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IR@PKUHSC  > 北京大学临床肿瘤学院  > 肿瘤内科  > 期刊论文
学科主题: 临床医学
题名:
Establishment and Characterization of Multidrug-resistant Gastric Cancer Cell Lines
作者: Zhang, Xiaotian3; Yashiro, Masakazu1,2; Qiu, Hong4; Nishii, Takafumi; Matsuzaki, Taro; Hirakawa, Kosei
关键词: Chemotherapy ; cross-resistance ; gastric cancer ; anticancer drugs ; apoptosis ; cell-cycle
刊名: ANTICANCER RESEARCH
发表日期: 2010-03-01
卷: 30, 期:3, 页:915-921
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: DIHYDROPYRIMIDINE DEHYDROGENASE ; FOLINIC ACID ; FLUOROURACIL ; CHEMOTHERAPY ; CISPLATIN ; ADENOCARCINOMA ; OXALIPLATIN ; COMBINATION ; GEMCITABINE ; DRUGS
英文摘要:

Aim: The aim of this study was to establish drug-resistant cell lines and to elucidate mechanisms leading to multi-drug resistance in gastric cancer. Materials and Methods: Five cancer cell lines resistant to 5-fluorouracil, paclitaxel, oxaliplatin, irinotecan, or gemcitabine, were respectively established from a parent gastric cancer cell line, OCUM-2M, by stepwise exposure to each chemotherapeutical agent. Results: Cell death by apoptosis induced by anti-cancer drugs was low in 5 chemo-resistant cell lines. Percentage of cells in S and G(0)/G(1) phase was low in cell lines resistant to oxaliplatin or irinotecan. Cell lines resistant to paclitaxel, oxaliplatin, and gemcitabine showed multi-drug resistance. Alterations in MRP, DAPK1, or DAPK2 expression were found in multi-drug resistant cell lines. Conclusion: The cell-cycle distribution and alterations of MRP, DAPK1, and DAPK2 genes may be integral part of mechanisms responsible for chemo-resistance. These cell lines might be useful to study molecular mechanisms leading to multi-drug resistance.

语种: 英语
所属项目编号: 18591475 ; 20591073 ; 18390369
项目资助者: Japan-China Sasagawa Medical Scholarship ; KAKENHI ; JSGE Grant-in Aid for Scientific Research ; Grant-in Aid for Kobayashi Foundation for Innovative Cancer Chemotherapy ; Grant-in Aid for the Sagawa Foundation for Cancer Research
WOS记录号: WOS:000276561300029
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65345
Appears in Collections:北京大学临床肿瘤学院_肿瘤内科_期刊论文

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作者单位: 1.Osaka City Univ, Grad Sch Med, Dept Surg Oncol, Abeno Ku, Osaka 5458585, Japan
2.Osaka City Univ, Grad Sch Med, Oncol Inst Geriatr & Med Sci, Osaka 5458585, Japan
3.Peking Univ, Dept Med Oncol, Beijing Canc Hosp, Sch Oncol, Beijing 100871, Peoples R China
4.Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Ctr Oncol, Wuhan 430074, Peoples R China

Recommended Citation:
Zhang, Xiaotian,Yashiro, Masakazu,Qiu, Hong,et al. Establishment and Characterization of Multidrug-resistant Gastric Cancer Cell Lines[J]. ANTICANCER RESEARCH,2010,30(3):915-921.
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