|Association analysis between interleukin-1 family polymorphisms and generalized aggressive periodontitis in a Chinese population|
|Li, QY; Zhao, HS; Meng, HX; Zhang, L; Xu, L; Chen, ZB; Shi, D; Feng, XH; Zhu, XL|
|关键词||Chinese interleukin-1 periodontitis, aggressive/pathogenesis polymorphism|
|刊名||JOURNAL OF PERIODONTOLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Dentistry, Oral Surgery & Medicine|
|研究领域[WOS]||Dentistry, Oral Surgery & Medicine|
|关键词[WOS]||EARLY-ONSET PERIODONTITIS ; TUMOR-NECROSIS-FACTOR ; ADULT PERIODONTITIS ; GENE POLYMORPHISM ; CYTOKINE PRODUCTION ; GENDER-DIFFERENCES ; CIGARETTE-SMOKING ; DISEASE SEVERITY ; IL-1-BETA GENES ; PLASMA-LEVELS|
Background: It has been suggested that aggressive periodontitis (AgP) has a genetic basis, but this theory has not been confirmed. The intent of this investigation was to study whether specific interleukin (IL)-1 genotypes and/or alleles could be used to predict susceptibility to generalized AgP (GAgP) in Chinese.
Methods: The GAgP group consisted of 122 patients, and the control group included 95 healthy subjects. Single nucleotide polymorphisms at IL-1A (+4845) and IL-1B (-511, +3954) were analyzed by standard polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. The polymorphism of a variable number tandem repeat (VNTR) in intron 2 of IL-1RN was detected by PCR amplification and fragment size analysis.
Results: There was no significant association of IL-1 polymorphisms with GAgP in the unstratified subjects. However, when cases were stratified by gender, the frequencies of A2(+) genotype and allele 2 at IL-1A +4845 were significantly increased in male patients compared to male controls (genotype: odds ratio [OR] 5.58, 95% confidence interval [CI]: 1.09 to 28.68, P = 0.039; allele: OR 4.97, 95% CI: 1.01 to 24.50, P = 0.049; adjusted for age and smoking status). The frequency of IL-1B -511 A1/A2 heterozygote was significantly increased in male GAgP group compared to male controls (adjusted OR 3.16, 95% CI: 1.01 to 9.89, P = 0.048). In females, no significant differences were found between patients and controls in corresponding analyses at all polymorphic loci. A possible combined effect of IL-1B -511 polymorphism and smoking on the elevated risk to GAgP was observed. The OR of GAgP for combined A2(+) genotype and smoking was 12.45 (95% CI: 1.43 to 108.06, P = 0.022), and for combined allele 2 and smoking was 18.25 (95% CI: 2.32 to 143.86, P = 0.006).
Conclusions: The polymorphisms of IL-1A +4845 and IL-1 B -511 may play an important role in determining GAgP susceptibility in Chinese males. Furthermore, a possible combined effect of the polymorphism of IL-1 B -511 and smoking on GAgP susceptibility was suggested.
|作者单位||1.Peking Univ, Sch Stomatol, Dept Periodontol, Beijing 100081, Peoples R China|
2.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100081, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Ctr Human Dis Genomics, Beijing 100081, Peoples R China
|Li, QY,Zhao, HS,Meng, HX,et al. Association analysis between interleukin-1 family polymorphisms and generalized aggressive periodontitis in a Chinese population[J]. JOURNAL OF PERIODONTOLOGY,2004,75(12):1627-1635.|
|APA||Li, QY.,Zhao, HS.,Meng, HX.,Zhang, L.,Xu, L.,...&Zhu, XL.(2004).Association analysis between interleukin-1 family polymorphisms and generalized aggressive periodontitis in a Chinese population.JOURNAL OF PERIODONTOLOGY,75(12),1627-1635.|
|MLA||Li, QY,et al."Association analysis between interleukin-1 family polymorphisms and generalized aggressive periodontitis in a Chinese population".JOURNAL OF PERIODONTOLOGY 75.12(2004):1627-1635.|