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Headspace solid-phase microextraction combined with GC x GC-TOFMS for the analysis of volatile compounds of Coptis species rhizomes
Gao, Xin1,2; Yang, Xiuwei2; Mitrevski, Blagoj S.1; Marriott, Philip J.1
关键词GC x GC-TOFMS Headspace solid-phase microextraction Rhizome of Coptis species Volatile compounds
刊名JOURNAL OF SEPARATION SCIENCE
2011-05-01
DOI10.1002/jssc.201100022
34期:10页:1157-1166
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
资助者China Scholarship Council ; Australian Research Council ; China Scholarship Council ; Australian Research Council
研究领域[WOS]Chemistry
关键词[WOS]2-DIMENSIONAL GAS-CHROMATOGRAPHY ; ACCELERATED SOLVENT-EXTRACTION ; FLIGHT MASS-SPECTROMETRY ; CAPILLARY-ELECTROPHORESIS ; CHINENSIS FRANCH ; BERBERINE ; ALKALOIDS ; CELLS
英文摘要

In this study, the investigation of the volatile compounds of dried rhizomes of Coptis chinensis Franch, C. deltoidea C. Y. Cheng et Hsiao, and C. teeta Wall was carried out to complete the chemical composition of these valuable natural products. Volatile profiles were established and compared after headspace solid-phase microextraction (HS-SPME) using a polydimethylsiloxane/divinylbenzene (PDMS/DVB, 65 mm) fibre coupled to comprehensive 2D gas chromatography time-of-flight mass spectrometry (GC x GC-TOFMS). Analyses were performed and compared on two column-phase combinations (non-polar/polar and polar/non-polar). The majority of the identified compounds eluted as well-separated (pure) components as a result of high-resolution capability of the GC x GC method, which significantly reduces co-elution. Therefore, this increases the likelihood that pure mass spectra can be obtained. More than 290 volatile and semivolatile organic compounds were tentatively characterized by means of GC x GC in tandem with TOFMS detection. Improved result interpretations were obtained in terms of compound classification based on the organized structure of the peaks of structurally related compounds in the GC x GC contour plot. These compounds are distributed over the chemical groups of hydrocarbons, acids, alkenes, alkynes, aldehydes, ketones, alcohols, esters, furans, and terpenoids. Among all the chemical groups, terpenoids present the higher number of identified compounds (44), alkenes (41), and aldehydes and ketones (28). This study completed the volatile phytochemical analysis of the headspace composition of various Coptis species rhizomes, and should serve as a means to identify the difference between the rhizomes and may also be useful to confirm individual species based on their volatile chemical profile.

语种英语
所属项目编号LE0775725
资助者China Scholarship Council ; Australian Research Council ; China Scholarship Council ; Australian Research Council
WOS记录号WOS:000290635400009
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65375
Collection北京大学药学院
作者单位1.Monash Univ, Ctr Green Chem, Sch Chem, Clayton, Vic 3800, Australia
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Hlth Sci Ctr, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Gao, Xin,Yang, Xiuwei,Mitrevski, Blagoj S.,et al. Headspace solid-phase microextraction combined with GC x GC-TOFMS for the analysis of volatile compounds of Coptis species rhizomes[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(10):1157-1166.
APA Gao, Xin,Yang, Xiuwei,Mitrevski, Blagoj S.,&Marriott, Philip J..(2011).Headspace solid-phase microextraction combined with GC x GC-TOFMS for the analysis of volatile compounds of Coptis species rhizomes.JOURNAL OF SEPARATION SCIENCE,34(10),1157-1166.
MLA Gao, Xin,et al."Headspace solid-phase microextraction combined with GC x GC-TOFMS for the analysis of volatile compounds of Coptis species rhizomes".JOURNAL OF SEPARATION SCIENCE 34.10(2011):1157-1166.
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