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学科主题: 基础医学
题名:
Sinomenine reduces invasion and migration ability in fibroblast-like synoviocytes cells co-cultured with activated human monocytic THP-1 cells by inhibiting the expression of MMP-2, MMP-9, CD147
作者: Ou, Yangqiong; Li, Weidong; Li, Xuejun; Lin, Zhibin; Li, Min
关键词: Sinomenine ; Rheumatoid arthritis ; CD147 ; Fibroblast-like synoviocytes cells ; Matrix metalloproteinase
刊名: RHEUMATOLOGY INTERNATIONAL
发表日期: 2011-11-01
DOI: 10.1007/s00296-010-1506-2
卷: 31, 期:11, 页:1479-1485
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Rheumatology
研究领域[WOS]: Rheumatology
关键词[WOS]: MATRIX-METALLOPROTEINASE INDUCER ; EMMPRIN ; COLLAGENASE ; GENE
英文摘要:

CD147 expressed by monocytes, macrophages, and synoviocytes cells can stimulate the production of matrix metalloproteinases (MMPs) associated with the development of rheumatoid arthritis (RA). We investigated the effects of Sinomenine (SIN) on invasion and migration ability and gene expression of CD147, MMP-2, MMP-9 of fibroblast-like synoviocytes cells (FLS) co-cultured with activated human monocytic THP-1 cells (A-THP-1) in vitro. SIN is a pure alkaloid extracted from the Chinese medical plant Sinomenium acutum. FLS cells were co-cultured with THP-1 cells which were induced to differentiate into macrophages with phorbol 12-myristate 13-acetate (PMA). Cells were treated with different concentrations of SIN. Invasion and migration ability of cells was tested by trans-well assays. Western blot analysis and zymographic analysis were adopted to detect the expression of CD147 and MMPs, respectively. RT-PCR was used to determine the expression of mRNA of CD147, MMP-2, and MMP-9. The invasion and migration ability of the co-cultured cells was significantly inhibited by SIN in a concentration-dependent fashion, and at the same time, the levels of CD147, MMP-2, MMP-9 were markedly down-regulated. This inhibitory effect was most notable at concentrations of 0.25 and 1.00 mM (P < 0.01). Our results point to a possible mechanism of SIN on treatment of RA is the inhibitory effect of SIN on cell invasion and migration ability, which strongly correlates with repressing the expression of CD147, MMP2, and MMP-9.

语种: 英语
WOS记录号: WOS:000297350300013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65334
Appears in Collections:基础医学院_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Lab Immunopharmacol,Dept Pharmacol, Beijing 100191, Peoples R China

Recommended Citation:
Ou, Yangqiong,Li, Weidong,Li, Xuejun,et al. Sinomenine reduces invasion and migration ability in fibroblast-like synoviocytes cells co-cultured with activated human monocytic THP-1 cells by inhibiting the expression of MMP-2, MMP-9, CD147[J]. RHEUMATOLOGY INTERNATIONAL,2011,31(11):1479-1485.
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