IR@PKUHSC  > 北京大学第二临床医学院  > 妇科肿瘤中心
学科主题临床医学
Low Programmed Cell Death 5 Expression is a Prognostic Factor in Ovarian Cancer
Gao, Li1; Ye, Xue1; Ma, Rui-Qiong1; Cheng, Hong-Yan1; Han, Hong-Jing2; Cui, Heng1; Wei, Li-Hui1; Chang, Xiao-Hong1
关键词Ovarian Cancer Poor Prognosis Programmed Cell Death 5
刊名CHINESE MEDICAL JOURNAL
2015-04-20
DOI10.4103/0366-6999.155100
128期:8页:1084-1090
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
资助者&prime ; &prime ; 863&prime ; &prime ; National High Technology and Development Program ; &prime ; &prime ; 863&prime ; &prime ; National High Technology and Development Program
研究领域[WOS]General & Internal Medicine
关键词[WOS]BREAST-CANCER ; TUMOR-CELLS ; PDCD5 ; GENE ; APOPTOSIS ; INTERACTS ; PROFILES ; LEUKEMIA
英文摘要

Background: Ovarian cancer is a leading gynecological malignancy. We investigated the prognostic value of programmed cell death 5 (PDCD5) in patients with ovarian cancer.

Methods: Expression levels of PDCD5 mRNA and protein were examined in six ovarian cancer cell lines (SKOV3, CAOV3, ES2, OV1, 3AO, and HOC1A) and one normal ovarian epithelial cell line (T29) using reverse transcription polymerase chain reaction, Western blotting, and flow cytometry. After inducing PDCD5 induction in SKOV3 cells or treating this cell line with taxol or doxorubicin (either alone or combined), apoptosis was measured by Annexin V-FITC/propidium iodide staining. Correlations between PDCD5 protein expression and pathological features, histological grade, FIGO stage, effective cytoreductive surgery, and serum cancer antigen-125 values were evaluated in patients with ovarian cancer.

Results: PDCD5 mRNA and protein expression were downregulated in ovarian cancer cells. Recombinant human PDCD5 increased doxorubicin-induced apoptosis in SKOV3 cells (15.96 +/- 2.07%, vs. 3.17 +/- 1.45% in controls). In patients with ovarian cancer, PDCD5 expression was inversely correlated with FIGO stage, pathological grade, and patient survival (P < 0.05, R = 0.7139 for survival).

Conclusions: PDCD5 expression is negatively correlated with disease progression and stage in ovarian cancer. Therefore, measuring PDCD5 expression may be a good method of determining the prognosis of ovarian cancer patients.

语种英语
所属项目编号2012AA02A507
资助者&prime ; &prime ; 863&prime ; &prime ; National High Technology and Development Program ; &prime ; &prime ; 863&prime ; &prime ; National High Technology and Development Program
WOS记录号WOS:000353563300016
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65380
Collection北京大学第二临床医学院_妇科肿瘤中心
作者单位1.Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Ctr Reprod Med, Beijing 100044, Peoples R China
Recommended Citation
GB/T 7714
Gao, Li,Ye, Xue,Ma, Rui-Qiong,et al. Low Programmed Cell Death 5 Expression is a Prognostic Factor in Ovarian Cancer[J]. CHINESE MEDICAL JOURNAL,2015,128(8):1084-1090.
APA Gao, Li.,Ye, Xue.,Ma, Rui-Qiong.,Cheng, Hong-Yan.,Han, Hong-Jing.,...&Chang, Xiao-Hong.(2015).Low Programmed Cell Death 5 Expression is a Prognostic Factor in Ovarian Cancer.CHINESE MEDICAL JOURNAL,128(8),1084-1090.
MLA Gao, Li,et al."Low Programmed Cell Death 5 Expression is a Prognostic Factor in Ovarian Cancer".CHINESE MEDICAL JOURNAL 128.8(2015):1084-1090.
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