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学科主题: 临床医学
题名:
Role of Toll-like receptor 4 and human defensin 5 in primary endocervical epithelial cells
作者: Ma Jing-mei; Yang Hui-xia
关键词: human primary endocervical epithelial cell ; Toll-like receptor 4 ; human defensin 5
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2010-07-05
DOI: 10.3760/cma.j.issn.0366-6999.2010.13.027
卷: 123, 期:13, 页:1762-1767
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: FEMALE REPRODUCTIVE-TRACT ; GENITAL-TRACT ; NEISSERIA-GONORRHOEAE ; EXPRESSION ; IMMUNITY ; INNATE ; TOLL-LIKE-RECEPTOR-2 ; LIPOPOLYSACCHARIDE ; INDUCTION ; PROFILES
英文摘要:

Background Endocervical epithelial cells play early roles in the defense of upper female genital tract to pathogens. Toll-like receptors (TLRs) and human defensins (HD) have recently been identified as fundamental components of the innate immune responses to bacterial pathogens. We aimed to use in vitro model of human primary endocervical epithelial cells (HPECs) to investigate their roles in innate immune response of the endocervix.

Methods TLR4 expression and distribution in HPECs and endocervix were investigated by immunofluorescence (IF). Cultured HPECs were divided into lipopolysaccharide (LPS) group which were treated by LPS for 0, 24 and 48 hours, and control group without treatment. At each time point, the levels of HD5, IL-6 and TNF-alpha in supernants were determined by ELISA. TLR4 and HD5 expressions of cells were detected by Western blotting simultaneously. HD5 expression pattern was also compared between the He La cell line and HPECs.

Results Endocervix tissue surface and HPECs expressed TLR4. After incubated with LPS, HPECs expressed significantly higher levels of TLR4 than control group, especially after 24 hours (P <0.01), however decreased after 48 hours with a similar level of TLR4 expression compared with control group. LPS could upregulate the secretion of HD5, IL-6 and TNF-a in a time-dependent manner (24 hours: P <0.05; 48 hours: P <0.01, compared with control group). Intracellular HD5 expression levels decreased over time. HD5 expression patterns in HPECs were different from He La cell line.

Conclusions To respond to LPS stimulation, HPECs may function in the mucosal immune defense through TLR4 activation and HD5 secretion. HPEC is considered a significant model for immunological study. Chin Med J 2010;123(13):1762-1767

语种: 英语
WOS记录号: WOS:000280085300027
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65340
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: Peking Univ, Hosp 1, Dept Obstet & Gynaecol, Beijing 100034, Peoples R China

Recommended Citation:
Ma Jing-mei,Yang Hui-xia. Role of Toll-like receptor 4 and human defensin 5 in primary endocervical epithelial cells[J]. CHINESE MEDICAL JOURNAL,2010,123(13):1762-1767.
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