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学科主题: 临床医学
题名:
Suppression of renal cell carcinoma growth in vivo by forced expression of vascular endothelial growth inhibitor
作者: Zhang, Ning1; Wu, Pengjie3; Shayiremu, Duoerkun5; Wu, Liyang1; Shan, Hui1; Ye, Lin2; Zhao, Xueqin4; Cai, Jie4; Jiang, Wen Guo2; Gong, Kan3; Yang, Yong1
关键词: vascular endothelial growth inhibitor ; renal cell carcinoma ; angiogenesis ; target therapy
刊名: INTERNATIONAL JOURNAL OF ONCOLOGY
发表日期: 2013-05-01
DOI: 10.3892/ijo.2013.1877
卷: 42, 期:5, 页:1664-1673
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: TNF-LIKE LIGAND ; TUMOR-GROWTH ; ANGIOGENESIS INHIBITOR ; UNITED-STATES ; KIDNEY CANCER ; T-CELL ; TL1A ; CYTOKINE ; VEGI ; THERAPY
英文摘要:

Vascular endothelial growth inhibitor (VEGI) has been associated with tumor-related vasculature in certain malignancies. However, its implication in renal cell carcinoma (RCC), an angiogenesis-dependent tumor, remains unknown. In the present study, we investigated the role played by VEGI in RCC. The expression of VEGI was examined in human renal tissue and RCC cell lines using immunohistochemical staining and RT-PCR, respectively. The biological impact of modifying the expression of VEGI in RCC cells was evaluated using in vitro and in vivo models. We show that VEGI mRNA is expressed in a wide variety of human RCC cell lines, all of normal renal and most of RCC tissue specimens. VEGI protein expression was observed in normal renal tubular epithelial cells, but was decreased or absent in RCC specimens, particularly in tumors with high grade. Moreover, forced expression of VEGI led to an inhibition of vascular endothelial tube formation, decrease in the motility and adhesion of RCC cells in vitro. Interestingly, forced expression of VEGI had no bearing on growth, apoptosis and invasive capacity of RCC cells. However, tumor growth was reduced in xenograft models. Immunohistochemical staining showed that microvessel density decreased in VEGI forced expression xenograft tumor samples. Taken together, our findings showed that the expression of VEGI is decreased in RCC, particularly in tumors with higher grade. Together with its inhibitory effect on cellular motility, adhesion, vascular endothelial tube formation and tumor growth in vivo, this suggests that VEGI functions mainly through inhibition of angiogenesis and is a negative regulator of aggressiveness during the development and progression of RCC.

语种: 英语
所属项目编号: 81072088
项目资助者: National Natural Science Foundation of China ; Cancer Research Wales
WOS记录号: WOS:000318053400019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65318
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Capital Med Univ, Beijing Chaoyang Hosp, Dept Urol, Beijing 100020, Peoples R China
2.Cardiff Univ, Sch Med, Metastasis & Angiogenesis Res Grp, Cardiff CF14 4XN, S Glam, Wales
3.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
4.Crown Biosci Inc, Div Translat Oncol, Santa Clara, CA 95054 USA
5.Cent Hosp HaMi Reg XinJiang Prov, Dept Urol, XinJiang, Peoples R China

Recommended Citation:
Zhang, Ning,Wu, Pengjie,Shayiremu, Duoerkun,et al. Suppression of renal cell carcinoma growth in vivo by forced expression of vascular endothelial growth inhibitor[J]. INTERNATIONAL JOURNAL OF ONCOLOGY,2013,42(5):1664-1673.
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