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学科主题: 口腔医学
题名:
Characterization of Dental Pulp Stem Cells Isolated from Canine Premolars
作者: Dissanayaka, Waruna Lakmal1; Zhu, Xiaofei2,3; Zhang, Chengfei1; Jin, Lijian1
关键词: Canine animal model ; dental pulp stem cells ; regenerative endodontics ; tissue engineering
刊名: JOURNAL OF ENDODONTICS
发表日期: 2011-08-01
DOI: 10.1016/j.joen.2011.04.004
卷: 37, 期:8, 页:1074-1080
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: SIDE POPULATION CELLS ; IN-VITRO ; SIALOPHOSPHOPROTEIN GENE ; SELF-RENEWAL ; BONE-MARROW ; DIFFERENTIATION ; TRANSPLANTATION ; REGENERATION ; EXPRESSION ; ANTIBODIES
英文摘要:

Introduction: Dogs are commonly used animal models for regenerative endodontics research. Although several studies have used stem cells isolated from dog teeth to investigate the dentin/pulp regeneration in vivo, less attention has been paid for the characterization of these cells. Therefore, this study aimed to characterize the dental pulp stem cells isolated from dog teeth (cDPSCs) in order to further define the dog as an animal model for regenerative endodontics. Methods: Stem cells were isolated from freshly extracted premolars of 10-month-old Beagles. The isolated cells were investigated for their stem cell properties by analysis of their clonogenic and growth characteristics; expression of mesenchymal stem cell markers; and evaluation of their osteo/odontogenic, adipogenic, and neurogenic potential. Results: A colony formation assay showed the existence of a clonogenic cell population in cDPSCs isolated. The growth curves revealed a higher proliferation rate of cDPSCs compared with hBMMSCs. cDPSCs expressed mesenchymal stem cell markers STRO-1, CD146, and Nanog. However, they were negative for CD73, CD105, and CD45. cDPSCs were able to differentiate into odontoblast-like cells as shown by increased alkaline phosphatase activity, dentin sialoprotein expression, and formation of mineralized nodules. cDPSCs also showed the neurogenic and adipogenic differentiation potential at a lower level compared with those of hDPSCs and hBMMSCs. Conclusions: The results of this study confirmed the stem cell properties of cDPSCs at a comparable level to those of hDPSCs and hBMMSCs. Overall, the data presented in this study provide evidence in supportive of using cDPSCs and dogs as an animal model in dental tissue engineering via stem cell based approaches. (J Endod 2011;37:1074-1080)

语种: 英语
所属项目编号: 200910159002 ; 2009DFA32950
项目资助者: The University of Hong Kong, Hong Kong SAR, China ; International Cooperation in Science and Technology Cooperation Project, Beijing, China
WOS记录号: WOS:000294091500007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65369
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Univ Hong Kong, Fac Dent, Hong Kong, Hong Kong, Peoples R China
2.Peking Univ, Dept Special Dent Serv, Sch Stomatol, Beijing 100871, Peoples R China
3.Peking Univ, Dept Special Dent Serv, Hosp Stomatol, Beijing 100871, Peoples R China

Recommended Citation:
Dissanayaka, Waruna Lakmal,Zhu, Xiaofei,Zhang, Chengfei,et al. Characterization of Dental Pulp Stem Cells Isolated from Canine Premolars[J]. JOURNAL OF ENDODONTICS,2011,37(8):1074-1080.
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