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Anti-angiogenic effect of KH902 on retinal neovascularization
Wang, Fei1; Bai, Yujing1; Yu, Wenzhen1; Han, Na2; Huang, Lvzhen1; Zhao, Min1; Zhou, Aiyi1; Zhao, Mingwei1; Li, Xiaoxin1
关键词KH902 Angiogenesis Retinopathy
刊名GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
2013-09-01
DOI10.1007/s00417-013-2392-6
251期:9页:2131-2139
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]ENDOTHELIAL GROWTH-FACTOR ; PROLIFERATIVE DIABETIC-RETINOPATHY ; VASCULAR-PERMEABILITY FACTOR ; INTRAVITREAL INJECTION ; MULTICENTER TRIAL ; FUSION PROTEIN ; VEGF-BINDING ; PREMATURITY ; EXPRESSION ; RECEPTOR
英文摘要

KH902 is a fusion protein derived from the extracellular domains of vascular endothelial growth factor (VEGF) receptors 1 and 2 and the Fc portion of immunoglobulin G1 (IgG1). Retinopathy of prematurity (ROP) is an eye disease that affects premature babies who have received intensive neonatal care, and the disorganization of retinal blood vessels may result in scarring and retinal detachment. This study was designed to examine the inhibitory effects of KH902 on mice with oxygen-induced retinopathy (OIR), one of the animal models of ROP.

Human umbilical vein endothelial cells (HUVECs) were used for an in vitro study, and the C57BL/6 J OIR mouse model was used for an in vivo study. HUVECs were incubated with KH902 or a VEGF- and KH902-containing medium. Cell proliferation, migration, apoptosis, and tube formation were measured with BrdU incorporation, Transwell, flow cytometry, and Matrigel assays. C57BL/6 J mice were exposed to 75 % oxygen from postnatal day 7 (P7) to P12, after which the mice were brought to room air and intravitreously injected with KH902. At P18, the mice were perfused with fluorescein isothiocyanate (FITC)-dextran and Evans Blue, and flat-mounted retinas were used to measure the non-perfused and leakage areas. The data were analyzed with GraphPad Prism 5.0 software.

In vitro, KH902 dose-dependently inhibited HUVEC proliferation in general culture medium and in VEGF(165)-containing medium at different time points. Moreover, KH902 inhibited HUVEC migration and tube formation and induced HUVEC apoptosis. In vivo, an intravitreous injection of KH902 reduced the retinal non-perfused area from 34 % in the control group to 19 % in the treatment group and significantly reduced the retinal leakage area from 18 % to 9 %.

KH902 had marked inhibitory effects on angiogenesis both in vitro and in vivo. These data suggest that KH902 could serve as an innovative pharmaceutical agent to prevent retinal neovascularization (NV) and as a strategy for the treatment of ROP.

语种英语
WOS记录号WOS:000324114500007
项目编号RDB2012-24 ; 2011CB510200
资助机构Peking University People&prime ; s Hospital Research and Development Fund for BYJ ; National Basic Research Program of China (973 Program)
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65351
专题北京大学第二临床医学院_眼科
作者单位1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Key Lab Vis Loss & Restorat,Minist Educ, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Orthoped & Trauma, Beijing 100044, Peoples R China
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GB/T 7714
Wang, Fei,Bai, Yujing,Yu, Wenzhen,et al. Anti-angiogenic effect of KH902 on retinal neovascularization[J]. GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY,2013,251(9):2131-2139.
APA Wang, Fei.,Bai, Yujing.,Yu, Wenzhen.,Han, Na.,Huang, Lvzhen.,...&Li, Xiaoxin.(2013).Anti-angiogenic effect of KH902 on retinal neovascularization.GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY,251(9),2131-2139.
MLA Wang, Fei,et al."Anti-angiogenic effect of KH902 on retinal neovascularization".GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY 251.9(2013):2131-2139.
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