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学科主题: 临床医学
题名:
Performance Comparison of the Versant HCV Genotype 2.0 Assay (LiPA) and the Abbott Realtime HCV Genotype II Assay for Detecting Hepatitis C Virus Genotype 6
作者: Yang, Ruifeng; Cong, Xu; Du, Shaocai; Fei, Ran; Rao, Huiying; Wei, Lai
刊名: JOURNAL OF CLINICAL MICROBIOLOGY
发表日期: 2014-10-01
DOI: 10.1128/JCM.00882-14
卷: 52, 期:10, 页:3685-3692
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: LINE PROBE ASSAY ; SEQUENCE-ANALYSIS ; NS5B REGION ; GENERAL-POPULATION ; TMC435 MONOTHERAPY ; HIGH PREVALENCE ; HONG-KONG ; SUBTYPES ; CLASSIFICATION ; VARIANTS
英文摘要:

The Versant HCV genotype 2.0 assay (line probe assay [LiPA] 2.0), based on reverse hybridization, and the Abbott Realtime HCV genotype II assay (Realtime II), based on genotype-specific real-time PCR, have been widely used to analyze hepatitis C virus (HCV) genotypes. However, their performances for detecting HCV genotype 6 infections have not been well studied. Here, we analyzed genotype 6 in 63 samples from the China HCV Genotyping Study that were originally identified as genotype 6 using the LiPA 2.0. The genotyping results were confirmed by nonstructural 5B (NS5B) or core sequence phylogenetic analysis. A total of 57 samples were confirmed to be genotype 6 (51 genotype 6a, 5 genotype 6n, and 1 genotype 6e). Four samples identified as a mixture of genotypes 6 and 4 by the LiPA 2.0 were confirmed to be genotype 3b. The remaining two samples classified as genotype 6 by the LiPA 2.0 were confirmed to be genotype 1b, which were intergenotypic recombinants and excluded from further comparison. In 57 genotype 6 samples detected using the Realtime II version 2.00 assay, 47 genotype 6a samples were identified as genotype 6, one 6e sample was misclassified as genotype 1, and four 6a and five 6n samples yielded indeterminate results. Nine nucleotide profiles in the 5′ untranslated region affected the performances of both assays. Therefore, our analysis shows that both assays have limitations in identifying HCV genotype 6. The LiPA 2.0 cannot distinguish some 3b samples from genotype 6 samples. The Realtime II assay fails to identify some 6a and all non-6a subtypes, and it misclassifies genotype 6e as genotype 1.

语种: 英语
所属项目编号: 2012ZX10002003 ; 2012ZX10002005 ; 2012ZX09303019 ; RDC2012-06
项目资助者: China National Science and Technology Major Project for Infectious Diseases Control ; Major Project of National Science and Technology "Creation of major new drugs" ; Peking University People&prime ; s Hospital Research and Development Funds ; Bristol-Myers Squibb
WOS记录号: WOS:000342371700023
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65316
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Inst Hepatol, Beijing Key Lab Hepatitis C & Immunotherapy Liver, Beijing 100871, Peoples R China

Recommended Citation:
Yang, Ruifeng,Cong, Xu,Du, Shaocai,et al. Performance Comparison of the Versant HCV Genotype 2.0 Assay (LiPA) and the Abbott Realtime HCV Genotype II Assay for Detecting Hepatitis C Virus Genotype 6[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2014,52(10):3685-3692.
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