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Spectroscopic studies on the binding of lanthanides to the apolactoferrin
Zhu, B; Du, XL; Li, RC; Wang, K; Jin, J; Wang, BC
关键词lactoferrin apolactoferrin lanthanide ions
刊名CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
2001
22期:1页:26-30
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]HUMAN-SERUM TRANSFERRIN ; HUMAN LACTOFERRIN
英文摘要

The binding of Tb3+ and Eu3+ to apolactoferrin has been studied on the basis of the changes in differential UV, fluorescence and CD spectra, It was found that Tb3+ and Eu3+ are able to bind to two ferric-binding sites and the corresponding conditional equilibrium constants for the Tb3+ binding were determined to be lgK(1)= 8.48 +/- 0.24, lgK(2) = 6.72 +/- 0.18(0.10 mol/L NaCl, 0.10 mol/L Hepes, pH 7.4 and 25 degreesC),but Tb3+ can not displace the ferric ions from lactoferrin. By lower Tb3+/Lf ratio, the conformation became more compact with tryptophane residues exposed to more hydrophobic environment, When more Tb3+ bound to Lf, the conformation changed in other direction. Anyhow, Tb3+ binding did not affect the secondary structure.

语种中文
WOS记录号WOS:000167569400006
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65323
专题北京大学药学院_化学生物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
2.Natl Key Lab Nucl Med, Wuxi 214063, Peoples R China
推荐引用方式
GB/T 7714
Zhu, B,Du, XL,Li, RC,et al. Spectroscopic studies on the binding of lanthanides to the apolactoferrin[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2001,22(1):26-30.
APA Zhu, B,Du, XL,Li, RC,Wang, K,Jin, J,&Wang, BC.(2001).Spectroscopic studies on the binding of lanthanides to the apolactoferrin.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,22(1),26-30.
MLA Zhu, B,et al."Spectroscopic studies on the binding of lanthanides to the apolactoferrin".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 22.1(2001):26-30.
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