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学科主题基础医学
Comparison of Abbott and Da-an real-time PCR for quantitating serum HBV DNA
Qiu, Ning1,2; Li, Rui1,2; Yu, Jian-Guo3; Yang, Wen3; Zhang, Wei3; An, Yong3; Li, Tong1,2; Liu, Xue-En1,2; Zhuang, Hui1,2
关键词Hepatitis B virus Hepatitis B virus DNA quantitation Real-time polymerase chain reaction Chronic hepatitis B Antiviral therapy
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2014-09-07
DOI10.3748/wjg.v20.i33.11762
20期:33页:11762-11769
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]CHRONIC HEPATITIS-B ; VIRUS DNA ; 2008 UPDATE ; QUANTIFICATION ; MANAGEMENT ; ASSAY ; INFECTION
英文摘要

AIM: To compare the performance of the Da-an real-time hepatitis B virus (HBV) DNA assay and Abbott Real-Time HBV assay.

METHODS: HBV DNA standards as well as a total of 180 clinical serum samples from patients with chronic hepatitis B were measured using the Abbott and Daan real-time polymerase chain reaction (PCR) assays. Correlation and Bland-Altman plot analysis was used to compare the performance of the Abbott and Daan assays. The HBV DNA levels were logarithmically transformed for analysis. All statistical analyses were performed using SPSS for Windows version 18.0. The correlation between the two assays was analyzed by Pearson′s correlation and linear regression. The Bland-Altman plots were used for the analysis of agreement between the two assays. A P value of < 0.05 was considered statistically significant.

RESULTS: The HBV DNA values measured by the Abbott or Da-an assay were significantly correlated with the expected values of HBV DNA standards (r = 0.999, for Abbott; r = 0.987, for Da-an, P < 0.001). A Bland-Altman plot showed good agreement between these two assays in detecting HBV DNA standards. Among the 180 clinical serum samples, 126 were quantifiable by both assays. Fifty-two samples were detectable by the Abbott assay but below the detection limit of the Da-an assay. Moreover, HBV DNA levels measured by the Abbott assay were significantly higher than those of the Da-an assay (6.23 +/- 1.76 log IU/mL vs 5.46 +/- 1.55 log IU/mL, P < 0.001). A positive correlation was observed between HBV DNA concentrations determined by the two assays in 126 paired samples (r = 0.648, P < 0.001). One hundred and fifteen of 126 (91.3%) specimens tested with both assays were within mean difference +/- 1.96 SD of HBV DNA levels.

CONCLUSION: The Da-an assay presented lower sensitivity and a narrower linear range as compared to the Abbott assay, suggesting the need to be improved. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.

语种英语
WOS记录号WOS:000341718600026
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65381
专题北京大学基础医学院_病原生物学系
北京大学基础医学院
北京大学第一临床医学院_手术室
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Microbiol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Ctr Infect Dis, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Peoples Liberat Army, Hosp 88, Liver Dis Ctr, Dept Internal Med, Tai An 27100, Shandong, Peoples R China
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GB/T 7714
Qiu, Ning,Li, Rui,Yu, Jian-Guo,et al. Comparison of Abbott and Da-an real-time PCR for quantitating serum HBV DNA[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2014,20(33):11762-11769.
APA Qiu, Ning.,Li, Rui.,Yu, Jian-Guo.,Yang, Wen.,Zhang, Wei.,...&Zhuang, Hui.(2014).Comparison of Abbott and Da-an real-time PCR for quantitating serum HBV DNA.WORLD JOURNAL OF GASTROENTEROLOGY,20(33),11762-11769.
MLA Qiu, Ning,et al."Comparison of Abbott and Da-an real-time PCR for quantitating serum HBV DNA".WORLD JOURNAL OF GASTROENTEROLOGY 20.33(2014):11762-11769.
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