北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 病原生物学系  > 期刊论文
学科主题: 基础医学
题名:
Comparison of Abbott and Da-an real-time PCR for quantitating serum HBV DNA
作者: Qiu, Ning1,2; Li, Rui1,2; Yu, Jian-Guo3; Yang, Wen3; Zhang, Wei3; An, Yong3; Li, Tong1,2; Liu, Xue-En1,2; Zhuang, Hui1,2
关键词: Hepatitis B virus ; Hepatitis B virus DNA quantitation ; Real-time polymerase chain reaction ; Chronic hepatitis B ; Antiviral therapy
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2014-09-07
DOI: 10.3748/wjg.v20.i33.11762
卷: 20, 期:33, 页:11762-11769
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: CHRONIC HEPATITIS-B ; VIRUS DNA ; 2008 UPDATE ; QUANTIFICATION ; MANAGEMENT ; ASSAY ; INFECTION
英文摘要:

AIM: To compare the performance of the Da-an real-time hepatitis B virus (HBV) DNA assay and Abbott Real-Time HBV assay.

METHODS: HBV DNA standards as well as a total of 180 clinical serum samples from patients with chronic hepatitis B were measured using the Abbott and Daan real-time polymerase chain reaction (PCR) assays. Correlation and Bland-Altman plot analysis was used to compare the performance of the Abbott and Daan assays. The HBV DNA levels were logarithmically transformed for analysis. All statistical analyses were performed using SPSS for Windows version 18.0. The correlation between the two assays was analyzed by Pearson′s correlation and linear regression. The Bland-Altman plots were used for the analysis of agreement between the two assays. A P value of < 0.05 was considered statistically significant.

RESULTS: The HBV DNA values measured by the Abbott or Da-an assay were significantly correlated with the expected values of HBV DNA standards (r = 0.999, for Abbott; r = 0.987, for Da-an, P < 0.001). A Bland-Altman plot showed good agreement between these two assays in detecting HBV DNA standards. Among the 180 clinical serum samples, 126 were quantifiable by both assays. Fifty-two samples were detectable by the Abbott assay but below the detection limit of the Da-an assay. Moreover, HBV DNA levels measured by the Abbott assay were significantly higher than those of the Da-an assay (6.23 +/- 1.76 log IU/mL vs 5.46 +/- 1.55 log IU/mL, P < 0.001). A positive correlation was observed between HBV DNA concentrations determined by the two assays in 126 paired samples (r = 0.648, P < 0.001). One hundred and fifteen of 126 (91.3%) specimens tested with both assays were within mean difference +/- 1.96 SD of HBV DNA levels.

CONCLUSION: The Da-an assay presented lower sensitivity and a narrower linear range as compared to the Abbott assay, suggesting the need to be improved. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.

语种: 英语
所属项目编号: 2013ZX10002004 ; 2012ZX10002003
项目资助者: Major Science and Technology Special Project of China Twelfth Five-year Plan
WOS记录号: WOS:000341718600026
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65381
Appears in Collections:基础医学院_病原生物学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Comparison of Abbott and Da-an real-time PCR for quantitating serum HBV DNA.pdf(920KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Microbiol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Ctr Infect Dis, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Peoples Liberat Army, Hosp 88, Liver Dis Ctr, Dept Internal Med, Tai An 27100, Shandong, Peoples R China

Recommended Citation:
Qiu, Ning,Li, Rui,Yu, Jian-Guo,et al. Comparison of Abbott and Da-an real-time PCR for quantitating serum HBV DNA[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2014,20(33):11762-11769.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Qiu, Ning]'s Articles
[Li, Rui]'s Articles
[Yu, Jian-Guo]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Qiu, Ning]‘s Articles
[Li, Rui]‘s Articles
[Yu, Jian-Guo]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace