z453 ; 30570063
项目资助者: 863 National High Technology Research and Development Program of China ; National Natural Science Foundation of China
WOS记录号: WOS:000293191500009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65287
Appears in Collections:基础医学院_病原生物学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Study on prevalence and genotype of hepatitis E virus isolated from Rex Rabbits in Beijing, China.pdf(178KB)期刊论文出版稿限制开放 联系获取全文

作者单位: Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China

Recommended Citation:
Geng, J.,Wang, L.,Wang, X.,et al. Study on prevalence and genotype of hepatitis E virus isolated from Rex Rabbits in Beijing, China[J]. JOURNAL OF VIRAL HEPATITIS,2011,18(9):661-667.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Geng, J.]'s Articles
[Wang, L.]'s Articles
[Wang, X.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Geng, J.]‘s Articles
[Wang, L.]‘s Articles
[Wang, X.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace