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学科主题基础医学
Identification of a Tetrameric Assembly Domain in the C Terminus of Heat-activated TRPV1 Channels
Zhang, Feng1; Liu, Shuang1; Yang, Fan3; Zheng, Jie3; Wang, KeWei1,2
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
2011-04-29
DOI10.1074/jbc.M111.223941
286期:17页:15308-15316
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]ANKYRIN REPEAT DOMAIN ; CAPSAICIN RECEPTOR ; ION-CHANNEL ; VANILLOID RECEPTOR-1 ; PHOSPHORYLATION ; DETERMINANT ; SENSITIVITY ; SUBUNITS
英文摘要

Transient receptor potential (TRP) channels as cellular sensors are thought to function as tetramers. Yet, the molecular determinants governing channel multimerization remain largely elusive. Here we report the identification of a segment comprising 21 amino acids (residues 752-772 of mouse TRPV1) after the known TRP-like domain in the channel C terminus that functions as a tetrameric assembly domain (TAD). Purified recombinant C-terminal proteins of TRPV1-4, but not the N terminus, mediated the protein-protein interaction in an in vitro pulldown assay. Western blot analysis combined with electrophysiology and calcium imaging demonstrated that TAD exerted a robust dominant-negative effect on wild-type TRPV1. When fused with the membrane-tethered peptide Gap43, the TAD blocked the formation of stable homomultimers. Calcium imaging and current recordings showed that deletion of the TAD in a poreless TRPV1 mutant subunit suppressed its dominant-negative phenotype, confirming the involvement of the TAD in assembly of functional channels. Our findings suggest that the C-terminal TAD in TRPV1 channels functions as a domain that is conserved among TRPV1-4 and mediates a direct subunit-subunit interaction for tetrameric assembly.

语种英语
WOS记录号WOS:000289788300062
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被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65294
专题北京大学基础医学院_神经生物学系
北京大学药学院_分子与细胞药理学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China
3.Univ Calif Davis, Dept Physiol & Membrane Biol, Davis, CA 95616 USA
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GB/T 7714
Zhang, Feng,Liu, Shuang,Yang, Fan,et al. Identification of a Tetrameric Assembly Domain in the C Terminus of Heat-activated TRPV1 Channels[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2011,286(17):15308-15316.
APA Zhang, Feng,Liu, Shuang,Yang, Fan,Zheng, Jie,&Wang, KeWei.(2011).Identification of a Tetrameric Assembly Domain in the C Terminus of Heat-activated TRPV1 Channels.JOURNAL OF BIOLOGICAL CHEMISTRY,286(17),15308-15316.
MLA Zhang, Feng,et al."Identification of a Tetrameric Assembly Domain in the C Terminus of Heat-activated TRPV1 Channels".JOURNAL OF BIOLOGICAL CHEMISTRY 286.17(2011):15308-15316.
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