|Beijing ambient particle exposure accelerates atherosclerosis in ApoE knockout mice|
|Chen, Tian1; Jia, Guang1; Wei, Yongjie2,3,4; Li, Jiucun1|
|关键词||Ambient particle Atherosclerosis ApoE knockout mice Systemic inflammation Oxidative stress Lipid metabolism|
|WOS标题词||Science & Technology|
|关键词[WOS]||LOW-DENSITY-LIPOPROTEIN ; MATTER AIR-POLLUTION ; CORONARY-ARTERY-DISEASE ; PARTICULATE MATTER ; SUBCLINICAL ATHEROSCLEROSIS ; CARDIOVASCULAR-DISEASE ; OXIDIZED LDL ; RESIDENTIAL EXPOSURE ; PROGNOSTIC MARKER ; OXIDATIVE STRESS|
Background: Air pollution is associated with significant adverse health effects including increased cardiovascular morbidity and mortality. However research on the cardiovascular effect of "real-world" exposure to ambient particulate matter (PM) in susceptible animal model is very limited. In this study, we aimed to investigate the association between Beijing ambient particle exposure and the atherosclerosis development in the apolipoprotein E knockout mice (ApoE-/-mice).
Methods: Two parallel exposure chambers were used for whole body exposure among ApoE knockout mice. One of the chambers was supplied with untreated ambient air (PM group) and the other chamber was treated with ambient air filtered by high-efficiency particulate air (HEPA) filter (FA group). Twenty mice were divided into two groups and exposed to ambient PM (n = 10 for PM group) or filtered air(n = 10 for FA group) for two months from January 18th to March 18th, 2010. During the exposure, the mass concentrations of PM2.5 and PM10 in the two chambers were continuously monitored. Additionally, a receptor source apportionment model of chemical mass balance using 19 organic tracers was applied to determine the contributions of sources on the PM2.5 in terms of natural gas, diesel vehicle, gasoline vehicle, coal burning, vegetable debris, biomass burning and cooking. At the end of the two-month exposure, biomarkers of oxidative stress, inflammation and lipid metabolism in bronchoalveolar lavage fluid (BAL) and blood samples were determined and the plaque area on the aortic endothelium was quantified.
Results: In the experiment, the concentrations of PM10 and PM2.5 in PM chamber were 99.45 mu g/m(3) and 61.0 mu g/m(3) respectively, while PM2.5 in FA chamber was 17.6 mu g/m(3). Source apportionment analysis by organic tracers showed that gasoline vehicle (39.9%) and coal burning (24.3%) emission were the two major sources contributing to the mass concentration of PM2.5 in Beijing. Among the ApoE knockout mice, the PM group were significantly higher than the FA group in terms of serum total cholesterol, low-density lipoprotein, tumor necrosis factor-alpha (TNF-alpha) and C-reactive protein as well as TNF-alpha and interleukin-6 in BAL. Also the total antioxidant capacity and oxidized low-density lipoprotein were significantly different between the two groups. In addition, pathological analysis of aortic arch reveals that the plaques area in the PM group increased significantly compared to the FA group.
Conclusions: Our results demonstrated that ambient PM exposure could induce considerable oxidative stress and systemic inflammation in ApoE knockout mice and contribute to the progression of atherosclerosis. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
|资助机构||National Natural Science Foundation of China ; State Key Joint Laboratory of Environmental Simulation and Pollution Control (Peking University)|
|作者单位||1.Peking Univ, Dept Occupat & Environm Hlth Sci, Sch Publ Hlth, Beijing 100191, Peoples R China|
2.Chinese Res Inst Environm Sci, Sate Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China
3.Chinese Res Inst Environm Sci, Environm Stand Inst, Beijing 100012, Peoples R China
4.Peking Univ, State Key Joint Lab Environm Simulat & Pollut Con, Coll Environm Sci & Engn, Beijing 100871, Peoples R China
|Chen, Tian,Jia, Guang,Wei, Yongjie,et al. Beijing ambient particle exposure accelerates atherosclerosis in ApoE knockout mice[J]. TOXICOLOGY LETTERS,2013,223(2):146-153.|
|APA||Chen, Tian,Jia, Guang,Wei, Yongjie,&Li, Jiucun.(2013).Beijing ambient particle exposure accelerates atherosclerosis in ApoE knockout mice.TOXICOLOGY LETTERS,223(2),146-153.|
|MLA||Chen, Tian,et al."Beijing ambient particle exposure accelerates atherosclerosis in ApoE knockout mice".TOXICOLOGY LETTERS 223.2(2013):146-153.|