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学科主题: 药学
题名:
Icaritin and its glycosides enhance osteoblastic, but suppress osteoclastic, differentiation and activity in vitro
作者: Huang, Jian; Yuan, Lan; Wang, Xi; Zhang, Tian-Lan; Wang, Kui
关键词: icariin ; icariside II ; icaritin ; osteoclast ; osteoblast
刊名: LIFE SCIENCES
发表日期: 2007-08-16
DOI: 10.1016/j.lfs.2007.07.015
卷: 81, 期:10, 页:832-840
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: HERBA-EPIMEDII ; CELL-GROWTH ; BONE ; OSTEOPROTEGERIN ; PHYTOESTROGENS ; ACTIVATION ; EXPRESSION ; FLAVONOIDS ; GENISTEIN ; CYTOKINE
英文摘要:

Icariin, a principal flavonoid glycoside in Herba Epimedii, is hypothesized to possess beneficial effects on bone mass. Icariin is metabolized to icariside 11 and then to icaritin in vivo. In the present study, we investigated the in vitro effects of icariin, icariside 11 and icaritin on both osteoblasts and osteoclasts. After treatment with these compounds at concentrations 10(-5)-10(-8) mol/l, ostcoblasts were examined for proliferation, alkaline phosphatase activity, osteocalcin secretion and matrix mineralization, as well as expression levels of bone-related proteins. The formation of osteoclasts was assessed by counting the number of multinucleated TRAP-positive cells. The activity of isolated rat osteoclasts was evaluated by measuring pit area, actin rings and superoxide generation. Icariside 11 and icaritin increased the mRNA expression of ALP, OC, COL-1 and OPG, but suppressed that of RANKL. In addition, these compounds reduced the number of multinucleated TRAP-positive cells and the osteoclastic resorption area. Also decreases were observed in superoxide generation and actin ring formation that are required for osteoclast survival and bone resorption activity. These findings suggest that icaritin, which was more potent than icariin and icariside 11, enhanced the differentiation and proliferation of osteoblasts, and facilitated matrix calcification; meanwhile it inhibited osteoclastic differentiation in both osteoblast-preosteoclast coculture and osteoclast progenitor cell culture, and reduced the motility and bone resorption activity of isolated osteoclasts. (c) 2007 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000249636300006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65320
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Capital Med Univ, Sch Pharmaceut Sci, Beijing 100069, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Chem Biol, Beijing 100871, Peoples R China
3.Peking Univ, Med & Hlth Anal Ctr, Beijing 100871, Peoples R China
4.Capital Med Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100069, Peoples R China

Recommended Citation:
Huang, Jian,Yuan, Lan,Wang, Xi,et al. Icaritin and its glycosides enhance osteoblastic, but suppress osteoclastic, differentiation and activity in vitro[J]. LIFE SCIENCES,2007,81(10):832-840.
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