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学科主题: 药学
题名:
Spectroscopic studies on the binding of lanthanides to the apolactoferrin
作者: Zhu, B; Du, XL; Li, RC; Wang, K; Jin, J; Wang, BC
关键词: lactoferrin ; apolactoferrin ; lanthanide ions
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2001
卷: 22, 期:1, 页:26-30
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: HUMAN-SERUM TRANSFERRIN ; HUMAN LACTOFERRIN
英文摘要:

The binding of Tb3+ and Eu3+ to apolactoferrin has been studied on the basis of the changes in differential UV, fluorescence and CD spectra, It was found that Tb3+ and Eu3+ are able to bind to two ferric-binding sites and the corresponding conditional equilibrium constants for the Tb3+ binding were determined to be lgK(1)= 8.48 +/- 0.24, lgK(2) = 6.72 +/- 0.18(0.10 mol/L NaCl, 0.10 mol/L Hepes, pH 7.4 and 25 degreesC),but Tb3+ can not displace the ferric ions from lactoferrin. By lower Tb3+/Lf ratio, the conformation became more compact with tryptophane residues exposed to more hydrophobic environment, When more Tb3+ bound to Lf, the conformation changed in other direction. Anyhow, Tb3+ binding did not affect the secondary structure.

语种: 中文
WOS记录号: WOS:000167569400006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65323
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
2.Natl Key Lab Nucl Med, Wuxi 214063, Peoples R China

Recommended Citation:
Zhu, B,Du, XL,Li, RC,et al. Spectroscopic studies on the binding of lanthanides to the apolactoferrin[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2001,22(1):26-30.
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